RevoluGen

[RevoluGen @RevoluGen]

NEWS We are absolutely delighted to announce our agreement with #ProteoQuest for the distribution of Fire Monkey HMW-DNA extraction kits across #China including Hong Kong and Taiwan. User-friendly, single extraction for both @illumina @nanopore reads.

https://t.co/kwxFqCbZHn

[RevoluGen @RevoluGen]

Low Molecular Weight mammalian DNA input ➡️Fire Monkey beads size selection/fragmentation workflow ➡️appx. 30Gb on a single LSK114/MinION run @nanopore

[RevoluGen @RevoluGen]

Single extraction/fragmentation protocol (magnetic beads) => single (no nuclease flushes) mammalian genome run. 21kb and 22Gb on a MinION flow cell, LSK114 @nanopore

[RevoluGen @RevoluGen]

White blood cells extraction/fragmentation magnetic beads protocol. 460+ pores sequencing, N50:21kb @nanopore LSK114/MinION.

[RevoluGen @RevoluGen]

Fully automated Kingfisher @thermosci Fire Monkey beads HMW-DNA extraction from white blood cells. No size selection post-extraction LSK114/MinION @nanopore

[RevoluGen @RevoluGen]

In development: Low molecular weight DNA extract input fragmentation on Fire Monkey magnetic beads for long @nanopore reads

[RevoluGen @RevoluGen]

Fire Monkey chemistry + Dynabeads/Kingfisher Apex @thermofisher. 1ml E coli culture, on deck Proteinase K lysis to elution automated workflow. Raw data : 14Gb, read N50: 27kb on MinION/LSK114 @nanopore, single run.

[SPTLabtech @SPTLabtech]

We've collaborated with @RevoluGen to deliver a fully automated protocol that produces HMW DNA for Nanopore sequencing in under 2 hours, representing a meaningful advance in sample preparation efficiency for long-read #DNAsequencing:

https://t.co/jVA88RDboW

#genomics

[RevoluGen @RevoluGen]

Results of collaboration with @LGCGroup demonstrating automated HMW-DNA extraction combining Fire Monkey chemistry + LGC magnetic beads + oKtopure liquid handling robot = superior HMW-DNA for short & long read #sequencing
https://t.co/0pPiGCLE9d

[RevoluGen @RevoluGen]

Breakthrough bead-based automated, #HMWDNA extractions for high throughput long reads. #oKtopure instrument using Fire Monkey chemistry & @LGCGroup magnetic beads generates 21Gb of raw data, N50 ~45kb on @nanopore LSK114/MinION without post-extraction size selection. #Sequencing

[RevoluGen @RevoluGen]

In development. Miniaturized and automatable. Beads fragmentation of low and high molecular weight DNA input @nanopore @PacBio @illumina

[RevoluGen @RevoluGen]

Fire Monkey magnetic beads for mammalian samples. HMW-DNA or tuneable, miniaturized and automatable. No resting, fragmentation or size selection post-extraction. LSK114/MinION @nanopore performance.

[RevoluGen @RevoluGen]

Miniaturized HMW-DNA extractions: 200microLt E coli culture + reduced Fire Monkey solution volumes on magnetic beads -> MinION/LSK114 @nanopore

[RevoluGen @RevoluGen]

E coli Fire Monkey magnetic beads HMW-DNA extract captured on a precipitation star @nanopore

[RevoluGen @RevoluGen]

@YMLEE3993869750 @nanopore Thank you! We will keep in mind for the next run.

[RevoluGen @RevoluGen]

100microLt whole blood, Fire Monkey magnetic, no size-selection post extraction, MinION LSKv14 @nanopore => ~8Gb and N50:40kb after 17hrs.

[RevoluGen @RevoluGen]

@YMLEE3993869750 @nanopore Fast. A few more tweaks remaining before full run with nuclease washes.

[RevoluGen @RevoluGen]

@biodiversityge1 @AlexKneubehl @nanopore Dear Matthew. We have not tried fish. This might require tissue homogenisation and longer treatments. Also, we use a precipitation step for whole blood that might help. For more details please contact [email protected]

[RevoluGen @RevoluGen]

Fire Monkey magnetic beads extract + Ligation Sequencing Kit V14/MinION @nanopore

[RevoluGen @RevoluGen]

@AlexKneubehl @nanopore Dear Alex, we don't support this sample type at the moment.