Joseph Kim

Postdoc at UCSF | PhD from UW-Madison | Cryo-EM and its applications to drug discovery and neuroscience. Brunch, Sugar, Triathlons, Video Games, and Museums

[Joseph Kim @jykim35]

Me: *Minding my own business*

Liquid Nitrogen Tank Pressure Relief Valve: ALLOW ME TO SING YOU THE SONG OF MY PEOPLE.

[Joseph Kim @jykim35]

Good morning everyone, it’s a beautiful day to make a mistake!

[Joseph Kim @jykim35]

Well done Lauren! It was a privilege to work with you when I was at Madison!

[Lauren English @englishlauren2]

Very excited to share a preprint of my thesis work examining the form of the F-BAR proteins CIP4 and FBP17 in neuronal migration!

[Joseph Kim @jykim35]

Congratulations to you and the rest of your team Steve!

[MansoorLab @MansoorLabOHSU]

Happy to share a pre-print of some recent work.

This is our first time using bioRxiv. I suspect we will be using it more moving forward.

[Joseph Kim @jykim35]

Thank you to all the collaborators, co-authors, and mentors for all the help throughout this project! @JYang22638056, Dr. Josephine Mitchell, @englishlauren2, Dr. Sihui Yang, Tanner Tenpas, Professor Erik Dent, Professor Jill Wildonger, and my advisor @erwright73!

[Joseph Kim @jykim35]

I would like to announce that my work on cryo-ET on Drosophila neurons is now published on @MAMtheJournal! #Tomography #TeamTomo
https://t.co/6dopsqjCbt

[Joseph Kim @jykim35]

We tried so many different things to try to optimize this platform, and learned a lot of things along the way in regards to the difficulties in sample preparation for cellular cryo-ET (with special attention to neurons).

[Joseph Kim @jykim35]

While I wasn’t able to attend the last day cause I got ill, I just wanted to say it was a blast attending my first ever SfN conference here at DC!

It was great seeing lectures where cryo-EM and cryo-ET got a shoutout, and meeting so many new people and old friends too! #SfN2023

[Joseph Kim @jykim35]

@vikasnavaratna Yes! Cheryl’s was so good! Your stamp of approval tells me that I made a really good choice for my post-interview breakfast.

[Joseph Kim @jykim35]

I may have developed a strong liking for french toast this summer.

[Joseph Kim @jykim35]

Great work, Qixiang!

[Qixiang He 贺琦翔 @QixiangHe]

Happy to share our review of the human telomere C-strand fill-in mechanism. Appreciate the opportunity to be involved in the process and learn a lot from @cijilim and the revision. https://t.co/pA58ENSEr0

[Elliot Hershberg @ElliotHershberg]

Transferrin Receptor Targeting Chimeras (TransTACs) 🧬

The field of targeted protein degradation is going through a renaissance. The core idea: use a bifunctional molecule to bring a protein in proximity to a degrader.

The first wave (PROTACs) primarily used E3 ligases as the degrader. They ubiquitinate the protein target, labeling it for degradation.

These have primarily been small molecules that target intracellular proteins.

What about membrane proteins?

There have been a few new protein degrader technologies designed with the goal of degrading targets that reside in the membrane—namely AbTacs and LYTACs—but the field is still evolving quickly.

A new preprint from the @xinzhoulab—the first preprint from their group—introduces a new idea:

Create a bi-specific antibody that targets a protein-of-interest (POI), and the Transferrin receptor 1 (TfR1).

Why TfR1?

1. It's up-regulated in cancer cells, which need lots of iron to proliferate. This can help with cancer targeting.
2. It has an insane recycling rate from the membrane.

By insane, I mean:

"TfR1 exhibits an average internalization rate of 500 molecules per cell per second (!!!), making it one of the fastest internalizing receptors known."

So the thinking is that this fast recycling will help to pull lots of the POI into the cell for targeted degradation.

Some solid POC data for demonstrating the degradation of CAR receptors:

(Look at the lack of CAR present on the cell surface in v1.0 vs. the control.)

Very cool idea!

[nature @Nature]

Nature research paper: Rewiring cancer drivers to activate apoptosis https://t.co/KTP8oNMnF5

[Kelvin Lau 🧬🧪💎 @klausenhauser]

Now this is cool. CryoEM visualizes of an adverse drug interaction of an antiviral together with an anti arrhythmic in Cav1.1 and 1.3! Somehow I doubt this could’ve been predicted like this. https://t.co/26QIdlsSAy

[Joseph Kim @jykim35]

Hi! It me! Uh I think I do biological research though…

[Microscopy Society @MicroscopySoc]

Congratulations to Tuesday's #MM2023 Poster Winners!

[Joseph Kim @jykim35]

This was one Godzilla of a project. So glad that I can share this with the cryo-EM/cryo-ET community!

[Elizabeth Wright @erwright73]

🎊Check out @UWMadisonChem & @UWBiochem student @jykim35 outstanding work developing #Drosophila #neurons as a model #cryoET system! A true tour de force with cell culture, grid prep, micropatterning, etc! 🥳https://t.co/Jy6lgGWDRN

[Joseph Kim @jykim35]

Juan - The superstar Caulobacter flagella man!

[Elizabeth Wright @erwright73]

🎉 Super excited to see @UWBiochem & @UWMadBiophysics student @_Juan_C_Sanchez🥇 first-author paper! An amazing #cryoEM structural journey with 3! Caulobacter #flagella! Providing insight into flagellin packing & multi-flagellin filaments. More to come!🎉 https://t.co/DfiNYbwqG1

[YingYin @ying__yin]

Molecular basis of polyspecific drug and xenobiotic recognition by OCT1 and OCT2. The final version is out today on @NatureSMB
https://t.co/Usviz5FFWH

[Joseph Kim @jykim35]

I ran my first half marathon! Very happy I got to do this, thanks to everyone who came out to support me!