Justin Donnelly ⌬

Scientist-entrepreneur, drug hunter, rare disease champion, glyco geek 🍭. SF gay 🏳️‍🌈, )’(, skier ⛷️, traveler ✈️, 🐈-dad. 🇬🇧/🇪🇸/🇩🇪

[Justin Donnelly ⌬ @drjdon]

We found that 48/80, an MRGPRX2 agonist and commonly used mast cell activator, is toxic in HMC1.2 (human mast cells) and RBL-2H3 (rat basophils). Researchers should use caution and incorporate a viability test when performing assays in these cell lines. https://t.co/L6gfbpYmnI

[Aaron Ring @aaronmring]

@SynBio1 All of these companies are Just Another Wannabe Adimab. It doesn't matter that we can make antibodies a week or a month faster. We are in a massively target constrained environment not a binder constrained environment.

[Justin Donnelly ⌬ @drjdon]

@BecomingCritter Sunscreen

[alex rubinsteyn @iskander]

I think this was part of the implicit premise of the first incarnation of Hammer Lab at Mount Sinai, about a dozen of us with math/CS backgrounds ditched tech for biomedicine.

And we got humbled hard: most of what we did flopped & techies don't understand experimental design.

[alex rubinsteyn @iskander]

@paulg But it's a big tell that someone hasn't really ever grappled with the difficulties of actually making a confident discovery from biological messiness to think that the missing ingredient is people who took more signal processing classes or imbibed more essays on tech startups.

[Arcadia Pubs @ArcadiaPubs]

#PubDrop https://t.co/nBrTRQcIsL

[Justin Donnelly ⌬ @drjdon]

A spinoff of “The Americans” except it’s just the Deel spy at Rippling

[Justin Donnelly ⌬ @drjdon]

Have you used 48/80? How do you reliably measure mast cell activation? Let’s discuss! Comment on our paper or reply here to join the conversation. Please share this thread if you find this surprising or useful!

https://t.co/L6gfbpYmnI

[Justin Donnelly ⌬ @drjdon]

How did we discover that the widely used mast cell activator 48/80 actually kills mast cells rather than selectively activating them? Here's the surprising story 🧵 (1/12)

https://t.co/L6gfbpYmnI

[Justin Donnelly ⌬ @drjdon]

https://t.co/RYyDMnxobn

[Justin Donnelly ⌬ @drjdon]

Thread 🧵 to follow - stay tuned!

[Justin Donnelly ⌬ @drjdon]

We found that 48/80, an MRGPRX2 agonist and commonly used mast cell activator, is toxic in HMC1.2 (human mast cells) and RBL-2H3 (rat basophils). Researchers should use caution and incorporate a viability test when performing assays in these cell lines. https://t.co/L6gfbpYmnI