Andrew Smith

Staff Scientist in the @Pagliarini_lab at @WUSTLmed, St. Louis. Big mitochondria fan! Views my own | 🇮🇪 | he/him

[Andrew Smith @AJsmith231]

~90% of mitochondrial proteins have at least one phosphosite.
Only ~1% of these sites have a reported function.

Is this regulation or noise?

We silenced 10 resident mitochondrial phosphatases, one at a time, to find out. New preprint 🧵👇

[Andrew Smith @AJsmith231]

Excited to finally share this story!

Preprint thread here: https://t.co/dmWDZivWe3

[Pagliarini Lab @Pagliarini_Lab]

Is phosphorylation broadly relevant to mitochondrial protein function? In work led by @AJsmith231, we silenced 10 mito phosphatases, mapped dynamic sites, and revealed functional implications for import, catalysis, and morphology.
https://t.co/eeu6BzKL7z
# Mitochondria
#HHMINews

[Andrew Smith @AJsmith231]

Huge thanks to @PatrickForny, Sean, Merima, and @Pagliarini_Lab, and to NIDDK, NIGMS and HHMI. Questions and critiques very welcome!

#mitochondria #phosphoproteomics

[Andrew Smith @AJsmith231]

~90% of mitochondrial proteins have at least one phosphosite.
Only ~1% of these sites have a reported function.

Is this regulation or noise?

We silenced 10 resident mitochondrial phosphatases, one at a time, to find out. New preprint 🧵👇

[Andrew Smith @AJsmith231]

The result: Increased phosphorylation of cristae forming complexes in PGAM5 knockdown cells leads to denser cristae, more MIB assembly, fewer ATP synthase dimers, faster growth, and greater spare respiratory capacity.

Full preprint: https://t.co/sRQqqUEPqb

[Florent Waltz @FlorentWaltz]

Super excited to share our latest work on the native in-cell organization of the mitochondrial respiratory chain 🥳
Using cryo-electron tomography🔬, we show how the respiratory complexes (and other complexes) are organized inside native mitochondria! 
https://t.co/cxHl1j20CJ

[Pagliarini Lab @Pagliarini_Lab]

Many thanks for all the very kind messages! My team and I are excited by all the ways this will empower our science, hopefully in collaboration with many of you.

[Andrew Smith @AJsmith231]

@nieminm @NatureComms Addendum: A special acknowledgmenet for the environment

[Andrew Smith @AJsmith231]

It looks like phosphorylation in mitochondria is a pretty important regulatory mechanism 👀

[Pagliarini Lab @Pagliarini_Lab]

I’m pleased to share our new work on the mitochondrial phosphatase PPTC7 led by @nieminm! Check out the important roles this protein plays in mitophagy and metabolic regulation.

#mitochondria
https://t.co/sepxHqsu0B

[Natalie Niemi @nieminm]

Very (very!!) happy to share our latest publication, "PPTC7 maintains mitochondrial protein content by suppressing receptor-mediated mitophagy", out today in @NatureComms. 1/n https://t.co/zq4JjjyiC4

[Pagliarini Lab @Pagliarini_Lab]

Happy to share our new mitochondrial proteome research review out today in @NatRevMCB led by @Zak_Bakes and @PatrickForny. The field has come a long way in the past 20 years, yet plenty of exciting new things to do!
https://t.co/9ovfanVCsm
https://t.co/sHj3bT2Z91
#Mitochondria

[Andrew Smith @AJsmith231]

@EdreesRashan Look no further
https://t.co/yAgrWLGmeS

[Pagliarini Lab @Pagliarini_Lab]

Our lab is now accepting applications for a new Instructor (faculty-level) mass spectrometrist position.
@mitojobs
https://t.co/ThzbHGwXIU

[Pagliarini Lab @Pagliarini_Lab]

I am happy to share (a bit late) our new review on coenzyme Q biology published recently in @TrendsBiochem . Congratulations to Dr. Rachel Guerra for leading the charge!
Coenzyme Q biochemistry and biosynthesis https://t.co/n2wdwIYWzk

[⭐ amy o'connor ⭐ @amyohconnor]

Good evening, gang!

I have once again compiled a thread of little bits from 2022 that I found funny, heartwarming, inspiring or just plain silly. It can be tough out there so sit back, relax and hopefully something in this thread might make you smile!

Let’s go! 💕