Fraser Johnson

🇨🇦 Postdoc at Netherlands Cancer Institute 🇳🇱 Lindeboom Lab 🧬 Stepping into the TCR-pMHC interaction space—one experiment at a time 🧪

[Samuel Hume @DrSamuelBHume]

Novartis' new malaria treatment cured 97.4% of patients – more than the current best treatment

It kills resistant parasites, too, and probably blocks transmission better than current drugs

Approval is expected next year!

[Supratim Datta @taurine__]

The seminal paper of Shimon Sakaguchi published in 1995 @J_Immunol where he discovered CD4+ CD25+ cells that actively suppress autoimmunity. It was the first time T regulatory cells appeared in the literature. Not all Nobel Prize winning work need to be in glamorous journals.

[Klebanoff_Lab @KlebanoffLab]

🤯Three (!!!) new papers today in @ScienceMagazine on the application of generative AI for the de novo design of peptide/HLA binding molecules! Completely unique 3D structure and binding mode compared with natural TCRs and TCR mimics! Links to papers 👇

[Ly Vu @Vuphuongly]

Excited to share our first study using nanopore direct RNA seq to comprehensively characterize RNA features. We believe that our deep and high coverage datasets should be a nice resource for the field! https://t.co/xlMO7DyZtc

[Sebastian Gregoricchio, Ph.D. @s_gregoricchio]

🚨Our latest paper out on @GenomeBiology!
We observed a high epigenetic reprogramming in endometrial tumor development, with highly interesting genome/epigenome interplay in endometrial tumorigenesis and disease progression. Many thanks to all coauthors!!
https://t.co/5naKYmt3iX

[Sebastian Gregoricchio, Ph.D. @s_gregoricchio]

📢 Do you want to know more about ATAC-seq and it's applications? In this case join the @oncodeinstitute
workshop that will take place the 19th of September at the @NKI_nl!!
Places are limited to 35!! Subscribe at the following link:
https://t.co/ex2YmfaI6e

[KMcNagny-Lab, Biomedical Miracles at work @KMcNagnylab]

Canada's first purpose built Biomedical Engineering building. So great to see a timely win for those who believe in the benefits of science and translation and their ability to improving lives.

[Repare Therapeutics @RepareRx]

We are pleased to share that our research exploring lunresertib+camonsertib in CCNE1 amplified #ovarian and #endometrial orthotopic PDX models has been published in @NatureMedicine. Read the study here: https://t.co/mZ3miztIsj #precisiononcology

[Fraser Johnson @FJ_Scientist]

@bencoates1 Ordered! 👌

[Feng Zhang @zhangf]

We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGRs consist of a peculiar repeat region which is transcribed into RNA and processed into multiple guide RNAs to direct TIGR-associated proteins to their DNA targets. https://t.co/ketXqFVQqw

[Tanentzapf Lab @TanentzapfLab]

Reading a nice polished CV is useful & everything but the most important part of my scientific training, the 18 months I spent on a project that failed, which taught me resilience, problem solving, humility, & other such wisdom is nowhere to be found on my CV. So CVs have limits

[Adam Grant @AdamMGrant]

How to be a better critical thinker:

1. Be 10% more skeptical of people you agree with—and 10% more charitable to people you disagree with

2. Look for flaws in ideas you like—and strengths in arguments you dislike

3. Learn from sources that engage with competing ideas

[Fraser Johnson @FJ_Scientist]

@ATinyGreenCell @pochekailov DHL will make more profit than Sergi 😭

[Fraser Johnson @FJ_Scientist]

@ATinyGreenCell @worksleeve Exactly! 🔥👌

[Fraser Johnson @FJ_Scientist]

@ATinyGreenCell @worksleeve You have good molecular bio protocols, not very amateurish 😂

[Fraser Johnson @FJ_Scientist]

@ATinyGreenCell @worksleeve I had no idea! 🔥

[Fraser Johnson @FJ_Scientist]

@ATinyGreenCell 😭

[Fraser Johnson @FJ_Scientist]

@SjoerdSlagman @ATinyGreenCell @WUR I second this!

[Fraser Johnson @FJ_Scientist]

@ATinyGreenCell I prepared and vacuum filtered 500 mL of TSS buffer, aliquotted and froze it down. I'll add 3 uL bacteria to 3 uL cold DNA, raise the volume with 30 uL cold TSS buffer and proceed as normal. Substituting TSS for LB in that step failed to produce colonies so I'm sticking to TSS.

[Fraser Johnson @FJ_Scientist]

@ATinyGreenCell My variation was to grow up 400 mL intraday (aiming to hit 0.35-0.4 OD600 but not critical if overshot), resuspend in 40 mL TSS, aliquot into 80 tubes of 500 uL. I'm finding the that 3-5uL bacteria consistently give me colonies and they're pretty resistant to freeze thaws so far.