PhageCast

[PhageCast @PhageCast]

Not all bacterial cells in a biofilm are equally susceptible to phages. What factors drive phage activity and how can phages adapt to target different bacterial subpopulations?

We discuss this, the implications for phage therapy and much more!

Enjoy the episode!

[PhageCast @PhageCast]

New Phage Bites episode!

We speak with @lperestrelom about her recent Nature Communications study on the directed evolution of phages in biofilms and how this approach can improve the control of Pseudomonas aeruginosa.

https://t.co/G1DFC4yIdd

[PhageCast @PhageCast]

Only a fraction of bioinformatically predicted prophages were inducible under laboratory conditions.

What explains the discrepancy between prediction and experimental observation?

Listen to:
https://t.co/sF8GrGnePH

[PhageCast @PhageCast]

What does the publication process look like for a Nature paper?

We discuss the evolution of the manuscript through peer review and lessons from an extended revision process.

If you’re curious, listen here:
https://t.co/sF8GrGnePH

[PhageCast @PhageCast]

Which conditions trigger prophage induction in gut bacteria?

We discuss experimental observations across multiple environmental contexts and their implications for understanding virome activity in situ.

🎧 https://t.co/sF8GrGnePH

[PhageCast @PhageCast]

A proof-of-principle framework for experimentally studying temperate phages from the human gut, integrating isolation, induction assays, and ecological analysis.

Discussed in our last episode with Jeremy Barr.
🎧 https://t.co/sF8GrGnePH

[PhageCast @PhageCast]

Lytic vs. lysogenic cycles, temperate vs. virulent phages, prophages, lysogens… terminology can be confusing, even within the field.

We clarify these concepts in the context of gut phage ecology.

Listen here:
https://t.co/sF8GrGnePH

[PhageCast @PhageCast]

Much of what we know about the gut virome comes from metagenomics.

But what do these viruses actually do in the microbiome?

We discuss experimental approaches to studying temperate phage activity in the human gut with Prof Jeremy Barr.

Listen here:
https://t.co/sF8GrGnePH

[PhageCast @PhageCast]

We also speak about the process of publishing in Nature and much much more in the episode.

Enjoy the episode 🎧

[PhageCast @PhageCast]

New episode out!
In this new episode we are so excited to share our interview with Prof. @JeremyJBarr (again!)

We speak about his innovative approach on studying the gut virome, tune in if you wanna hear the phage study that made it into Nature.

[PhageCast @PhageCast]

We speak about environmental factors that may influence prophage induction from lysogens in the gut, how dietary compounds or epithelial perturbation during inflammation can shape gut virome dynamics.
🧵

[PhageCast @PhageCast]

One of the most surprising findings:

How is Acinetobacter connected to Crohn’s disease and phages?

We discuss this in the latest Phagecast episode.
Stay until the end:

https://t.co/yAYxDjMMaO

[PhageCast @PhageCast]

Do all bacteriophages cross the gut barrier equally?

“There are several barriers from the gut: epithelium, endothelium…

Tune in our last episode to hear more about it!

https://t.co/yAYxDjMMaO

[PhageCast @PhageCast]

In Crohn’s disease, the intestinal barrier is damaged. As Luisa explains “We look at translocation because the gut is hyperpermeable and over-inflamed.” What do you think happens with gut viruses then?

Tune in to hear the answer!

https://t.co/yAYxDjMMaO

[PhageCast @PhageCast]

In our last episode, we interview @luisadesordi and talk about how bacteriophages shape inflammation and may even cross the intestinal barrier in Crohn’s disease to get to the blood.

Tune in to hear the new episode!

https://t.co/yAYxDjMMaO

[PhageCast @PhageCast]

Throughout the conversation, we focus on the biological mechanisms underlying phage translocation and what these findings suggest about the gut–blood virome axis in inflammatory disease.

We also asked some personal questions and got some valuable advice!
Enjoy the episode!

[PhageCast @PhageCast]

In this episode, we speak with @luisadesordi (Sorbonne University) about her research on gut phages and their role in Crohn’s disease. We discuss whether phages can cross the intestinal barrier into the bloodstream and how this differs in healthy individuals vs. Crohn’s patients.

[PhageCast @PhageCast]

The study shows that certain phages translocate at higher levels in Crohn’s patients. We also cover the experimental approach used to track phages across epithelial layers, including fluorescent labeling and other methods and questions they answered!