First publication is finally online ๐๐! Here is a video description from lead author, Professor Paul McMenamin, explaining the distribution of melanoblasts in the early embryonic mouse choroid @SarahCoupland3@KaliraiHelen@ARVOiovs#LOORG#Melanoblast#Melanocyte
First publication is finally online ๐๐! Here is a video description from lead author, Professor Paul McMenamin, explaining the distribution of melanoblasts in the early embryonic mouse choroid @SarahCoupland3@KaliraiHelen@ARVOiovs#LOORG#Melanoblast#Melanocyte
Melanocytes populate the mouse choroid earlier in development than previously described | IOVS | ARVO Journals https://t.co/ETdVYdN0OI. Our ARVO Abstract is now published: hoping to get full article revised & resubmitted soon! @icanceresearch@UMCURE2020
@CRaamsdonk Thanks very much for your interest Catherine, the images you shared look great! @SarahCoupland3, @KaliraiHelen and I follow your work closely and have read your article. Your findings and mouse models are very interesting.
Thanks to everyone who showed interest in my poster for #HLSPosterDay2020! For anyone interested to know more the related abstract will be published next month in
@ARVOiovs, and we are currently waiting to hear back about the submission status of our finished paper #LOORG
@plant_DOM @SarahCoupland3@KaliraiHelen@icanceresearch @LivHospitals Thank you! Currently we haven't discussed on whether to conduct experiments of this nature or not. But historically we do know from conditions such as Type 2 Waardenburg syndrome, that the absence of melanocytes in the eyes and other areas can be detrimental to normal development
@RoyGoodacre @SarahCoupland3@KaliraiHelen@icanceresearch @LivHospitals Thanks Roy! We've got a few different areas we are investigating, but I think the main one right now is determining the route of migration - both from the neural crest and into the eye. Thankfully I've still got plenty of time with my PhD to explore the possibilities
@LippyLiptrott@SarahCoupland3@KaliraiHelen@icanceresearch @LivHospitals However, I am very interested in looking at this in more detail. By looking for these other markers we could identify the age at which the cells differentiate, which to my knowledge, has not been described either
@LippyLiptrott@SarahCoupland3@KaliraiHelen@icanceresearch @LivHospitals At the beginning of this project one of my collaborators, Professor Paul McMenamin, tested a variety of these markers on the same species/strain and found many did not stain as clearly or reliably. Since I've joined we haven't investigated this further
@LippyLiptrott@SarahCoupland3@KaliraiHelen@icanceresearch @LivHospitals To expand a bit further, TRP2 is expressed in the melanosome membrane (the pigment producing organelle in melanocytes). The melanosomes have 4 stages of development themselves, but TRP2 is expressed throughout all of them. This allowed us to detect the early differentiating cells
@LippyLiptrott@SarahCoupland3@KaliraiHelen@icanceresearch @LivHospitals Hello Neill, I think it's mainly to do with the marker we used (TRP2) and the time points we looked at. I've found when researchers have looked at embryonic eyes previously its either with a marker expressed later in melanoblast/melanocyte development or at an age later than E15.
A better understanding of early choroidal embryology is very important for novel insights into eye cancer. Our research seeks to better comprehend choroidal melanocytes and why they undergo malignant change into melanomas @UMCURE2020#UvealMelanoma