Alejandro Arce R.

Molecular microbiologist intrigued in AMR💊, metabolism⚙️ and gene regulation🧬 of pseudomonads. Senior researcher at Dept. of Microbiology, Univ. de Sevilla

[Alejandro Arce R. @aleyo_arce]

🚨 New paper out! Collaboration between @hausslerlab, @tuBraunschweig, and @PabloINik, @LabNikel
In this protocol, we describe a #CRISPR-Cas9 -Recombineering system for the genetic #engineering of #Pseudomonasaeruginosa lab strains and clinical/environmental isolates (🧵 1/7)

[Nature Protocols @NatureProtocols]

#NewNProt: New #CRISPRCas9 -assisted #recombineering tools allowing genetic editing of intractable
#Pseudomonas aeruginosa isolates https://t.co/b8MgvsHLHP @HausslerLab @Helmholtz_HZI @tuBraunschweig

[Ruggero La Rosa @rug_lrs]

Our group is looking for a PostDoc with a strong background in molecular microbiology, strain design and genetic engineering. Join us at @Rigshospitalet and @DTUtweet to work on bacterial infections and #AMR. Please RT and apply!
https://t.co/tInWJsFkRA

[Akos Nyerges @AkosNyerges]

Quadruplet codon decoding-based versatile genetic biocontainment system
https://t.co/QFtAIlreRL

[Patricia Bernal @pbernal209]

Fantástico Andony, has hecho un trabajo excelente de la mano de @aleyo_arce Mucha suerte en tu búsqueda de tesis doctoral y en nuestro lab siempre tendrás tu casa 👏🏼👏🏼👏🏼

[Pablo Iván Nikel @pabnik.bsky.social @PabloINik]

The #Microbes for #sustainability workshop #Baeza @AytoBaeza continues w/a great example of #bacterial #biocontrol by @pbernal209, complete w/a full mechanistic explanation of how Type VI #secretion systems work in #Pseudomonas @UniversidadAnd

[Patricia Bernal @pbernal209]

Absolutely honoured to host Microbiology legend Ken Timmis @KennethTimmis at @unisevilla this week! His talk on International Microbiology Literacy Initiative was truly inspiring. Grateful for invaluable stories, advice & experiences shared. It was very special to have him here.

[Pablo Iván Nikel @pabnik.bsky.social @PabloINik]

Off to a good start in 2024! Thrilled to share the pAblo·pCasso toolset for unconstrained cytidine/adenine-base editing in #bacteria led by @KaterinaKozaeva w/Zach Nielsen & @ManuelNietoDom1 @LabNikel @DTUBiosustain🦠@NAR_Open #SynBio #OpenAccess #CRISPR
https://t.co/xIBP1N87E8

[Microbial Biotechnology (MBT) @MicrobialBiote1]

Revolutionizing gene libraries in E. coli! Cerdan et al present a new approach for efficient integration of large gene libraries into the bacterial chromosome to address protein toxicity & instability issues. A new functional screening in biotech!  ➡️ https://t.co/y9qp2p3ptc

[Pablo Iván Nikel @pabnik.bsky.social @PabloINik]

#Environmental #Microbiology #workshop 2024 led by Juan Luis Ramos, @vdlorenzo_CNB & Puri López-García · Speakers include @pbernal209 @mSystemsJ @portokalica @KennethTimmis & others—applications open till Jan 15th @AMIposts #Baeza #Spain #biotechnology

[Alejandro Arce R. @aleyo_arce]

Former scientific colleagues, now friends for a life time 🫶🏻

[Víctor de Lorenzo @vdlorenzo_CNB]

Happy gathering of past, present & future scientific luminaries for joining the memorable celebration & wedding party of our dear friends and colleagues @Alberto_SPJ & @Mery_PipiS 👇

[Bruno Gonzalez Zorn @_bgzorn_]

Martes 21 de Noviembre a las 22:45 La Pandemia Silenciosa, India. En @laSextaTV

@CarlosMTBVAC @unicomplutense @SEMicrobiologia @SEIMC_ @microBIOblog @CSIC @AruUcm @PRANgob @jalisdelaserna @EnviadoTV @opsoms @vacunando @esmisionposible @animalshealthes @isanidad

[Víctor de Lorenzo @vdlorenzo_CNB]

Every Xmas season the @CNB_CSIC organizes a 1-day workshop for expat Spanish postdocs & young PIs abroad willing to share what they are doing and widen their network of local colleagues. Also, a chance to survey the domestic job market 😅. Please email organizers if interested 👇

[Alejandro Arce R. @aleyo_arce]

The availability of different AbR cassettes enables also genetic engineering of #clinicalisolates. We include also a whole section with guidelines and recommendations that facilitate the modification of these intractable strains (7/7)

[Alejandro Arce R. @aleyo_arce]

🚨 New paper out! Collaboration between @hausslerlab, @tuBraunschweig, and @PabloINik, @LabNikel
In this protocol, we describe a #CRISPR-Cas9 -Recombineering system for the genetic #engineering of #Pseudomonasaeruginosa lab strains and clinical/environmental isolates (🧵 1/7)

[Nature Protocols @NatureProtocols]

#NewNProt: New #CRISPRCas9 -assisted #recombineering tools allowing genetic editing of intractable
#Pseudomonas aeruginosa isolates https://t.co/b8MgvsHLHP @HausslerLab @Helmholtz_HZI @tuBraunschweig

[Alejandro Arce R. @aleyo_arce]

Both vector sets are available with several antibiotic resistance cassettes, which allows iterative engineering of different alleles without the need for plasmid curing. The complete toolkit can be purchased at @Addgene https://t.co/HPfsARnZ3K (6/7)

[Alejandro Arce R. @aleyo_arce]

Overall, the minimum time required to carry out a desired genomic modification is 5 working days, having already prepared all the materials required for the genetic engineering of a given lab, clinical or environmental strain (5/7)

[Alejandro Arce R. @aleyo_arce]

The mutagenesis oligonucleotide requires only 40-50 nt homology regions in order to introduce virtually any type of modification, including short and long deletions, short insertions, and SNPs with varying efficiencies (4/7)

[Alejandro Arce R. @aleyo_arce]

The second plasmid harnesses the inducible expression of a gene coding the powerful #Pputida Ssr recombinase described recently by @vdlorenzo_CNB. This protein promotes the recombination of a ssDNA mutagenesis oligonucleotide that introduces the desired mutation (3/7)

[Alejandro Arce R. @aleyo_arce]

Our method consists of a dual plasmid system. The first vector encodes an inducible cas9 and the constitutive expression of a target-specific sgRNA for the counterselection of WT alleles. The plasmid has already proven effective in the related bug #Pputida by @LabNikel (2/7)

[Nature Protocols @NatureProtocols]

#NewNProt: New #CRISPRCas9 -assisted #recombineering tools allowing genetic editing of intractable
#Pseudomonas aeruginosa isolates https://t.co/b8MgvsHLHP @HausslerLab @Helmholtz_HZI @tuBraunschweig

[Molecular Microbiology @CommichauM]

😳Genome-wide screening reveals metabolic regulation of stop-codon readthrough by cyclic AMP:

https://t.co/3kfSPq5YWZ