Dan Pollard Lab

Evolutionary genetics of gene expression at Western Washington University

[Dan Pollard Lab @dan_pollard_lab]

Thanks Joel and Heather. Looks like we might be one of the first to give it a go.

[Dan Pollard Lab @dan_pollard_lab]

Yeast RNA cross linking question. Does anyone know of a lab that has used psoralen TEG azide successfully in yeast? This is a the psoralen from the COMRADES method that allows for biotin to be added after RNA purification using click chemistry. Thanks! #yeast #rna

[Dan Pollard Lab @dan_pollard_lab]

Very excited to share our latest paper. Natural variation in codon bias and mRNA folding strength interact synergistically to modify protein expression in Saccharomyces cerevisiae https://t.co/V0PxPhqY2X

[Dan Pollard Lab @dan_pollard_lab]

Journal club paper today: Genome-wide base editor screen identifies regulators of protein abundance in yeast https://t.co/vzaEV6VTVA

[Dan Pollard Lab @dan_pollard_lab]

Journal club papers from a few weeks ago: Mechanisms of regulatory evolution in yeast https://t.co/uzv3wsWUqN Pleiotropic effects of trans-regulatory mutations on fitness and gene expression https://t.co/JCUCH9RpOu

[Dan Pollard Lab @dan_pollard_lab]

Congratulations to Pollard lab member @benjamin_haagen for receiving the 2023 WWU Biology Graduate Teaching Assistant Award!

[Dan Pollard Lab @dan_pollard_lab]

Thank you to the Gasch lab for hooking us up!

[Dan Pollard Lab @dan_pollard_lab]

Yeast labs: Anyone have a plasmid for HO expression and either KanMX or HygMX that you are willing to share? If neither of those, NatMX? Thanks!

[Dan Pollard Lab @dan_pollard_lab]

@NeedhiBhalla @BrianMDonovan @jes_cerdena OK, thanks!

[Dan Pollard Lab @dan_pollard_lab]

Am I correct that a college-level knowledge assessment on the biology of identity (race, sex, gender, and/or ability) does not exist? Instruments that even partially cover this topic would be very helpful. Thanks! @NeedhiBhalla @BrianMDonovan @jes_cerdena

[Dan Pollard Lab @dan_pollard_lab]

Dan's band Spouse recorded this sad holiday classic over the weekend and released it today. Enjoy. https://t.co/MhlEeRL2jG

[Dan Pollard Lab @dan_pollard_lab]

And thank you @ASCBiology for inviting us to put this event on!

[Dan Pollard Lab @dan_pollard_lab]

Big thanks to the many eager and engaged participants and my awesome co-facilitators (Tom, @samjlord, & @FritzLaylin) who helped make the statistics workshop at #CellBio2022 a success!

[Dan Pollard Lab @dan_pollard_lab]

Great to be at #ASCB2022 . Enjoyed learning about new proximity labeling methods from Alice Ting's keynote.

[Dan Pollard Lab @dan_pollard_lab]

Next journal club paper: Barcoded bulk QTL mapping reveals highly polygenic and epistatic architecture of complex traits in yeast https://t.co/LAn4T03rBA

[Dan Pollard Lab @dan_pollard_lab]

Come be my colleague at WWU! We have four biology-related tenure track positions open: (1) Molecular Cell Bio w/ Biomedical Focus, (2) Terrestrial Ecology, (3) Biochem, & (4) Chem/Biochem/Sci Ed. Review begins Sept 19th! https://t.co/UI4ZKAIdo2

[Dan Pollard Lab @dan_pollard_lab]

Preprint up of @AWienecke, Maggie Barry, my paper: Natural variation in codon bias and mRNA folding strength interact synergistically to modify protein expression in Saccharomyces cerevisiae https://t.co/c389JAZhPw

[Dan Pollard Lab @dan_pollard_lab]

Check out this EP recorded by Pollard lab member Sam Herr during his senior year: https://t.co/m6rGOv6BLx

[Dan Pollard Lab @dan_pollard_lab]

@NeedhiBhalla Very sorry to hear about the pushback but thank you so much for sharing this. We are working addressing race, sex/gender, and ability throughout our curriculum and being prepared for pushback directed at specific instructor identities sounds critical.

[Dan Pollard Lab @dan_pollard_lab]

Next journal club article: Genetic Analysis of Variation in Transcription Factor Binding in Yeast https://t.co/O7mSrB8qDQ