Ellen Rim

Plant immune signaling. PhD in Wnt signaling. Unsolicited Wnt/crop engineering punditry my own

[Ellen Rim @ellen_rim]

Leaving Twitter. Follow me on Bluesky @ellenrim.bsky.social
Don't want to consume/generate more content in Elon-contaminated waters and glad to see a mass exodus of academics

[Ellen Rim @ellen_rim]

@Dani_M_Stevens @kseniakrasileva Aw thanks Dani :D

[Ellen Rim @ellen_rim]

Part of my postdoctoral work in the Ronald lab is on bioRxiv! We applied directed evolution to engineer a new-to-nature immune recognition range in a rice immune receptor

[bioRxiv Plant Bio @biorxiv_plants]

Directed evolution of a plant immune receptor for broad spectrum recognition of pathogen effectors https://t.co/GoSvBQpIEq #biorxiv_plants

[Ellen Rim @ellen_rim]

@CameronWBerry Aw thanks Cameron <3

[Ellen Rim @ellen_rim]

@JongminKmg Thanks Jongmin, hope youre doing well at Cornell!!

[Ellen Rim @ellen_rim]

We are testing the engineered receptors in plants now and hope to add plant data soon 🙂

[Ellen Rim @ellen_rim]

We evolved immune receptor binding to two evolutionarily divergent groups of ligands from a fungal pathogen. Such a receptor would protect from a broader spectrum of pathogen strains and reduce the likelihood that the engineered receptor will be "overcome" by pathogen evolution

[Ellen Rim @ellen_rim]

This shows how directed evolution can explore large synthetic sequence spaces to develop immune recognition of divergent pathogen ligands, a function that seemed initially far-fetched

[Ellen Rim @ellen_rim]

-TnpB (400aa small Cas12-like nuclease) can be used to genome edit plants
-Viral delivery of TnpB+guide using tobacco rattle virus led to somatic edits in arabidopsis
-Efficiency varied by plant and gRNA but up to 80% and there was some germline transmission

[bioRxiv Plant Bio @biorxiv_plants]

Viral delivery of an RNA-guided genome editor for transgene-free plant germline editing https://t.co/B0YCvD22Uw #biorxiv_plants

[Lay Teng Ang @anglayteng]

The Ang lab at Stanford University is looking for students, research professionals, and postdoctoral fellows excited about stem cell biology and regenerative medicine. For more information, please email [email protected]

[Ellen Rim @ellen_rim]

@GittaCoaker @pcronald Thanks Gitta! I hope your recovery is going well

[Ellen Rim @ellen_rim]

Got to present my postdoc work directed evolution of a rice immune receptor in @pcronald lab at #PlantBio2024 in Hawaii. The talk was well received by the 6mo old

[Ellen Rim @ellen_rim]

Maternity leave thoughts: How do mammary glands expand during pregnancy and lactation? How do they develop? Led me to @wntlab papers while pumping

[Ellen Rim @ellen_rim]

via ⁦@nytimes⁩ the most inspiring profile I’ve read in a while
Also “The bench is there, the science is good,” she shrugged in a recent interview. “Who cares?” https://t.co/9PcRZurbvd

[Ellen Rim @ellen_rim]

@Dani_M_Stevens Amazing work, congrats Dani!!

[Ellen Rim @ellen_rim]

@wntlab Beautiful! Congratulations to Yorick!!

[@pcronald @pcronald]

Happy that our paper with Guotian Li is now published. This study highlights the power of genome-editing technologies in uncovering specific genetic variants associated with a desirable agronomic trait: disease resistance https://t.co/fW4TtmWzzC @JBEI @igisci @ucdavis @Nature

[Gitta Coaker @GittaCoaker]

Preprint has been out, but glad to have this paper published. Here we investigated immune responses across 86 citrus and citrus relatives (Rutaceae). 🧵

[Ellen Rim @ellen_rim]

Using this assay, two new wheat stem rust AVRs were identified from a library of 700 candidates 👏

[Ellen Rim @ellen_rim]

Protoplast (plant cell culture) and FACS-based assay to identify which AVR (pathogen-derived ligand) a given immune receptor binds

Pooled effector library screening in protoplasts rapidly identifies novel Avr genes https://t.co/2SezWuT5Pi