It was great fun to work on this preprint with @robmlees@ibianco3 @raacampbell @natorlova Darcy Peterka @labrigger@YatsenkoDimitri Che-Hang Yu & @paqio.
Hope this helps many people to characterise their 2p microscopes https://t.co/oGBfUNNkud
Microscope standards started in 1858, and they’re still constraining optics. But we can break free. Nature Methods also published this little accompanying backstory / commentary on the Cousa: free readcube link https://t.co/aCXwsJOE2l and regular link https://t.co/kJxozGbokt.
Out yesterday from the Yu and Smith labs! The Cousa objective is an ultra-long working distance air objective optimized for 2P and 3P imaging that opens the door to new imaging experiments, including in non-human primates. https://t.co/urGxbYeryU
The Cousa objective: Having 20 mm of working distance in air can enable new experiments for two-photon imaging, particularly challenging experiments in living animals. The Cousa manuscript is out now. Open access. https://t.co/QFXYvcfFJn (1/n)
Updated preprint for the ultra long working distance Cousa objective. 20 mm air immersion, 2 mm x 2 mm field-of-view, 0.5 NA. https://t.co/bO9Cwq6cRZ
Blog post on it: https://t.co/QiCSKalC2Z
Johannes Friedrich – “Online methods for real-time analysis of calcium imaging data”
We will present online adaptations of the CNMF as well as the CNMF-E algorithm, which dramatically reduces memory and computation requirements. (Plus more topics!)
More upcoming talks for our February multiphoton imaging workshop (Applications OPEN!) https://t.co/CHjMcQ9RZO
Jerome Lecoq @LecoqJerome – "Running a two-photon calcium imaging segmentation pipeline with built-in deep learning denoising at scale"
Darcy Peterka - "Multiphoton tools for exploring and controlling neural circuits"
The multiphoton toolkit has expanded immensely in the last decade. I'll describe some modern tools for imaging & control, with an eye towards considerations for using these tools effectively.
Highlighting some software and data sections for our upcoming workshop!
Peter Rupprecht: Interpretation of calcium imaging data: post-processing, spike inference and artifacts
Kushal Kolar: New mesmerize library helps w/data organization & parameter optimization for calcium imaging analysis https://t.co/QrMe7T17ZA
"fastplotlib is a new visualization library using bleeding edge graphics technology https://t.co/24zSQ1zA5D"
Call for papers for a special section on Understanding of Neural Circuits with Neurophotonics is open now! Guest editors include nemonic's own Spencer Smith
@Labrigger https://t.co/wylRNUdCNb
We are very excited with our group of speakers for this year's workshop! This is a hands-on workshop, where you will run imaging analysis software and see instrumentation demonstrations. https://t.co/OXQAnTxnc0
Apply now to the 2023 Nemonic Workshop! This event will be held Feb 21-23, 2023 in Santa Barbara https://t.co/OXQAnTxnc0. Hear from experts and see demonstrations on multiphoton imaging instrumentation and analysis. Past material: https://t.co/CHjMcPSOXO
Come to the 2023 Nemonic Workshop. https://t.co/C9zEu8zbK9 Feb 21-23, 2023 in Santa Barbara, California. @UNC_Optics@ptrrupprecht@LecoqJerome and others. Come learn about the cutting edge of multiphoton (2p,3p) imaging in neuroscience, both instrumentation and analysis. (1/2)