Julien Jarroux

RNA enthusiast. PhD.
Before : Institut Curie.
Current : Tilgner lab at Weill Cornell Medicine

[Hagen Tilgner / @[email protected] @hagentilgner]

Our review of single-cell isoform work is out:
https://t.co/68awXMZMCy
By @noush_joglekar @CareenFoord @jjarroux and Shaun Pollard (not on twitter)
#LongRead #RNA @iRnaCosi #nanopore #pacbio #genomics #Transcripomics #RNAseq #Splicing

[Hagen Tilgner / @[email protected] @hagentilgner]

- finally cell-type specific exon inclusion in mouse hippocampus is largely conserved in human hippocampus, but human brain has turned extra exons that are constitutive in mouse into cell-type specific alternative exons in human.

[Hagen Tilgner / @[email protected] @hagentilgner]

- developmental changes (of matched cell types) stronger than brain-region differences (of matched cell types), and especially the P21-to-P28 transition stands out.

[Hagen Tilgner / @[email protected] @hagentilgner]

- for brain-region variability: some gene classes stand out, including neurotransmitter release and reuptake as well as synapse turnover
- for glia strong poly(A)-site regulation between brain regions,
- for neurons, also strong regulation at TSS

[Hagen Tilgner / @[email protected] @hagentilgner]

main results:
- for 3/4 of genes full-length isoform expression varies between brain regions (matched cell types), development (matched cell types) or between subtypes of a high-level cell type.
- on top: strong splicing variation between high-level cell types

[Hagen Tilgner / @[email protected] @hagentilgner]

Our preprint on single-cell long-read isoforms across mouse brain regions, developmental time points as well as human hippocampi is here:
https://t.co/SnCXlXR3vp
Great team work with contributions by many but led by @noush_joglekar, who invested almost 3 years into this.

[Julien Jarroux @jjarroux]

Check out the exciting new paper from the Tilgner Lab, looking at alternative splicing in the brain!

[Anoushka @noush_joglekar]

I'm so excited to FINALLY present the latest preprint from the @hagentilgner lab where we look at alternative splicing in the brain across "space and time" https://t.co/Mfx95oEE95

[Anoushka @noush_joglekar]

This was of course a culmination of many years of effort - many many thanks to all the brilliant friends, collaborators, and colleagues (on and off this platform) who made this happen: @rainryuhei_wen @MarkDiekhans @GSheynkman @ofedrigo @erichjarvis

[Anoushka @noush_joglekar]

I'll stop before I belabor this analogy, but go check out our preprint if you want to be blown away at the sheer amount of stuff that happens in the brain. I had a blast scrutinizing this amazing data from every angle 😍

[Anoushka @noush_joglekar]

And it seems that no matter where you are, teenagers (cell types in adolescent mice in this case) are wacky and rebellious, but they're ultimately just trying to find their identity

[Anoushka @noush_joglekar]

Splicing happens everything everywhere all at once. It's almost dizzying, the number of ways a gene can change its form depending on what environment it's being expressed in

[Anoushka @noush_joglekar]

We collected some very cool data to study isoforms across multiple scales in the brain: cell (sub)types, spatially distinct regions, and developmental timepoints. Very succinctly, what we found is that

[Anoushka @noush_joglekar]

I'm so excited to FINALLY present the latest preprint from the @hagentilgner lab where we look at alternative splicing in the brain across "space and time" https://t.co/Mfx95oEE95

[Hagen Tilgner / @[email protected] @hagentilgner]

After 12yrs in #longreads, the method of 2022 https://t.co/0FimVqi8J6 is a treat! Thanks @naturemethods for letting us write about it (link to follow). Led by @CareenFoord+@JustineKCH+@jjarroux. See also Andrey+Alla’s long-read software https://t.co/rMRZVblNQO

[WCM Neurogenetics @WCM_CNG]

Congratulations to Dr. Hagen Tilgner, Dr. Anoushka Joglekar, and Dr. Julien Jarroux, for their newly published paper, out today in Nature Biotechnology!
https://t.co/sbWrTyszyS

[Julien Jarroux @jjarroux]

Ever wandered how to get the best out of your long read RNA-seq?
Whether you use @nanopore or @PacBio, give IsoQuant a go for accurate isoform prediction! More in our newest paper from the @hagentilgner lab.
https://t.co/QmhzNviFXH

[Hagen Tilgner / @[email protected] @hagentilgner]

Out in @NatureBiotech: What isoforms are really in your @PacBio or @nanopore run? Andrey&Alla (with help by @noush_joglekar, AS, @jjarroux &AL) have precise answers:
https://t.co/2bmd5K0XkQ
#LongRead #RNA @iRnaCosi #nanopore #pacbio #genomics
#Transcripomics #RNAseq #Splicing

[Hagen Tilgner / @[email protected] @hagentilgner]

On Saturday, we welcomed girl scouts troop 2848 at @WCM_CNG and @WCM_BMRI for a session of Saturday Science. From strawberry DNA extraction to single-cell brain biology. So great to see these girls' enthusiasm and great work by @noush_joglekar @jjarroux and @rainryuhei_wen

[Julien Jarroux @jjarroux]

ScisorWiz is out! New paper from the @hagentilgner lab with a tool to visualize single-cell isoform expression, by @astein7 with @noush_joglekar and @cheesepuuff
https://t.co/BuDLDs4d6a

[Julien Jarroux @jjarroux]

At @WCM @WCM_BMRI, in @hagentilgner's lab with superstars @si_hardwick, @rainryuhei_wen and @noush_joglekar!

[Julien Jarroux @jjarroux]

New paper from our lab in @NatureBiotech!
If you're interested in RNA sequencing, isoforms, single-cell and single-nuclei transcriptomics, and gene expression in the brain, this is the one for you! https://t.co/DsuUGeVrx2