Héloïse Verdier

PostDoc @LEHNA_lab | Using #eDNA 🧬 for biomonitoring of freshwater ecosystems 🏞️

[Héloïse Verdier @heloise_verdier]

Hi #eDNA community! I'm looking for review articles or guidelines on the filtration and normalization of #eDNA metabarcoding data. Thanks for your help!

[Héloïse Verdier @heloise_verdier]

@Silverthorn_T Félicitations Teresa !!!! 🎉🎉

[Héloïse Verdier @heloise_verdier]

@AlejandroT_G Thank you @AlejandroT_G for these enlightening answers!

[Héloïse Verdier @heloise_verdier]

Since I started working on #eDNA (4 years ago), it is still very difficult to find guidelines on qPCR data processing and analysis (especially for microcosm experiments). Scripts and data are rarely available... This would be a great contribution to the eDNA field!

[Héloïse Verdier @heloise_verdier]

@Morgan_R_Ellis @KayCritchell Yes certainly, however I think that building common guidelines for eDNA analysis (whether by metabarcoding or qPCR) would really help move the issue forward and have more confidence in the results obtained with eDNA.

[Héloïse Verdier @heloise_verdier]

@AlejandroT_G And what should we do with these triplicates, average the three values (even if there is a zero) or remove the zeros and then average? I have seen many different things in the literature..

[Héloïse Verdier @heloise_verdier]

@AlejandroT_G In fact, I am looking for a "standardised" approach to clean and process raw qPCR data. For example: how to sort values for which we have a high Cq (the threshold of the Miqe guidelines may not be adapted to eDNA), what to do when the qPCR triplicates include one or more zeros?

[Héloïse Verdier @heloise_verdier]

@UniKentSEaK Wow this is really great! With all the eDNA articles coming out, I missed it. Thank you so much for sharing!

[Héloïse Verdier @heloise_verdier]

@OllyBerry4 @SeDNASociety Thanks for sharing!

[Héloïse Verdier @heloise_verdier]

Hi eDNA folks!! I missed the #eDNA2023... 😢 what are the next #eDNA conferences not to be missed this year? 🧬🧬

[Héloïse Verdier @heloise_verdier]

I wanted to say that I missed @AusNZeDNA!

[Héloïse Verdier @heloise_verdier]

@mohammadwazne @LEHNA_lab @lsimon_iso @stefankrauseh2o Félicitations Moha !!!

[Juliette Tariel-Adam @JTarielAdam]

Dear scientists🙂I am looking for 💰grants funding research experiments💰Do you have any ideas? Restrictions: I'm French, postdoc at an Australian university, studying tool use in coral reef fish (animal behaviour/cognition, fish/marine biology, not conservation-based research)

[Taylor Wilcox @theednaguy]

I'm really excited to share this new tool for #eDNA qPCR assay design! eDNAssay is a machine learning model that predicts assay specificity. It's based on extensive empirical data and performance is excellent. https://t.co/JaKSiKMZyt

[Pritam Banerjee @edna_pritam]

Finally, our idea came to print. @alpineedna @Kat_A_Stewart @drbarnes @ingridbunholi @CMAntognazza @Doi_Lab @heloise_verdier Here we present eDNA on Plant animal interactions (PAI). https://t.co/F2lO5GSSsT. Will appreciate to know your comments.
#eDNA #PAI #speciesinteraction

[Héloïse Verdier @heloise_verdier]

Happy to share the first paper of my thesis published in @molecology on the development of a 3D-printed #eDNA sampling tool!
https://t.co/adxnVMWfin
Many thanks to @Tristan_Lefebur @Marquette_chris & all co-authors
@LEHNA_lab @3dFAB_platform @EcoFlowS @UmrCarrtel #MATEIS

[Francois Keck @FrancoisKeck]

Very happy to see our meta-analysis comparing metabarcoding to traditional methods for biological community assessment accepted in @molecology
https://t.co/4lkhdPwfC9
With @altermatt_lab @rosiecblackman @CoutonMarjorie @jea9brant @DoKirschner @hengzhangzh

[The Asellus Consortium @TheAsellus]

#TristanLefebure highlighting the value of genomic comparative analyses using #asellus | #asellussymposium

[Héloïse Verdier @heloise_verdier]

@messamat Hang in there Mathis!! 💪