Annelie Marquardt

Scientist @CSIRO. Views are my own.

[Annelie Marquardt @amarqdt]

@wd_kate Thanks Kate!

[Annelie Marquardt @amarqdt]

When sugar builds up in the leaf - the final paper from my PhD was published this month. Truly grateful to everyone involved.
"Effect of sugar feedback regulation on major genes and proteins of photosynthesis in sugarcane leaves"
https://t.co/caw38mH44P

[Annelie Marquardt @amarqdt]

This is neat - ePaddocks is a new technology designed by @CSIRO to save time for farmers and others in the agricultural sector when using digital services for farm analytics and insights. #csiroag #csirospace
https://t.co/mgKpM6K7PR

[Maria de la Paz Vilas @mariapazvilas]

Our first #womeninsugar meeting! @amarqdt @annelrae @NeroliRoocke @sugarresearch

[Annelie Marquardt @amarqdt]

The setup 🌱

[Annelie Marquardt @amarqdt]

New glasshouse experiment currently underway - Jai compacting soil into PVC pots with a post-driver.
Looking for differences in root growth between #sugarcane varieties through tough soil & we’ve upped the soil strength this time #goodluckroots

[Annelie Marquardt @amarqdt]

A favourite photo :)

[sugarresearch @sugarresearch]

Check out the SRA Annual Report, hot off the press, including a stunning cover photo by @amarqdt https://t.co/GUPlkwIFqR

[Annelie Marquardt @amarqdt]

Definition of #potbound? Washing off the soil made us think about how this whole #rhizobox was pretty full with soil when we planted... and #roots don’t eat soil. Definitely some self-compacting going on. #sugarcane

[Dr Colleen MacMillan @ColleenMac3]

Having huge #fun at the @CSIRO Agriculture and Food Postdoc and PhD Forum 2019 in #Sydney over the last 3 days. What an intelligent and inquisitive bunch! @rick1mil @uday_kage @TansynNoble @WalshDominica @amarqdt @dphuber @MatthiasJost3 @ListenBach @mariapazvilas @JuliusKotir

[Annelie Marquardt @amarqdt]

@malinda2280 @sugarresearch We break the cores up in the field and put them into bags. But you can use plastic sleeves or cylinders to transport them out of the field. (If that is what you mean?).

[Annelie Marquardt @amarqdt]

4 stroke post driver = portable ‘core driver’

(Taking soil cores for Dr Johann Pierre’s CSIRO project on #sugarcane in-field root quantification, funded by @sugarresearch)

[Annelie Marquardt @amarqdt]

@bedeomara74 @sugarresearch We use a foot jack, you can see it here: https://t.co/Va5NJX8yup

[Annelie Marquardt @amarqdt]

@ChiNurse @RanaeDietzel @sugarresearch The post driver is about 15kg 💪

[Annelie Marquardt @amarqdt]

@Subsolum @sugarresearch This one is about 15kg - not exactly light, but not too bad: https://t.co/s3OwLHVy3S

[Annelie Marquardt @amarqdt]

@RanaeDietzel @wbrutter @sugarresearch We use what’s called a Sampler Foot Jack:

[Annelie Marquardt @amarqdt]

@wd_kate Thanks Kate!

[Annelie Marquardt @amarqdt]

#autofluorescence - so convenient.

Embedding and sectioning in agarose - also convenient.
#plantroots #sugarcaneroots #crosssection

[Annelie Marquardt @amarqdt]

@HausMJ @antonwasson Thanks! We used 3D printed molds from the paper: Atkinson, Jonathan A, and Darren M Wells. "An Updated Protocol for High Throughput Plant Tissue Sectioning." Frontiers in Plant Science 8 (2017): 1721. Then sectioned with vibratome. Let me know if you'd like any more information🙂

[Annelie Marquardt @amarqdt]

Please note I made an error: Dr Vijaya Singh is of the School of Agriculture and Food Science at the University of Queensland (UQ) - not QAAFI. Apologies!

[Annelie Marquardt @amarqdt]

Thursday’s #sugarcane root-reveal - #weirdlysatisfying.
We’ve been testing rhizobox methods (thanks to Dr Vijaya Singh of QAAFI/UQ).

[Annelie Marquardt @amarqdt]

@julyhappywood It is a way to wash roots grown in rhizoboxes to preserve their growth direction (and avoid tangling). I have been in contact with Dr Singh and will pass on links to papers where the method is described.