(1/10) We combined #AlphaFold and #CryoEM to derive atomic models of MegaDalton protein community members from cell extracts! The efforts from the #KastritisLab together with @ktototak were extraordinary to reveal their architectures - see thread below:
https://t.co/be3ypedACz
We're hiring postdocs. Think deep homology meets proteomics. Discover ancient biochemical systems, invent new tech, or study the structure/function/evolution of proteins in embryonic development. Please RT!
Email CV, interests, & references' contact info to [email protected]
We visualized a transient catalytic chamber on the giant pyruvate dehydrogenase complex from native cell extracts!The motivation was extraordinary to study this target with an array of methods! @imjohnskal@ctueting @hamdiphilum @FJ_OReilly@RappsilberLab https://t.co/mfgZf8Vwwg
Link to the paper - https://t.co/tA47pJbIR4
A quick thanks to my advisors @davidwtaylorjr, @edward_marcotte and colleagues who helped on this project (Yi Zhou, Anna Mallam, Andrew Horton). Also thanks to @3blue1brown for the open source animation software.
Earlier this year we shared an approach to help sort single particle cryo-EM data when there are multiple distinct structures present (i.e. non-purified samples). Here’s a brief animation showing the process.