UNC-NeuroTools

Custom viral vectors for Neuroscience. Supported by UNC Neuroscience Center and the BRAIN Initiative.

[UNC-NeuroTools @uncneurotools]

@VincentCostaPhd Thanks @VincentCostaPhd! Our work isn’t done till these viruses work for you. Glad they are, but if you ever have an issue, know our job isn’t done till it works. Thanks to the BRAIN Initiative for their critical U24 support.

[UNC-NeuroTools @uncneurotools]

@uncneurotools is looking for a tech! Join us to learn about viral vectors in gene therapy and neuroscience! https://t.co/34RflEsmdH

[UNC-NeuroTools @uncneurotools]

@FreglisPlethon https://t.co/epTrTSBKzZ

[UNC-NeuroTools @uncneurotools]

We can take on a few more labs to fine tune our logistics. Turn around time for AAVs is 3-4 weeks from when we receive your DNA to virus back in your hands. At just under $500/100ul for neuro applications, it’s a pretty sweet deal (thank the BRAIN Initiative).DM for more details!

[Kim Ritola @KimRitola]

@uncneurotools

[UNC-NeuroTools @uncneurotools]

@FreglisPlethon Yes, whatever payload you send us in the capsid of your choice! Please@just make sure the DNA is has intact ITRs, EF-free, and if purified by isopropynol ppt, you have done a EtOH ppt clean-up.

[UNC-NeuroTools @uncneurotools]

@SA_Diabetes @KimRitola @superkash We haven’t really seen a difference based on capsid. Expressed payload during virus production effects are greater.

[UNC-NeuroTools @uncneurotools]

Come see us at #SfN22! Join us Sunday 11/13 6:30-9pm at the BRAIN Initiative Alliance Social for Tools, Tech and Theory. https://t.co/yDhO14g2av #studyBRAIN

[UNC-NeuroTools @uncneurotools]

@k_pietersz @plasmidsaurus Works well for finding deletions outside the traditional restriction digest sites. Also very handy for looking at the homogeneity of your entire expression construct. Our prep will reflect the DNA quality. Bad in = bad out.

[UNC-NeuroTools @uncneurotools]

Welcome to UNC NeuroTools! Formal request system to go live around June 9. Until then, get your EF DNA ready, QC constructs/ITRs (@plasmidsaurus) and register for an iLab account (for billing only-can do PO, check, or CC). Questions? Let's hear/share them....

[UNC-NeuroTools @uncneurotools]

Thanks neuro-community! Our Beta testers are set. Stay tuned for the next phase!

[UNC-NeuroTools @uncneurotools]

@Mayank_Verma_ Our cut-off is E12, but we typically get E13 across serotypes. If you need a specific titer we can adjust our protocol. We know some experiments require high titer/purity regardless of expression construct impact on titer.

[UNC-NeuroTools @uncneurotools]

@Andy__Osborne You just need to sign up for billing, order some viruses, and give us feedback on the process (mostly what's a pain or what's missing) including how well the viruses worked.

[UNC-NeuroTools @uncneurotools]

We are looking for about 20 external to UNC volunteers to Beta test our billing and virus request systems. Please DM us if you are interested!

[UNC-NeuroTools @uncneurotools]

@vertigologist @MartinBohlen1 Yes, just be sure to know the customs requirements beforehand so nothing gets held up!

[UNC-NeuroTools @uncneurotools]

@VincentCostaPhd @btaylorblake the GOAT.

[UNC-NeuroTools @uncneurotools]

@JustinGEnglish @nanopharmNC Yes, we specialize in custom!

[UNC-NeuroTools @uncneurotools]

@agetliang Hi Yajie! Happy to hear from you! Hope MD is treating you well. Send me your email and I will get you the info! Thanks!

[UNC-NeuroTools @uncneurotools]

@GattoGraziana @KimRitola @uncneuro Customs can make it tricky. Do you have a customs agent you usually deal with? Not a bad idea to figure out exactly what they need before hand:)

[UNC-NeuroTools @uncneurotools]

What makes our AAVs different? They are created with neuroscience applications in mind. Smaller volumes (100-500ul), custom capsid choices, and personalized vector production protocols to attain titer and purity necessary for results in YOUR experiment.

[UNC-NeuroTools @uncneurotools]

@superkash @KimRitola @uncneuro I am a big fan of rabies!