Hiroshi Kanno

[Hiroshi Kanno @hk_666Sy]

@farmistead @SerendipityWrk Thank you Fern. I'm looking forward to seeing your work!

[Hiroshi Kanno @hk_666Sy]

My #SerendipityPoster
Hiroshi Kanno, The University of Tokyo.
"High-speed single-pixel fluorescence imaging by frequency-division multiplexing"
I'm working on developing high-speed fluorescence imaging techniques. #SW_Fluorescence #SW_FlowCytometry @SerendipityWrk

[Hiroshi Kanno @hk_666Sy]

@PhillipCMcCann @SerendipityWrk Also, the imaging architecture of FDM (frequency-division multiplexed) microscopy utilizes intensity modulation of illumination just for speeding image acquisition up. But, this intensity modulation can be "accidentally" used for FLIM, too. Why can I stop applying FDM to FLIM?

[Hiroshi Kanno @hk_666Sy]

@PhillipCMcCann @SerendipityWrk Thank you, Phil. Fluorescence lifetime imaging microscopy (FLIM) is attractive because it enables quantitative imaging without being affected by some factors (e.g. fluorophore concentration). There are a variety of applications including pH, temperature, and oxygen imaging.↓

[Hiroshi Kanno @hk_666Sy]

@JGaladePablo @SerendipityWrk Thank you Julia. There are sub-millisecond biological events we want to observe, including calcium signaling and neural activity. Also, in order to observe such fast dynamics in 3D, high imaging speed becomes much more critical (e.g. neuronal activity of a whole C-elegans)