DRSC-BTRR & DRSC-TRiP at Harvard Med School

Account for the DRSC-BTRR (NIH P41 GM132087), current iteration of the DRSC & TRiP, a community-focused facility for fly and other insect genetic technology R&D

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Check out this video featuring @NIH_ORIP funded projects and highlighting opportunities for collab

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Big thanks to these related groups and funders & the whole #Drosophila community! @NIGMS @DrosDGRC @flystocks @FlyBaseDotOrg @alliancegenome @PerrimonLab

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Check out this video featuring @NIH_ORIP funded projects and highlighting opportunities for collab

[FlyBase @FlyBaseDotOrg]

#DIOPT 9.1 now also includes predictions for A. gambiae (African malaria mosquito) and E. coli. (Thanks to @rmwaterhouse for doing a release tweet for us!)
@DRSC_TRiP

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

New tech: Genome-wide CRISPRa screening in fly cells!

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Our newest online resource, PANGEA, and many others for #Drosophila, other genetic model organisms, mosquitos, and more can be accessed at our 'tools overview' page:

https://t.co/U6xxD5nHF7

[FlyBase @FlyBaseDotOrg]

New FlyBook chapter:
Visual processing in the fly, from photoreceptors to behavior https://t.co/PrwZC0pU5Q

[Drosophila Genomics Resource Center @DrosDGRC]

ModERN consortium project #Drosophila

[Troy Littleton @JTroyLittleton]

Drosophila community friends - please help spread the word for nominations for the Elkins Memorial Lecture at the 2023 Cold Spring Harbor Neurobiology of Drosophila Meeting. It’s a great opportunity to support and recognize our graduate student colleagues in the field!

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

If you're planning to make a KO cell line, a GLAD "Find group membership" search with your target gene can tell you if the gene is on this list--i.e., tell you if there's evidence that the KO you're trying to make is likely to result in non-viable cells

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

New at our GLAD online resource, list of genes that 'dropped out' in a genome-wide CRISPR screen in S2R+, suggesting they are essential for growth and/or viability in this cell line

GLAD:
https://t.co/e4IIBzmRq7

Data source:
https://t.co/jGvsywwOLZ

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Save the date, Boston fly folks -- the 2023 Boston Area Drosophila meeting will be June *14*!

https://t.co/E4eYghHP7h

[Fly-EDS @Fly_EDS]

Calling all European fly researchers! The 2023 European Drosophila Board elections are open! Closing date is May 19th. The candidate statements and voting link are on the EDS web site: https://t.co/MGMIycRTV9
Make your vote count! Results announced @EDRC2023

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

New tech: Genome-wide CRISPRa screening in fly cells!

[Perrimon Lab @PerrimonLab]

New paper: Xia, B., Viswanatha, R., Hu, Y., Mohr, S. E. and Perrimon, N. (2023) Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophilacells. Elife 12:e85542. doi: 10.7554/eLife.85542. PMID: 37078570

[FlyBase @FlyBaseDotOrg]

PANGEA allows single or multiple lists of genes to be computationally compared against various gene sets, like GO annotation and Reactome pathways. PANGEA includes many gene sets not available in other enrichment tools.

[FlyBase @FlyBaseDotOrg]

We've added a new graphical display for the Fly Cell Atlas single-cell RNA-Seq data, found in the “High-Throughput Expression Data” subsection of the Expression section of Gene Reports. This new graph shows how a gene is expressed across the major cell types of the adult fly.

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Excited to see use of our NanoTag technology reported in a preprint from the Gelfand lab:

Drosophila oocyte specification is maintained by the dynamic duo of microtubule polymerase Mini spindles/XMAP215 and dynein https://t.co/WRi5u75Mh7

[FlyBase @FlyBaseDotOrg]

Today's #FlyBaseTweetorial goes out to the user who wanted to know how to find a cDNA for a specific #transcript_isoform at #DROS23. We showed him the hard way to do it, and remembered the easy way a few minutes later. FlyBase user studying kayak, this is for you.
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[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Starting very soon (10 AM Central Time):

If you're registered for ADRC #Dros23 you can visit the app to join today's virtual CRISPR workshop with presentations on technologies and resources you won't want to miss!

[CSHLflyneurocourse @CSHLflycourse]

Start working on those abstracts y’all! #cshlfly23 #SaveTheDate

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Multiplexed Transgenic Selection and Counterselection Strategies to Expedite Genetic Manipulation Workflows Using Drosophila melanogaster https://t.co/AaGKTzHWN5

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Check out this trio of #Drosophila protocols just out from Venken et al.

Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster https://t.co/LnDcx4HXlh

[DRSC-BTRR & DRSC-TRiP at Harvard Med School @DRSC_TRiP]

Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster https://t.co/VIZESqT9Xz