NEWS We are absolutely delighted to announce our agreement with #ProteoQuest for the distribution of Fire Monkey HMW-DNA extraction kits across #China including Hong Kong and Taiwan. User-friendly, single extraction for both @illumina@nanopore reads.
https://t.co/kwxFqCbZHn
Low Molecular Weight mammalian DNA input ➡️Fire Monkey beads size selection/fragmentation workflow ➡️appx. 30Gb on a single LSK114/MinION run @nanopore
Single extraction/fragmentation protocol (magnetic beads) => single (no nuclease flushes) mammalian genome run. 21kb and 22Gb on a MinION flow cell, LSK114 @nanopore
Fully automated Kingfisher @thermosci Fire Monkey beads HMW-DNA extraction from white blood cells. No size selection post-extraction LSK114/MinION @nanopore
Fire Monkey chemistry + Dynabeads/Kingfisher Apex @thermofisher. 1ml E coli culture, on deck Proteinase K lysis to elution automated workflow. Raw data : 14Gb, read N50: 27kb on MinION/LSK114 @nanopore, single run.
We've collaborated with @RevoluGen to deliver a fully automated protocol that produces HMW DNA for Nanopore sequencing in under 2 hours, representing a meaningful advance in sample preparation efficiency for long-read #DNAsequencing:
https://t.co/jVA88RDboW
#genomics
Results of collaboration with @LGCGroup demonstrating automated HMW-DNA extraction combining Fire Monkey chemistry + LGC magnetic beads + oKtopure liquid handling robot = superior HMW-DNA for short & long read #sequencing
https://t.co/0pPiGCLE9d
Breakthrough bead-based automated, #HMWDNA extractions for high throughput long reads. #oKtopure instrument using Fire Monkey chemistry & @LGCGroup magnetic beads generates 21Gb of raw data, N50 ~45kb on @nanopore LSK114/MinION without post-extraction size selection. #Sequencing
Fire Monkey magnetic beads for mammalian samples. HMW-DNA or tuneable, miniaturized and automatable. No resting, fragmentation or size selection post-extraction. LSK114/MinION @nanopore performance.
@biodiversityge1@AlexKneubehl@nanopore Dear Matthew. We have not tried fish. This might require tissue homogenisation and longer treatments. Also, we use a precipitation step for whole blood that might help. For more details please contact [email protected]