Florian Ströhl

*This Twitter account is not monitored any longer. Please join me on LinkedIn for continued research updates.* --- Florian, PI in Advanced Imaging

[Florian Ströhl @florianstroehl]

@aandr314 @AndrewGYork showed it to me a few years ago and we've used it since to align our OPM. Not sure if that's the original source though.
It is possible without, but once you've experienced the comfort of it you wouldn't want to do complex alignments with just hands and posts.

[James Manton @JamesDManton]

This is _clearly_ one of the best ideas in imaging of the decade.

I strongly encourage people to play around with this if they have a few spare hours — watching a *dye* clear a tissue in front of your eyes for the first time is an amazing experience.

[James Manton @JamesDManton]

We present:

'Multispectral live-cell imaging with uncompromised spatiotemporal resolution'.

https://t.co/SXCLXYJ74z

1/16

[Florian Ströhl @florianstroehl]

Recording many image planes simultaneously is a great time saver for 3D imaging. A wonderful tool for such multifocus microscopy is @FourierPower's Multifocus Grating: Here is a short video on how they work and how to make them (without a cleanroom). https://t.co/Jq6zhvqYFB

[Florian Ströhl @florianstroehl]

You can read the full paper (open access) at https://t.co/dqRMvFCYPQ. Finally, thanks a lot to Jon-Richard Sommernes and @amsikking - it was a pleasure working with you on this project! 4/n

[Florian Ströhl @florianstroehl]

Snouty-style OPMs offer volumetric imaging with high spatial- and temporal resolution, but the setups can suffer from polarization-dependent light reflections which reduce the effective aperture and transmission in the remote image space. 1/n

[Florian Ströhl @florianstroehl]

If you want to figure out the theoretical limits of your own LSM system, we also provide a software tool that can simulate any light-sheet microscope. The tool has a GUI and is straightforward to use. 3/n https://t.co/S3OoLlIvtc

[Florian Ströhl @florianstroehl]

@AmirSTORMic @jsdaniel02 @xyzDrch @carlasssmith It's a single exposure in SOLIS: when you put a multifocus element in the detection path, then the entire lightsheet plane is mapped onto the rolling shutter line on the camera. Scanning the sheet then maps the 3D volume onto the camera plane with rolling shutter camera exposure

[Florian Ströhl @florianstroehl]

What a start to 2024. Nobel Prize winner @Eric_Betzig and his team not only built a SOLIS system. No, they built 4!
https://t.co/E1kjRFY8Dl

[Florian Ströhl @florianstroehl]

@jsdaniel02 @xyzDrch @carlasssmith You can probably add Hari Shroff's multiview confocal microscope to the list of oblique linescan microscopes with rolling shutter detection. SOLIS is a more than that though: it also uses multiplane detection to image the full lightsheet illumination and not just the line's focus

[andrey a @aandr314]

How to draw optical layout / schematics?
First, start with vector graphics tool. Inkscape is free and good.
Secondly, there are libraries of nicely drawn basic optical elements such as https://t.co/tqqeE0Yous @gwoptics & Alexander Franzen

1/N

[Florian Ströhl @florianstroehl]

@xyzDrch @EikonTX Very interesting paper and cool applications! It would be great to get in contact.

[Robert Haase @haesleinhuepf]

🤫 #chatGPT integration will be coming to @FijiSc's Script Editor #SneakPreview 🥳
Thanks for the support @ctrueden ! 🙌
https://t.co/iBGOWM5F7n

[James Manton @JamesDManton]

The slight disappointment with being scooped is more than compensated by the very nice results and demonstrations of reduced photobleaching/toxicity with NIR co-illumination in this paper by Ludvikova et al: https://t.co/pR7ugwK12k

[Florian Ströhl @florianstroehl]

When you show up to #FOM2023 with the whole squad :D
Fantastic presence and presentations by the #UiT nanoscopy group at this year's conference. And thanks to Olli for the video ..

[Florian Ströhl @florianstroehl]

Here's how we did it: https://t.co/hTv9gU0bW4

.. of course with complementary YouTube video: https://t.co/gVh6AqyLr2

[Florian Ströhl @florianstroehl]

https://t.co/1Z3jnNsYvZ

Fantastic work by Hari Shroff's group on 4-beam SIM.

[James Manton @JamesDManton]

An impressive experimental demonstration of 4-beam 3D-SIM for enhanced axial resolution, by Hari Shroff's lab: https://t.co/xEx5dTvyRc