Oxygenly

Northeastern University Chemical Engineering Capstone Team. Designing a fine control hypoxic chamber for cell culturing experiments.

[Oxygenly @OxygenlyLLC]

@jabulencia @NU_ChemE N2 is added as a pulse injection (~5psi stream) in combination with an active relief valve opening. That's a great point about potentially still affecting cells when doing this even w/out any pressurization & something we should look into more to verify if effects are negligible

[Oxygenly @OxygenlyLLC]

The Stem-Nest: A Controllable Hypoxic Chamber For Cell Culture Experiments. Designed by Casey Kuehnapfel, Doug Pecze, Kelsey Walak, Lisa Sherman, and Yazid Al-Khatib. Check out our poster and infomercial!

Informercial: https://t.co/PbNw2OELQ8

#CHME4703Poster
@NU_ChemE

[Oxygenly @OxygenlyLLC]

@WillitsLab @NU_ChemE We have designed our product as an alternative to disposable chambers and it is constructed out of polycarbonate, which is strong and durable and can be recycled at the end of its lifetime. Also, the market analysis is coming soon in our final report!

[Oxygenly @OxygenlyLLC]

@jabulencia @NU_ChemE Thank you, we're glad you enjoyed our project! Our device does not require reformulation and should function with any standard cell culture media. Our next steps also include better characterizing dissolved oxygen concentration in media over time in conjunction with our device

[Oxygenly @OxygenlyLLC]

@CamelinaSativa @NU_ChemE Very cool project. I see that the plant will make $120 million in gross sales, but have you seen if the plant is profitable in the end?

[Oxygenly @OxygenlyLLC]

@Celluol_21 @NU_ChemE Hi Celluol! Great poster! You mentioned in next steps you're looking to decrease costs while maintaining purity through a variety of potential measures (smaller scale, different enzymes). Is the current process profitable? How much do you anticipate needing to cut costs?

[Oxygenly @OxygenlyLLC]

@donepegel @NU_ChemE Making a dynamic control system that would work with varying adjustable sizes is an interesting idea. Should the market show a need for such flexibility, Oxygenly would further explore this idea’s potential.

[Oxygenly @OxygenlyLLC]

@donepegel @NU_ChemE The current chamber size is 15”x11”x5”, which would fit several cell-cultures, depending on their size. For larger implementations, the Stem-Nest could be scaled up by calibrating the process control system (nitrogen input vs. oxygen reintroduction) to the new dimensions.

[Oxygenly @OxygenlyLLC]

@donepegel @NU_ChemE We’re excited by your interest! Yes, the Stem-Nest is designed to be placed in an incubator, as besides providing a 37C environment, it will also be the source of humidity, carbon dioxide and oxygen through our micro air pump valve.

[Oxygenly @OxygenlyLLC]

@Profess88700345 @NU_ChemE Hi Team 13, poster looks great! From figure 2, why do the small chain PFAS compounds have the longest residence time? It seems longer chain PFAS compounds would take longer to break down. Do the longer chain compounds transform into the smaller chain compounds?

[Oxygenly @OxygenlyLLC]

@AkhilAgaku @dennayyyyy @renegold8 @GabyBaezG @JJForsterIII Hi Team 12! Very nice poster and cool idea. I know the Haber-Bosch process is responsible for a little less then half of all the nitrogen fixation on the planet. Could your process potentially completely replace H-B? Will the resources (space, waste, etc.) allow it?

[Oxygenly @OxygenlyLLC]

@BiotechVert @NU_ChemE Any cells kept in a traditional incubator must be removed to be worked on, similar to our device; brief exposure to ambient conditions is generally not considered detrimental. Otherwise an incubating hypoxic glove box is required which can cost $20000+, money many labs don't have

[Oxygenly @OxygenlyLLC]

@BiotechVert @NU_ChemE Yes the chamber will lose its hypoxic state when the cells are being worked on. That is just a condition of the device. The cells will be okay with the short exposure to high oxygen and the atmosphere will be quickly re-established once the cells are returned to the chamber!

[Oxygenly @OxygenlyLLC]

@BiotechVert @NU_ChemE Hypoxic conditions are created by flushing the chamber with nitrogen. Our chamber has both an active relief and passive relief to prevent pressurization. The incubators our device will be used in have venting capabilities since the systems uses compressed CO2 already.

[Oxygenly @OxygenlyLLC]

@BiotechVert @NU_ChemE 5% oxygen is optimal for neural stem cells since that is their physiological oxygen concentration. Our device can hold a set point anywhere from 0% to atmospheric oxygen concentration. You can set the oxygen set point using the buttons on our electronic box!

[Oxygenly @OxygenlyLLC]

@BiotechVert @NU_ChemE Hi Vert Biotech, we have not run dissolved oxygen measurements yet but that is our next planned experiment! We will be running the experiment inside the chamber, inside an incubator. The proof of concept on the poster is just atmospheric.