James Oakley

Principal scientist, chemoproteomics @Pfizer. Proximity labeling
PhD @PrincetonChem, NSF fellow

[James Oakley @JamesO760]

Really nice work showcasing how tuning chemical reactivity can improve spatial resolution for proximity labeling. Super cool.

Directed Evolution of APOX for Proximity Labeling Using Phenols with High Redox Potentials | ChemRxiv - https://t.co/jsf62mECvx

[Steve Knutson @MrSteveKnutson]

We're entering into a golden age in proximity labeling, and photocatalysis is a major new driver for uncovering biological mechanisms. Check out our review (@BenitoBuksh @HuthSean @DanielleMorganC ) of the latest developments out now in @CellChemBiol!

[bryan dickinson @chembioBryan]

Our new proximity labeling technology is now published!!

This new subgroup is doing some amazing work - more to come!

https://t.co/GpQO7hDREY

[Rovis Group @RovisGroup]

New year, new proximity labeling protocol! We can use red light to access trifluoromethyl carbenes from diazos for proximity labeling. Congrats to @crabaneros and @stavkariofillis for a great paper in collaboration with Merck!

https://t.co/KCjAul1cCi

[David R. Liu @davidrliu]

In a major milestone for science, for medicine, and for patients, the world’s first CRISPR drug has been approved in the UK.
“A new treatment for sickle-cell disease and transfusion-dependent β-thalassemia has been authorised by the Medicines and Healthcare products Regulatory Agency (MHRA) for patients aged 12 and over after a rigorous assessment of its safety, quality and effectiveness.”
Congratulations to the many scientists, doctors, patients, and industry leaders who made this development possible.🍻
https://t.co/pUL956YsG3

[Jacob Geri @JacobGeri1]

The Geri Lab is recruiting postdoctoral scholars to develop photocatalytic proximity labeling and proteomics technologies. Chemical biologists and chemists who want to learn chemical biology are welcome!

https://t.co/smnCJo39gj

The view from our recently expanded NYC lab space:

[Daniel Geiszler @GeiszlerDaniel]

Very cool work mapping binding sites with amino acid precision! And it’s extra rewarding when I see people I know using FragPipe 🤗

[James Oakley @JamesO760]

@HuthSean @CiaranSeath @Merck and shoutout to fellow @CSUSM grad @GeiszlerDaniel for the awesome proteomic software we used for this work 😎

[James Oakley @JamesO760]

Excited to share our latest work on small molecule binding site ID. We demonstrate this application across 7 different protein-ligand pairs and in live cells. Really fun team effort with @HuthSean @CiaranSeath @Merck and the rest of the team
https://t.co/UX6YyWJRDk

[William Gibson @wgibson]

What if small molecules could allow you to alter protein localization at will?

What if they could allow you to turn on specific transcription factors?

Our new manuscript reveals bifunctional molecules to do just that. 🧵

[Alice Ting @aliceyting]

TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec.

We explore 4 intracellular and intercellular applications of TransitID:
- discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix)
- mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress
- discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery
- analysis of proteins that traffic intercellularly bw tumor cells & macrophages

Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor

https://t.co/yo4laOA14z

[James Oakley @JamesO760]

@robertiafe @CSUSM @calstate so great to see this! congrats

[MacMillan Group @MacMillan_Lab]

Check out this preprint on cubanes. Using a convenient approach to cyclobutadiene and copper photoredox catalysis, we are able to access bioisosteres of ortho-, meta-, and para-substituted benzenes for Med. Chem.

https://t.co/tKEy1oxWjP

[NH BAE @ghostbnh]

https://t.co/fD4EMjzP0y https://t.co/DZkOPFwf56

[Princeton Chemistry @PrincetonChem]

Great collab between @PlossLab @PrincetonMolBio and @MacMillan_Lab @PrincetonChem, ID’ing 8 novel receptors for #COVID19 on the human cell, including 4 important for viral entry. Congrats to all. Story, link to @J_A_C_S paper: https://t.co/iVoWLfq8Y1

[James Oakley @JamesO760]

Using super-resolution microscopy, we provide some insight into the spatial selectivity (labeling radius) of proximity labeling technologies. We also developed some new ones along the way. https://t.co/bFcF3zlKyK

[Jake Yeston @JakeYeston]

Plus another @Merck collaboration with @MacMillan_Lab showcases how an additive screen can substantially broaden the scope of nickel/photoredox catalysis https://t.co/Q6tECOb1Yv

[James Oakley @JamesO760]

Excited to share this one. Great team effort.

[MacMillan Group @MacMillan_Lab]

In @J_A_C_S, we report a platform for red-light photoproximity labeling as we move toward in vivo applications. Congrats to @BenitoBuksh, @MrSteveKnutson, @JamesO760, @CiaranSeath, collaborators from the Scholes Lab and @PlossLab, & the whole team! https://t.co/9aF6C4QvTb

[MacMillan Group @MacMillan_Lab]

We're hiring! We have several postdoc positions open for chemical biology research and proximity labeling in the MacMillan Lab. Experience in cell biology, biochemistry, molecular genetics, immunology, or related fields desired. DM us with your info and a CV/cover letter!

[Jacob Geri @JacobGeri1]

We are actively recruiting a postdoc interested in working on developing new chemical biology tools for protein interaction discovery. If interested, please email me at [email protected]!

https://t.co/smnCJo39gj