Alex Stein

[Alex Stein @astein7]

Very proud to introduce ScisorWiz - a tool for visualizing long-read, single-cell differential isoform expression! It's easy to use and customizable based on individual needs! Huge thank yous to @noush_joglekar, @cheesepuuff, and @hagentilgner! https://t.co/Mpejv7b68K

[Alex Stein @astein7]

@NICKMERCS Congratulations man! A beautiful healthy baby boy! We always knew it would be! A proud MFAM welcoming its newest member!

[Anoushka @noush_joglekar]

I'm so excited to FINALLY present the latest preprint from the @hagentilgner lab where we look at alternative splicing in the brain across "space and time" https://t.co/Mfx95oEE95

[Hagen Tilgner / @[email protected] @hagentilgner]

Our preprint on single-cell long-read isoforms across mouse brain regions, developmental time points as well as human hippocampi is here:
https://t.co/SnCXlXR3vp
Great team work with contributions by many but led by @noush_joglekar, who invested almost 3 years into this.

[Anoushka @noush_joglekar]

This tool is a godsend for anybody trying to visualize complex splicing patterns across multiple conditions. @astein7 did a fantastic job making it user friendly, intuitive, and with awesome interactive properties

[Hagen Tilgner / @[email protected] @hagentilgner]

Our long-read plot library is out. Led by @astein7 with
help by @noush_joglekar and @cheesepuuff:
https://t.co/fxjbTspif7
1 command for Figs 4e in https://t.co/6gHzbHRqgo 2F in https://t.co/J4e1ZxMXs8 5e in https://t.co/gJfCta7dgX 5F in https://t.co/GiYM7KtbCi @PacBio @nanopore

[Anoushka @noush_joglekar]

If you have seen any of our work, you know that we're extremely partial to pretty examples like this one ⬇️

Creating each one of these was always a frustrating task until @astein7 came along and made it a cakewalk. Try out his R package on your own data! https://t.co/vuZDqIMNjf

[Julien Jarroux @jjarroux]

ScisorWiz is out! New paper from the @hagentilgner lab with a tool to visualize single-cell isoform expression, by @astein7 with @noush_joglekar and @cheesepuuff
https://t.co/BuDLDs4d6a

[Hagen Tilgner / @[email protected] @hagentilgner]

We have been asked about our single-gene plots. The deal was 1) make huge coffee pot on Friday night; 2) while not good; do tweak bash code, then awk, then R for gene; done 3) plot ready on Saturday. Now @astein7 made it single-command: https://t.co/rCrEvjogJO

[Alex Stein @astein7]

ScisorWiz is a tool for visualizing Differential Isoform Expression using single-cell RNA-sequencing data. The paper is up on bioRxiv! Extremely proud of our work here. Thanks to @noush_joglekar, @hagentilgner, and Chi-Lam! https://t.co/5srOjItCB7

[Alex Stein @astein7]

Finally out: Our platform comparison for individual molecules:
https://t.co/5LxqhG1ctQ

[Alex Stein @astein7]

Big congratulations to the members of the Tilgner Lab at WCM and all collaborators on this @NatureBiotech paper on single-nucleus isoform RNA sequencing (SnISOr-Seq)! https://t.co/sLBmTIh60L

[Anoushka @noush_joglekar]

I am beyond thrilled to present our work on single-nuclei isoform sequencing (SnISOr-Seq), now out in Nature Biotech https://t.co/rl9UiyTYUv. Thread below ⬇️

[Hagen Tilgner / @[email protected] @hagentilgner]

Our new work on single-nuclei isoform sequencing is available on biorxiv: https://t.co/THTWHBAq7y Led by postdocs @si_hardwick & Wen Hu and PhDstudent @noush_joglekar but with contributions by many. Applied to human PFC and sc splicing, TSS, polyA sites and combination patterns.

[Anoushka @noush_joglekar]

Thought we'd start off '22 with a fresh serving of science on your timeline! 🧵

TL;DR we developed single-nuclei isoform sequencing in frozen human brain tissue and found some cool stuff: https://t.co/43KkStXF4Z

[Alex Stein @astein7]

I don't tweet often, but when I do, it is for a good reason! @noush_joglekar's hard-earned paper was published today and she was kind enough to include me in the list of authors and contributors! Congratulations to everyone involved!
https://t.co/6UP06SqzYT

[Hagen Tilgner / @[email protected] @hagentilgner]

Immensely proud of work led by first grad student (@noush_joglekar), but with crucial contributions by so many (please do read the author list): https://t.co/BjrG5IqC8Q
Single-cell isoform comparison of PFC and hippocampus and development of slide-isoform sequencing (Sl-ISO-Seq)

[Anoushka @noush_joglekar]

Our labor of love is finally out! https://t.co/gDffB5iwpr
A massive thank you to all our collaborators, without whom none of this would've been possible @ahmedElkoussy @bioinf_spbu @10xGenomics @SvenAT @bllapin @drisso1893 @erichjarvis @hagentilgner

[Hagen Tilgner / @[email protected] @hagentilgner]

Excited to share work led by grad student Anoushka & important contribs by many. @noush_joglekar https://t.co/CC5OhSqDWi

[Anoushka @noush_joglekar]

Excited to announce the newest preprint from the Tilgner lab - https://t.co/HEhePQugZb

We always knew that splicing is messy, especially at the single cell level, but we found that there's some method to the madness 1/n