Alpay Burak Seven

Structural Biologist & Biochemist. Studying GPCRs. Loving CryoEM, NMR and all the fluorescent techniques.

[Cornelius Gati @CorneliusGati]

Excited to share our work on GPCR antagonism: Contrary to popular belief, inverse agonists can bind to and act via GPCR:G protein complexes. We show this at kappa-opioid receptors by cellular, radioligand binding and cryoEM studies. A 🧵: @USC @USCDornsife https://t.co/7EN1pVc3Fd

[Alpay Burak Seven @alburse]

@IsraelF96135088 Magnification calibration opportunity.

[Alpay Burak Seven @alburse]

Congrats to @GabriCorso and the team!

[Alpay Burak Seven @alburse]

Finally one out with "real" open source license. It is hard to understand Google's strategy for keeping AF3 under non-commercial license considering it took only a couple months to replicate AF2.

[Gabriele Corso @GabriCorso]

Thrilled to announce Boltz-1, the first open-source and commercially available model to achieve AlphaFold3-level accuracy on biomolecular structure prediction! An exciting collaboration with @jeremyWohlwend, @pas_saro and an amazing team at MIT and Genesis Therapeutics. A thread!

[Alpay Burak Seven @alburse]

@klausenhauser @schwabpa We read many of the yeast genes like some IDs.

[Alpay Burak Seven @alburse]

@liu_hanjin Great tool.

[Alpay Burak Seven @alburse]

BioNumPy!
https://t.co/h1fhcpf5WW

[Alpay Burak Seven @alburse]

@jjanetzko @IsraelF96135088 @IsraelF96135088, will you share your experience with preparing gold grids and apoFe? I believe Thermo is currently using their new VitroEase product.

[Alpay Burak Seven @alburse]

@jjanetzko @IsraelF96135088 They make their own apoFe and gold grids according to his post while back.

[Alpay Burak Seven @alburse]

@EAriasPalomo @CIB_CSIC @SMasiulis any luck on other proteins?

[Alpay Burak Seven @alburse]

@IsraelF96135088 @AmaiaGM17 That is an amazing image!

[Ernesto Arias-Palomo @EAriasPalomo]

The apoferritin test data collected using the TalosL120C + Ceta-F at the EM facility @CIB_CSIC with @SMasiulis finally reached ~4.5 - 5 Å! 😳

We are frankly surprised by the performance of the LaB6 filament with a Ceta-F camera, More details below 👇

[Alpay Burak Seven @alburse]

@IsraelF96135088 Looks thin.

[LolandLab @cloland_dk]

Here we go! Our structure of the human dopamine transporter in complex with cocaine has been published in Nature (https://t.co/A2yyoJwGsC). Commentary by @SitteHarald (https://t.co/PxuXLQ8qGS). Thanks to @ShahsavarAzadeh and Pape for contributing. @neuroscience_dk, @UCPH_health

[Alpay Burak Seven @alburse]

@meelonmuskusa @elonmusk, how about OS? If shipped with a new OS compatible with Linux, then I would buy one every 2 years for life.

[Israel Fernandez @IsraelF96135088]

👇 top!

[Alpay Burak Seven @alburse]

@IsraelF96135088 Do you see the circle at the same diameter if you do the gain at a different mag?

[Alpay Burak Seven @alburse]

@Q_M_Smith @IsraelF96135088 The holes are too small. You need very high magnification and a very well aligned scope to do a decent AFIS collection. I think they should make 0.6 or 1 micron holes.

[Alpay Burak Seven @alburse]

This is amazing!

[Hari Venugopal @HariVenugopal2]

Dear #cryoem comrades. Check out our preprint on how 100 keV detectors help breathe fresh life into ubiquitously available LaB6-based TEM workhorses. A collaboration with @GatanMicroscopy, @GeorgRamm, and @CryoEM_Monash. https://t.co/NOo13IZtqN

[Alpay Burak Seven @alburse]

@MFPronker Great work. Quick question, the protein sequence changed slightly from the full antibody sequence that was reported in the previous study. Was the change for protein behavior?