Geoff Wahl Lab

Cancer Biologists @salkinstitute, single cell enthusiasts, working to end breast and pancreas cancers

[Kathy DelGiorno @DelgiornoKathy]

Official Ad! Please join my laboratory!
https://t.co/8eFPXq3H1d

[Kathy DelGiorno @DelgiornoKathy]

Our recent @AGA_Gastro article was just highlighted in @NatRevGastroHep https://t.co/BcSd62YxHI

[Geoff Wahl Lab @WahlLabSalk]

Another recent paper from Wahl lab on tuft cell formation in pancreatic injury! Check this out! Great job done by @RaziaNaeem and @DelgiornoKathy

[Frontiers - Physiology @FrontPhysiol]

New Research: Tuft Cell Formation Reflects Epithelial Plasticity in Pancreatic Injury: Implications for Modeling Human Pancreatitis https://t.co/O8MtKu8PUv #Physiology

[Geoff Wahl Lab @WahlLabSalk]

Understanding such mechanisms may help to identify therapeutic avenues to promote targeted depletion of RAS proteins, such improvements could greatly benefit a large number of patients with pancreatic and other cancers that depend on oncogenic activation of RAS proteins.

[Geoff Wahl Lab @WahlLabSalk]

The Wahl lab @salkinstitute discovered a new mechanism for regulating #RAS activity and protein levels, which provides a novel therapeutic strategy for inhibiting and depleting oncogenic RAS proteins! Great work by @yaochengli and colleagues! @PNASNews https://t.co/rcFYE9AZwl

[Geoff Wahl Lab @WahlLabSalk]

5. High-affinity conventional PPI between RAS and RAS binders cause inhibition, which is mediated by an autophagosome-lysosome degradation pathway. Membrane targeted strong RAS binding proteins deplete K-, N-, and H-RAS proteins (check the details in the paper).

[Geoff Wahl Lab @WahlLabSalk]

@geoffrey_wahl @yaochengli @NikkiLytle @DelgiornoKathy @ZhiboM

[Geoff Wahl Lab @WahlLabSalk]

4. MAFI-mediated RAS-RBD_R89L low-affinity interaction is not sufficient to inhibit RAS activity. Rather, high-affinity classic PPI between RAS and RBD is required to inhibit ERK phosphorylation. (p.s. Look his beautiful western blots!)

[Geoff Wahl Lab @WahlLabSalk]

3. RAS-RBD interaction was utilized to model conventional protein-protein interaction (PPI) and Membrane Association Facilitated Interaction (MAFI). Both PPI and MAFI could lead to luciferase fusion and activation.

[Geoff Wahl Lab @WahlLabSalk]

2. Different RAS isoforms localize sufficiently close on membrane domains in cells to enable Membrane Association Faccilated Interactions (MAFI) at near-endogenous expression levels in cells.

[Geoff Wahl Lab @WahlLabSalk]

1. To accurately quantify protein-protein interaction, we improved the original split-luciferase assay (ReBiLC) by normalizing the luminescent signal to the amount of expressed 1/2luc fusion proteins. Named it ReBiL2.0!

[Geoffrey Wahl @geoffrey_wahl]

IF you are interested in #breast and/or #pancreas cancer, single cell methods, #development, microscopy, mouse models, etc..WE ARE LOOKING FOR YOU! Hiring Now. @WahlLabSalk

[Geoff Wahl Lab @WahlLabSalk]

@DelgiornoKathy @NikkiLytle @manorlaboratory @sammy_wn @LeoAndrade1974 @_LynnFang @KenOliveLab The Wahl lab and @DelgiornoKathy are now exploring other mechanisms by which TCs inhibit tumorigenesis. Interested? the Wahl lab is hiring!

[Geoff Wahl Lab @WahlLabSalk]

The Wahl lab tackles the enigmatic pancreatic tuft cell! Learn more about how tuft cells restrain cancer from our new preprint at BioRx! @DelgiornoKathy @NikkiLytle @manorlaboratory @sammy_wn @LeoAndrade1974 @_LynnFang @KenOliveLab https://t.co/j7dqRjzrWA

[Geoff Wahl Lab @WahlLabSalk]

@DelgiornoKathy @NikkiLytle @manorlaboratory @sammy_wn @LeoAndrade1974 @_LynnFang @KenOliveLab PGD2 synthase (Hpgds) is also expressed in human pancreas TCs!

[Geoff Wahl Lab @WahlLabSalk]

It's amazing what the @manorlaboratory can do with EM data

[Kathy DelGiorno @DelgiornoKathy]

@sammy_wn is sooo talented. Check out our newest 3-D rendering of a pancreatic tuft cell!