Phillip Charles McCann

2nd year masters student at the University of Tokyo
B.S. in OSE at the University of Arizona

[Phillip Charles McCann @PhillipCMcCann]

@YutaNakagawa5 @SerendipityWrk Good question. We have been interested in using FLETCHERS to identify fluorescent proteins due to its sensitivity (due to incorporated Raman), and its ability to break the color barrier of traditional fluorescence microscopy.

[Phillip Charles McCann @PhillipCMcCann]

Broadband low-frequency spectroscopy via time-domain fluorescence-encoding

The University of Tokyo

Phillip McCann

@Serendipitywrk
#SerendipityWorksop2020
#SerendipityPoster
#SW_Fluorescence
#SW_RamanSpec

[Phillip Charles McCann @PhillipCMcCann]

@YutaNakagawa5 @SerendipityWrk Good question. The easiest way would be to just change the laser from Ti:Sapphire (750-850 nm) to something that emits in the visible range. Of course, we would need to replace the other filters in the system as seen in the first figure. But that should be all that is required!

[Phillip Charles McCann @PhillipCMcCann]

@hk_666Sy @SerendipityWrk No reason, I now understand the purpose of the FLIM and what you are trying to look at. I think it is a good idea

[Phillip Charles McCann @PhillipCMcCann]

@mhloo93 need some serendipity huh???

[Phillip Charles McCann @PhillipCMcCann]

@mhloo93 Nice, how long is it expected to take. That does not sound too easy to me although I have far less know-how than you on the matter

[ぬ @nukkie66]

Welcome everyone to the Serendipity workshop 2020. I am one of the two chairs for this session. The poster A session is now open. Let's get started your posters presentation! #Serendipityworkshop2020 @Serendipitywrk #SerendipityPoster

[Mun Hong Loo @mhloo93]

#SerendipityPoster #SW_Microfluidics #SerendipityWrk

[Phillip Charles McCann @PhillipCMcCann]

@HenryMicro36 @SerendipityWrk Ahh, that make sense. Very important findings using just a FV3000 confocal! People recently (even in our lab) have been focusing more on yeast so this kind of discovery is impactful to our work!

[Phillip Charles McCann @PhillipCMcCann]

@GuolinYun @SerendipityWrk Haha, I am a huge fan of projects like these. You have to merge a little bit of fun into the science every once in awhile to get peoples attention!

What does that say "WOW" or "VOW"? and if "VOW" what does that stand for?

[Guolin Yun @GuolinYun]

This is my Serendipity workshop poster!
'Liquid Metal-filled Magnetorheological Elastomer (LMMRE) with Positive Piezoconductivity'
@Serendipitywrk #Serendipityworkshop2020 #SerendipityPoster #microfluidics

[Henry 6666 @HenryMicro36]

Scanning massive yeast-containing bioreactors, we learnt how "Picolitre scale cultivation of single yeast cells correlates with lab-scale culture".

Macquarie University

@Serendipitywrk
#SerendipityPoster
#SW_Microfluidics
#SW_SingleCell
#SW_CellBiology

[Ryo Nishiyama @Nishiyama323]

My #SerendipityPoster about "Super-multiplex Raman flow Cytometry! We realized super-multiplex flow cytometry with the use of Raman tag.
@SerendipityWrk
#SW_FlowCytometry #SW_RamanSpec #SW_SingleCell #UniversityOfTokyo #SerendipityWorkshop2020

[jeff @JeffHarmon05]

#SerendipityPoster
Jeffrey Harmon at the University of Tokyo showing work in high-throughput microalgae classification via FDM microscopy combined with machine learning.
•#SW_FlowCytometry •#SW_Fluorescence •#SW_SingleCell

[Phillip Charles McCann @PhillipCMcCann]

@RKinegawa @SerendipityWrk I think it worked well in this case

[Phillip Charles McCann @PhillipCMcCann]

@Nishiyama323 @SerendipityWrk Nishiyama, how many tags have you made up to now? I thought it was more than 15?

Also, how long does it take to make a single tag?

[Phillip Charles McCann @PhillipCMcCann]

@JGaladePablo @SerendipityWrk We are still looking into that. As of now, we can resolve a Raman spectrum from samples as dilute as 100 uM (far better than other CARS processes) however, we have reason to believe that single-molecule detection might also be possible and is something we are trying to reach!

[Phillip Charles McCann @PhillipCMcCann]

@KangHee_snow @SerendipityWrk Thank you for the question! The best benefit of the visible range is that there are many more dyes to use compared to the NIR range. This would allow for more dye colors and a broader application with this method