Kim Ritola

[Kim Ritola @KimRitola]

Neurotools is at SFN! Stop by exhibitor booth 1850 to see our custom viral vector offerings. I will be there till 1 today and tomorrow.

[Kim Ritola @KimRitola]

@MartinBohlen1 @uncneurotools I am so happy for you guys, @MartinBohlen1 ! I love working with people who are as particular as I am. I can’t thank you enough for being down for whatever I throw your way. Can’t wait to hear about it!

[Kim Ritola @KimRitola]

Can someone please make it makes sense @UNCMedCenter, @UNC, @UNC_Hospitals.UNC Family Medicine (as well as UNC PT) is not a “clear pricing provider” for UNC’s employee health insurance.I have to pay extra to use the health care from the institution providing my health insurance.

[Kim Ritola @KimRitola]

@kaymtye If choose no surgery, I saw a paper on here showing healing without surgery as long as there was no cartilage damage and wearing a special brace. An Australian group…

[Kim Ritola @KimRitola]

@kaymtye I came back to action, no problem after the ACLs and a subsequent scoping, but Achilles has literally taken years. Running >30 miles/week and racing again at about 90% recovered now.

[Kim Ritola @KimRitola]

@kaymtye I could only answer once, but I have had hamstring graft for one and patellar for the other. Most important is the surgeon.

[Kim Ritola @KimRitola]

@varsitytutors Update! @varsitytutors says they will escalate to their “client experience team”. Hah! I know that crew. We have had more than one conversation that led nowhere. No one can “do” anything. Another trip around the circle begins.

[Kim Ritola @KimRitola]

Never ever ever use @varsitytutors. Since April we have been through 7 tutors, months without a new tutor assigned, and 100% lack of them caring. Cannot rate them low enough.

[Kim Ritola @KimRitola]

@varsitytutors Not meeting expectations is an understatement, at best. I will DM, but my expectations are extremely low after already spending hours on the phone with multiple representatives, none of whom seem can do anything but make empty promises.

[Kim Ritola @KimRitola]

@MarkZylka @LukensJohnR @NeuroCellPress Agree! So much to learn about the negative impact of known (or new to our knowledge) viruses. No doubt can be used to better understand the what and how. Not impossible there just might be some good associations too!

[Kim Ritola @KimRitola]

@miketadross We are writing up how to grow this with minimal recombination, but basically speed is king. I know, I know, ITRs. They will be OK! Hope@to have it on website by end of week. DM if can’t wait.

[Kim Ritola @KimRitola]

@miketadross Matters how you grow these constructs. If done wrong, already recombined in DNA and the virus prep just reflects that. If you are growing at 30 and storing plates/growths, you are not helping. Greater distance between lox sites really helps too.

[Nathan @Humanstein]

Probably gonna work itself out.

[Kim Ritola @KimRitola]

@ControllingLMNT @TessaMontague Want to try it? I’ll send some! HSV too.

[Kim Ritola @KimRitola]

@staci_bilbo @PhilpotLab @nanopharmNC @uncneuro We have a 5ul AAV capsid sample library with CAG-GFP so you can try a range of capsids in your specific system. Prep quality matters A LOT and can make or break a capsid. Can send specific capsid not in list or collab on new capsid library!

[Kim Ritola @KimRitola]

@uncneurotools

[Ben @PhilpotLab]

Need high quality AAV, lenti-, or rabies-virus for neuro applications? Look no further than the UNC Neuroscience Center and the BRAIN Initiative Viral Vector Core!!!
https://t.co/GHlYd7xbSE
@KimRitola @uncneuro

[Kim Ritola @KimRitola]

@SA_Diabetes @superkash @uncneurotools Can still get decent titers above that, but we alter the protocol a bit. Success up to 6.2kb so far!

[Kim Ritola @KimRitola]

@SA_Diabetes @superkash 4.7 kb should not cause any titer issues. Typically ok till around 5.2kb. @uncneurotools