I highly recommend "How to read (single-cell RNA-seq) PCA plots" (by @vallens): https://t.co/Co3A47SZTR #gi2019
He notes that "A particular danger [in interpreting T-shapes] is that it is tempting to interpret this as a bifurcation in the data." (it almost never is). #gi2019
This is amazing and may be the death of ChIP-seq. Single cell CUT&RUN, as in just one cell! Profiling of pluripotency factors in individual stem cells and early embryos https://t.co/9stjsFvgtN
Repeat after me: A reviewers role is POLITELY point out flaws in manuscripts and provide HELPFUL suggestions with the goal of helping authors IMPROVE their manuscript. The goal is not to find some random reason to reject the paper. And definitely not a license to be an ahole.