Using a new pH sensor, we found that CO2 reaches the NVU within 2 sec ! And that GCaMP is a good reporter of acidosis (β οΈ). But the dilation to CO2 lags acidosis by 3-4 sec
π¬Our H-Rubies have struck againπͺ! H-Ruby conjugated dextran in combination with AF488 enabled measuring vascular pH variations in in vivo 2-photon microscopy. Check it out in @NatureComms.π@sonia_pfister π@AgenceRecherche
https://t.co/mZ4qL5Ayat
We are finally online π2P-FENDO for selective
optogenetics photostimulation and high-speed calcium imaging in freely moving
mice! Congrats to @EmilianiLab and Ori Katz lab! https://t.co/zWXtpBxS0d
In addition to a detailed protocol, we provided exemples of functional imaging with two-photon, fUS and BOLD fMRI.
High imaging stability and durability of the pep. is achieved thanks to a U-shape head bar.