Sebastian Lux

Uni Leipzig, Bioanalytics

[Sebastian Lux @SebastianSchmu6]

#Fructose #MaillardReaction #GlycatedPeptides #AmadoriHeynsProducts (6)

[Sebastian Lux @SebastianSchmu6]

Amadori and Heyns products: Same mass, similar retention – but so different in shape!

In our latest research, published open access in @AnalBioanalChem, we took further steps towards the comprehensive analysis of the fructated proteome.

Full article:
▶️https://t.co/MRLizq7hrU

[Sebastian Lux @SebastianSchmu6]

"Limitations of boronate affinity chromatography for the specific enrichment of fructose-derived early glycation products in protein analytics". Lux S, Vogt C, Voll M, Hoffmann R. Anal Bioanal Chem (2025). (5)

[Wiley Analytical Science @WileyAnalytical]

Separation of glycation isomers

Read the full article:
▶ https://t.co/oZDYxnOAP2

#Proteomics #Fructose #Diabetes #WileyAnalyticalScience #RP-HPLC

[Sebastian Lux @SebastianSchmu6]

I would like to thank the editors for considering our work and giving it a platform to reach a wider audience. I highly enjoyed the collaboration and appreciate their contribution to this article.

[Sebastian Lux @SebastianSchmu6]

Delighted to see that our research has been featured in the latest HPLC special issue of GIT Labor-Fachzeitschrift [04/2023], which focuses on bioanalytics.

https://t.co/UkbP1B44TM

#gitlabor, Wiley Analytical Science, #science, #separation, #spektroskopie, #HPLC, #Bioanalytik

[Sebastian Lux @SebastianSchmu6]

Last but not least - the final chapter of my thesis is finally published in @AnalBioanalChem and available open access. This time, we studied the separation of isomeric early glycation products formed by the reaction of peptides with fructose or glucose using RP-HPLC and HILIC.

[Kermit Murray @kermitmurray]

Chromatographic separation of glycated peptide isomers derived from glucose and fructose https://t.co/t1MKMGdkY5 #MassSpecPub #AnalBioanalChem

[Your HPLC DataBase @LC_Analysis]

Differentiation and Quantitation of Coeluting Isomeric Amadori and Heyns Peptides Using Sugar-Specific Fragment Ion Ratios https://t.co/rHxLZHitgk

[Sebastian Lux @SebastianSchmu6]

The method was validated for digests of fructated and glucated recombinant HSA and finally used to analyze human plasma digests spiked with isotope labeled Amadori-peptides and enriched for glycation products. (6)

[Sebastian Lux @SebastianSchmu6]

Excited and proud to see our work finally published in analytical chemistry. A big thanks to my coauthors @MicheleWoelk and Ralf Hoffmann, and supporter @FedorovaLab!

[BioMassSpec @realBioMassSpec]

Differentiation and Quantitation of Coeluting Isomeric Amadori and Heyns Peptides Using Sugar-Specific Fragment Ion Ratios #AC https://t.co/xq0VjQBKI1

[Sebastian Lux @SebastianSchmu6]

Quantification of peptides was performed using non-glycated y- and b-ions, which were equally formed by both isomers. (5)