PlesaLab

Official lab news from the Plesa Lab at @UOKnightCampus

[Calin Plesa @calinplesa]

We just preprinted DropSynth-Gold where we replaced PCA with Golden Gate Assembly (GGA) inside emulsion droplets, extending multiplexed gene synthesis to ~3 kb constructs assembled from up to 12 fragments while preserving the scalability of DropSynth. 1/n

[Calin Plesa @calinplesa]

New lab preprint: if ML struggles with extrapolation, let's expand the diversity of training data with gene synth, DNA shuffling, and ML gen.... also lots of #FluorescentProteins #ProteinEngineering #MachineLearning #SyntheticBiology 1/n

[Calin Plesa @calinplesa]

Need long-read sequencing? We’ve just completed the first ever run on @uoregon GC3F core’s new @PacBio Revio instrument! Perfect for amplicons too long for Illumina, and with Kinnex prep, you can get >10x more data. Great pricing, no queue, try it out. https://t.co/JX8fItNENY

[PlesaLab @PlesaLab]

Lab memes!

[Calin Plesa @calinplesa]

Congratulations @TrieneToScience for winning the people’s choice award at the Oregon Bio Innovation Showcase today!!

[PlesaLab @PlesaLab]

Interested in antibody generation and expression of human proteins in bacteria? If you're at @ProteinSociety tonight check out poster 75 at Bayshore Grand Ballroom D with @MuggleInSynbio to hear about expressing the human proteome in E. coli. @UOKnightCampus

[Knight Campus @UOKnightCampus]

Powerful 1st-person piece by Knight Campus Dept of Bioengineering Asst Prof Parisa Hosseinzadeh on her long road to recovery following a brain hemorrhage in Nov. 2022. We are so inspired by your strength & determination Parisa and are happy to see you growing stronger every day

[PlesaLab @PlesaLab]

At @ProteinSociety tonight check out poster 73 with @AlkynesOfSci to hear about our efforts to deorphanize sensor histidine kinases en masse & poster 117 with @TrieneToScience to hear about BAR-CAT, our approach to scalable enrichment of assembled gene libraries. @UOKnightCampus

[Sayandeep @MuggleInSynbio]

Christmas came early in @PlesaLab with the latest Degenerate DropSynth preprint and the fun was doubled up by lovely Secret Santas in the lab and the Impact & Innovation Award in the annual celebration at @UOKnightCampus 🎉🎁
Signing off 2023 slowly with style 😎

[Calin Plesa @calinplesa]

Story time! Introducing the first of several new flavors of DropSynth #genesynthesis we have been working on, cooked up by @AlkynesOfSci and Sam Hinton. TLDR: We show multiple gene variants assembled per barcoded droplet and 1 kbp lengths. 1/n
https://t.co/8n8qiQA1ik

[Knight Campus @UOKnightCampus]

The Department of Bioengineering Seminar Series continued today with Biochemistry PhD student Andrew Holston (Plesa Lab) and #KnightCampusBioe PhD student Sam Lester (Guldberg Lab) who gave talks in the Beetham Seminar Room. The seminar series is held most Mondays.

[Knight Campus @UOKnightCampus]

Roughly half the world's population is infected with H. pylori, a leading cause of stomach cancer. A group of UO undergraduates are working on a probiotic they hope could be a gamechanger. The iGEM team is raising funds to take their project to Paris: https://t.co/p49wmAeiaD

[Natanya Villegas @TrieneToScience]

Carmen, Yuki, and I representing @PlesaLab from @UOKnightCampus at #VariantEffect23 ! It's been a pleasure to see the amazing research and to share our work.

[Natanya Villegas @TrieneToScience]

@PlesaLab is full of cool surprises!

[Sayandeep @MuggleInSynbio]

When you hit the desk on a weekend to spot an art by the #LOTR geek, and instantly know it’s none other than your PI ! @PlesaLab

How many of you have items named in the lab?

#research #sciencetwitter #academiclife

[Sayandeep @MuggleInSynbio]

A perfect getaway for a lab retreat on a sunny weekend.
More to follow soon!
@PlesaLab @sweetcheekswine

[Sayandeep @MuggleInSynbio]

End of the year celebration in @UOKnightCampus and what a blast ❤️❤️❤️

[PlesaLab @PlesaLab]

A perfect 20.000 score on the Nanophotometer
Attracts attention, but watch those ratios, or else you'll be a goner!
Stay focused, don't let your measurements falter. 9/n

[PlesaLab @PlesaLab]

Just in time for Winter a quick tour of the more unusual things around our lab... 1/n

[PlesaLab @PlesaLab]

Forgotten buffers have been colonized,
By herds of wooly mammoths, a surprise, oh my goodness gracious!
It's a sight to behold, these majestic creatures on the scene,
In the forgotten buffers, they now reign supreme. 8/n