Sternberg Lab

News from the Sternberg lab at Columbia Univ., HHMI. Posts are from lab members and not Samuel Sternberg unless signed SHS. Posts represent personal views only.

[Sternberg Lab @SternbergLab]

7/7 As a lab we believe in sharing both our science and our research knowledge with others, and hope we’ve inspired a few minds! Looking forward to the next visit!

[Sternberg Lab @SternbergLab]

1/7 Last week the Sternberg Lab welcomed ~40 junior & senior high school AP Research students from the East Meadow school district (and their incredible teachers) into our lab at Columbia University.

[Sternberg Lab @SternbergLab]

6/7 And they enjoyed this agarose gel meme, made especially for our young friends ;)

[Sternberg Lab @SternbergLab]

5/5 Huge congratulations to all the authors for their incredible work bringing this story together! It was a fantastic collaborative effort spanning time zones and continents.

[Sternberg Lab @SternbergLab]

1/5 Telomerase has long been linked to chromosome maintenance in eukaryotes. Now, we show that telomerase-like activity is also present in bacteria, shedding new light on its ancient origins.

Excited to share that our work on DRT10 is now out in @Nature!

https://t.co/4CekquyMXX

[Sternberg Lab @SternbergLab]

4/5 DRT10 biochemistry and structure (with @IsraelF96135088): https://t.co/xRYmmVVFiF

[Sternberg Lab @SternbergLab]

1/11 New preprint from the Sternberg lab in collaboration with the Fernández lab! We are excited to share our structure-function study of DRT10, a bacterial defense-associated reverse transcriptase that synthesizes long tandem-repeat DNA.🧵
More here: https://t.co/6eukESpDSz

[Sternberg Lab @SternbergLab]

2/2 We used sequencing and fluorescence reporter assays to uncover Arc’s role as a transcriptional repressor. It binds the transposon promoter and regulates gene expression, suggesting a new mechanism for transposon control!

[Sternberg Lab @SternbergLab]

1/2 Here we report the activity of Arc, a 50-aa DNA-binding protein encoded within IS605-family transposons. https://t.co/dlRJPViJr1

[Sternberg Lab @SternbergLab]

13/13 — A joint effort between the Sternberg and Chang labs: Renjian Xiao, Americo Casas-Ciniglio, Henry Le, Tanner Wiegand, and Dan Xie, supervised by Leifu Chang and Sam Sternberg.

[Sternberg Lab @SternbergLab]

1/13 — New pre-print from the Sternberg Lab in collaboration with Leifu Chang's Lab! TnpB nucleases have repeatedly given rise to TldRs, nuclease-dead RNA-guided transcription factors. Here we define the biological role of a TldR clade in its native host.
https://t.co/NkVXPW0VHb

[Sternberg Lab @SternbergLab]

12/13 — TldR is a naturally evolved parallel to engineered CRISPRi. Repression of an OppA variant shifts the relative contribution of remaining paralogs, which could influence which peptides a cell imports. What induces TldR natively remains unknown.
https://t.co/NkVXPW1twJ