Really nice work showcasing how tuning chemical reactivity can improve spatial resolution for proximity labeling. Super cool.
Directed Evolution of APOX for Proximity Labeling Using Phenols with High Redox Potentials | ChemRxiv - https://t.co/jsf62mECvx
We're entering into a golden age in proximity labeling, and photocatalysis is a major new driver for uncovering biological mechanisms. Check out our review (@BenitoBuksh@HuthSean@DanielleMorganC ) of the latest developments out now in @CellChemBiol!
New year, new proximity labeling protocol! We can use red light to access trifluoromethyl carbenes from diazos for proximity labeling. Congrats to @crabaneros and @stavkariofillis for a great paper in collaboration with Merck!
https://t.co/KCjAul1cCi
In a major milestone for science, for medicine, and for patients, the world’s first CRISPR drug has been approved in the UK.
“A new treatment for sickle-cell disease and transfusion-dependent β-thalassemia has been authorised by the Medicines and Healthcare products Regulatory Agency (MHRA) for patients aged 12 and over after a rigorous assessment of its safety, quality and effectiveness.”
Congratulations to the many scientists, doctors, patients, and industry leaders who made this development possible.🍻
https://t.co/pUL956YsG3
The Geri Lab is recruiting postdoctoral scholars to develop photocatalytic proximity labeling and proteomics technologies. Chemical biologists and chemists who want to learn chemical biology are welcome!
https://t.co/smnCJo39gj
The view from our recently expanded NYC lab space:
Excited to share our latest work on small molecule binding site ID. We demonstrate this application across 7 different protein-ligand pairs and in live cells. Really fun team effort with @HuthSean @CiaranSeath @Merck and the rest of the team
https://t.co/UX6YyWJRDk
What if small molecules could allow you to alter protein localization at will?
What if they could allow you to turn on specific transcription factors?
Our new manuscript reveals bifunctional molecules to do just that. 🧵
TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec.
We explore 4 intracellular and intercellular applications of TransitID:
- discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix)
- mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress
- discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery
- analysis of proteins that traffic intercellularly bw tumor cells & macrophages
Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor
https://t.co/yo4laOA14z
Check out this preprint on cubanes. Using a convenient approach to cyclobutadiene and copper photoredox catalysis, we are able to access bioisosteres of ortho-, meta-, and para-substituted benzenes for Med. Chem.
https://t.co/tKEy1oxWjP
Using super-resolution microscopy, we provide some insight into the spatial selectivity (labeling radius) of proximity labeling technologies. We also developed some new ones along the way. https://t.co/bFcF3zlKyK
Plus another @Merck collaboration with @MacMillan_Lab showcases how an additive screen can substantially broaden the scope of nickel/photoredox catalysis https://t.co/Q6tECOb1Yv
In @J_A_C_S, we report a platform for red-light photoproximity labeling as we move toward in vivo applications. Congrats to @BenitoBuksh, @MrSteveKnutson, @JamesO760, @CiaranSeath, collaborators from the Scholes Lab and @PlossLab, & the whole team! https://t.co/9aF6C4QvTb
We're hiring! We have several postdoc positions open for chemical biology research and proximity labeling in the MacMillan Lab. Experience in cell biology, biochemistry, molecular genetics, immunology, or related fields desired. DM us with your info and a CV/cover letter!
We are actively recruiting a postdoc interested in working on developing new chemical biology tools for protein interaction discovery. If interested, please email me at [email protected]!
https://t.co/smnCJo39gj