David Stokes

Structural biologist and Cryo-EM practitioner. Interests in membrane transport, cell biophysics and bicycles.

[David Stokes @dls4n]

Our lab is located at @nyugrossman, which is located in New York City. We have an energetic group and a vibrant biophysics community at the Med Center as well as active international collaborations @BjornPPedersen @orbeckst @khandeliagroup

[David Stokes @dls4n]

I'm looking for a postdoc to work on the ATP-dependent pump KdpFABC. We use biophysical tools like cryo-EM, transport, binding assays, smFRET to understand structure-function relationships and energy coupling. https://t.co/Mh19PKDTT7. 🙏spread the news or reach out with questions

[David Stokes @dls4n]

#PayItForwardLIVE

[David Stokes @dls4n]

@pollyp1 @RockefellerUniv @MBLScience David was a friend and a scientist you could trust. I will miss him greatly.

[David Stokes @dls4n]

@profLemieux @danengw Very well deserved and a long time coming. Congratulations Joanne!

[David Stokes @dls4n]

After a year and a half of planning and a little help from my friends, the cryo-EM facility at NYU is finally collecting its first data set for me tonight. The first of many more to come.

[David Stokes @dls4n]

Awesome display of cellular machines at work https://t.co/okmkDtcFij

[Janet Iwasa @janetiwasa]

Life cycle of #HIV now available to view & download at https://t.co/WnMFWOSkek!

[David Stokes @dls4n]

It was great to have the kids around, even attending some of the talks https://t.co/oNQc5VhGR5

[Joanne Lemieux @profLemieux]

Reflecting on the @CSMB_SCBM @MembraneMeeting held last week in Banff, @hyoung_howard and I were thrilled to bring our three kids along and see many other scientists doing the same. It may not be perfect (sleep'n with an 11yo starfish!) but many made it work. #worklifebalance

[Biophysical Society @BiophysicalSoc]

Are you a student interested in taking on a leadership role and helping to promote the field of biophysics at your school? Find out how to form a BPS Student Chapter on your campus and apply by May 15 for recognition in 2018: https://t.co/x7MdpNz1su

[David Stokes @dls4n]

Scientists accidentally create mutant enzyme that eats plastic bottles https://t.co/rj9qxM6wjQ

[David Stokes @dls4n]

sesame is great. image of sample matrix is very helpful.

[David Stokes @dls4n]

p2a3 reconstitution looks like aggregated/precipitated protein. definitely no vesicles. but I think you got the recipe wrong as we discussed

[David Stokes @dls4n]

DMPC liposomes prepared by biobead removal of C12E8 look good. Egg yolk are ok. Others not so good. Try again.

[David Stokes @dls4n]

DMPC liposomes prepared by reverse phase look excellent

[David Stokes @dls4n]

If the robot is not crashing the microscope, then why not let it go overnight. I don't think any harm comes of a vacuum crash anyway.

[David Stokes @dls4n]

Most vesicles look ok. EYPC/EYPA seems best, maybe neg charge causes increased adsorption to grid. What happened with DOPG?

[David Stokes @dls4n]

maybe better to let Changki do this. He should have conditions to evaluate the sample. Important to run standards!

[David Stokes @dls4n]

Will be important to use TLC to characterize this MHJF prep. I'm curious to see if detergent exchange was complete.

[David Stokes @dls4n]

Leginon modifications back to Anchi and Jim to incorporate into their distribution? Or do we have to remember this next upgrade?

[David Stokes @dls4n]

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