Graham Shields

PhD student | Liverpool Ocular Oncology Research Group | University of Liverpool | Focusing on choroidal melanocyte development.

[Graham Shields @Shields_GT]

First publication is finally online 😄🎉! Here is a video description from lead author, Professor Paul McMenamin, explaining the distribution of melanoblasts in the early embryonic mouse choroid @SarahCoupland3 @KaliraiHelen @ARVOiovs #LOORG #Melanoblast #Melanocyte

[Sarah Coupland 💙🔬🚴🏻‍♂️📷 🇺🇦 @SarahCoupland3]

Melanoblasts Populate the Mouse Choroid Earlier in Development Than Previously Described | IOVS | ARVO Journals: ⁦@icanceresearch⁩ ⁦@UMCURE2020⁩ ⁦@LivUniISMIB⁩ ⁦@MelanomaUK⁩ ⁦@focusonmelanoma⁩ https://t.co/tI8OEK5w0J

[LOORG @icanceresearch]

LOORG GREAT WELSH WALK 2021 - Liverpool Ocular OncologyResearch Group https://t.co/Z8njhPyNTH

@sarahcoupland3
@LivUni
@unilever

https://t.co/SWOiKo2hGf

[Graham Shields @Shields_GT]

@lunadjirackor @icanceresearch @SarahCoupland3 @KaliraiHelen @ARVOiovs Thank you so much Luna 😁!

[Graham Shields @Shields_GT]

First publication is finally online 😄🎉! Here is a video description from lead author, Professor Paul McMenamin, explaining the distribution of melanoblasts in the early embryonic mouse choroid @SarahCoupland3 @KaliraiHelen @ARVOiovs #LOORG #Melanoblast #Melanocyte

[Sarah Coupland 💙🔬🚴🏻‍♂️📷 🇺🇦 @SarahCoupland3]

Melanoblasts Populate the Mouse Choroid Earlier in Development Than Previously Described | IOVS | ARVO Journals: ⁦@icanceresearch⁩ ⁦@UMCURE2020⁩ ⁦@LivUniISMIB⁩ ⁦@MelanomaUK⁩ ⁦@focusonmelanoma⁩ https://t.co/tI8OEK5w0J

[Graham Shields @Shields_GT]

@robinsall Thanks Robert!

[Sarah Coupland 💙🔬🚴🏻‍♂️📷 🇺🇦 @SarahCoupland3]

Melanocytes populate the mouse choroid earlier in development than previously described | IOVS | ARVO Journals https://t.co/ETdVYdN0OI. Our ARVO Abstract is now published: hoping to get full article revised & resubmitted soon! @icanceresearch @UMCURE2020

[Graham Shields @Shields_GT]

@CRaamsdonk Thanks very much for your interest Catherine, the images you shared look great! @SarahCoupland3, @KaliraiHelen and I follow your work closely and have read your article. Your findings and mouse models are very interesting.

[Graham Shields @Shields_GT]

Thanks to everyone who showed interest in my poster for #HLSPosterDay2020! For anyone interested to know more the related abstract will be published next month in
@ARVOiovs, and we are currently waiting to hear back about the submission status of our finished paper #LOORG

[Graham Shields @Shields_GT]

@elinorchapman @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals Thank you😁!

[Graham Shields @Shields_GT]

Very excited to share our research for #HLSPosterDay2020, which was also lined up for #ARVO2020. We show that choroidal melanoblasts are present in the mouse choroid, much earlier than previously thought! @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals #LOORG #HLSSMIB

[Graham Shields @Shields_GT]

@raphavisses Thank you for your comments and sharing! I'll make sure to note this the next time I am presenting this work 😁

[Graham Shields @Shields_GT]

@plant_DOM @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals I think so too, thanks!

[Graham Shields @Shields_GT]

@plant_DOM @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals Thank you! Currently we haven't discussed on whether to conduct experiments of this nature or not. But historically we do know from conditions such as Type 2 Waardenburg syndrome, that the absence of melanocytes in the eyes and other areas can be detrimental to normal development

[Graham Shields @Shields_GT]

@RoyGoodacre @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals Thank you!

[Graham Shields @Shields_GT]

@RoyGoodacre @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals Thanks Roy! We've got a few different areas we are investigating, but I think the main one right now is determining the route of migration - both from the neural crest and into the eye. Thankfully I've still got plenty of time with my PhD to explore the possibilities

[Graham Shields @Shields_GT]

@LippyLiptrott @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals No problem! Thanks for the interest in my research😁

[Graham Shields @Shields_GT]

@LippyLiptrott @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals However, I am very interested in looking at this in more detail. By looking for these other markers we could identify the age at which the cells differentiate, which to my knowledge, has not been described either

[Graham Shields @Shields_GT]

@LippyLiptrott @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals At the beginning of this project one of my collaborators, Professor Paul McMenamin, tested a variety of these markers on the same species/strain and found many did not stain as clearly or reliably. Since I've joined we haven't investigated this further

[Graham Shields @Shields_GT]

@LippyLiptrott @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals To expand a bit further, TRP2 is expressed in the melanosome membrane (the pigment producing organelle in melanocytes). The melanosomes have 4 stages of development themselves, but TRP2 is expressed throughout all of them. This allowed us to detect the early differentiating cells

[Graham Shields @Shields_GT]

@LippyLiptrott @SarahCoupland3 @KaliraiHelen @icanceresearch @LivHospitals Hello Neill, I think it's mainly to do with the marker we used (TRP2) and the time points we looked at. I've found when researchers have looked at embryonic eyes previously its either with a marker expressed later in melanoblast/melanocyte development or at an age later than E15.

[Graham Shields @Shields_GT]

A better understanding of early choroidal embryology is very important for novel insights into eye cancer. Our research seeks to better comprehend choroidal melanocytes and why they undergo malignant change into melanomas @UMCURE2020 #UvealMelanoma