GINCPM Protein Profiling (proteomics)

The de Botton Institute for Protein Profiling provides cutting-edge, comprehensive, and quantitative analysis of proteins in biological samples using mass spec.

[Yishai Levin @YishaiLevin]

Proteomics people, I need help troubleshooting a set of single cell runs. This is a SCOPEMS experiment, FACS sorting into 384 plate, digestion in the plate, TMT10, carrier of 100cells, controls and 6 single cells per TMT batch. I ran them on the Exploris 480+FAIMS (-45,-65)

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

We started another advanced course in proteomics. One week, 11 students, 3 tutors and lots of spectra.

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

Hands on training. #TIMSTOFPRO

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

Coming soon: mass spectrometry based, single cell proteomics

[Yishai Levin @YishaiLevin]

Automating SCOPEMS2 on the #Bravo for #singlecellproteomics. Mock run.

[Eric Topol @EricTopol]

Adding to the body of data for booster shot effectiveness, a new @TheLancet report of over 728,000 people w/ 3 shots vs >728,000 matched controls (2 shots):
93% reduction of Covid hospitalizations
https://t.co/cVpANpnkHs

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

Congratulations on a fantastic project! Its always a pleasure working with you!

[Straussman_Lab @StraussmanR]

(4/4) Many thanks to our collaborators: Hadas Reuveni, @baruchbarzel, @TPilpel, Amos Tanay, Yishai Levin, @LabGeiger, Jair Bar, @KaminskiSivan, Salomon Stemmer, @ihaviv, Shlomit Gilad, Amir Prior, @Zohar__Meir and all lab members https://t.co/dPzh8vBd7W

[Straussman_Lab @StraussmanR]

(4/4) Many thanks to our collaborators: Hadas Reuveni, @baruchbarzel, @TPilpel, Amos Tanay, Yishai Levin, @LabGeiger, Jair Bar, @KaminskiSivan, Salomon Stemmer, @ihaviv, Shlomit Gilad, Amir Prior, @Zohar__Meir and all lab members https://t.co/dPzh8vBd7W

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

@MattWFoster 😆

[David Morgenstern @DavidMorgenst17]

Hey #ASMS2021, come visit me at poster 255 today, and see how a small tweak makes a big difference in glyco enrichment! #glycotime

[Aaron Javitt @AaronJavitt]

Excited to share the next @YMerbl project where we used #CellularDumpsterDiving to find a new proteasome regulator of immunotherapy responses in lung cancer. Check out the @biorxivpreprint https://t.co/yJPuuchaDS and short thread 👇 (1/5)

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

TIMS-Tof Pro unboxed today. Super excited 🤩

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

Just completed protein extraction and total conc. measurement from 396 human brain samples. Going for glycoproteomic profiling in collaboration with Dr. Michal Beeri.

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

We are happy to share our joint paper with Dr. Shilo Rosenwasser, HUJI. Together, we developed a proteomics approach for global quantification of the thiol-proteome. https://t.co/AFaUnvvxzA

[Yishai Levin @YishaiLevin]

Proteomics folks out there, I have a question about correction for multiple hypothesis testing (FDR). We find a lot of experiments when Q vales are very high with p values very low. We validate the results and find they are indeed significant. What would you do in such cases?

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

TIMS Tof Pro arrived yesterday 🤟

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

We completed yet another basic course in mass spectrometry based proteomics. It��s so rewarding to see people who know very little about about this technology, complete the course with the ability to explain, in a presentation, the process of identifying and quantifying proteins.

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

https://t.co/CMeRcR0ibY thanks @Smith_Chem_Wisc your software plays a central role in this.

[GINCPM Protein Profiling (proteomics) @GINCPM_PP]

Our SPEAR method is online, waiting on pear reviews. A new redox-proteomics approach based on differential labeling of oxidized and reduced cysteines with light and heavy isotopic forms of commercially available isotopically-labeled N-ethylmaleimide (NEM). No need for enrichment

[Yishai Levin @YishaiLevin]

Occasionally we get samples with polymers. It turns out with FAIMS, we can gas-phase separate/filter them out. Pretty cool!! (lower is -45, upper is -65)