Matt Witkowsky

Capstone 2020. Chief Executive Research Associate at Xpress Bio

[Matt Witkowsky @MattWitkowsky]

@ScottS_Biochar @NU_ChemE @BrendanWhalen14 @DerkNasty_ @swolerito @MarcZoleta Hi Scott,

As the protein A chromatography unit accounts for the highest cost of waste in the batch unit, a lot of our work went into reducing the cost there. By reducing column size, and thus resin/buffer requirements, we were able to have the most considerable savings there.

[Matt Witkowsky @MattWitkowsky]

Xpress Bio (Group #15): Modular Continuous Process for Purification of Monoclonal Antibodies #CHME4703Poster @NU_ChemE

[Matt Witkowsky @MattWitkowsky]

@chris_gagnon88 @NU_ChemE @BrendanWhalen14 @DerkNasty_ @swolerito @MarcZoleta Hi Chris,

This process could be scaled up for a higher throughput process. Major factors we'd have to account for in scale-up in purification would be understanding column breakthrough at a higher scale and flowrate as well as filter membrane performance in diafiltration.

[Matt Witkowsky @MattWitkowsky]

@renegold8 @NU_ChemE @BrendanWhalen14 @DerkNasty_ @swolerito @MarcZoleta A majority of our buffer use, thus comes from these chromatography sections. We understand there's a lot of components added/taken out of the process, so we've defined our metrics in terms of waste per kg mAb to have a hopefully more understandable comparison to the batch case.

[Matt Witkowsky @MattWitkowsky]

@renegold8 @NU_ChemE @BrendanWhalen14 @DerkNasty_ @swolerito @MarcZoleta For all chromatography sections, waste is generated through different buffers used for separate column phases. The process works through switching buffer lines on/off (load, wash, equil, regeneration) dependent on the column phase to purify the product.

[Matt Witkowsky @MattWitkowsky]

@renegold8 @NU_ChemE @BrendanWhalen14 @DerkNasty_ @swolerito @MarcZoleta Hi Rene,

We do have total stream summaries, however, we thought it would be a bit to overwhelming for the poster, as we have about 100 defined streams.

[Matt Witkowsky @MattWitkowsky]

@ThomasJWebster1 @NU_ChemE @BrendanWhalen14 @DerkNasty_ @swolerito @MarcZoleta Hi Professor,

Our process was modeled based on batch-case parameters and materials for the Humira manufacturing process described in their patent. We've chosen Humira as a case study as it is continually the highest selling mAb with the most available data.

[Matt Witkowsky @MattWitkowsky]

Unit 5: Diafiltration

[Matt Witkowsky @MattWitkowsky]

Unit 4: Anion Exchange Chromatography