Marcel Müller

Fluorescence Microscopy, lead developer of the project for Structured Illumination. Bielefeld University. Work here, more @LightAndScience

[Marcel Müller @mueller_physics]

Blue-print for an SLM-based SIM system, made (almost) completely from readily available components:
https://t.co/4uS3PSYPE5
By Robin Van den Eynde at @DedeckerLab

[Dedecker Lab (dedeckerlab.bsky.social) @DedeckerLab]

Doing multicolor (single-molecule) imaging? Tired of waiting for each color?
Want simultaneous imaging with full field of view but without the multicamera hassle?
Take a look at our 'Circulator'! 🔥💪
https://t.co/MjflFNdSGl. Very excited so please RT! (1 of 5)
#microscopy

[Micron Bioimaging Facility @MicronOxford]

Applications are now open for our advanced #SIM practical course! If you are an #imagingscientist working in a #corefacility or research group supporting or soon to be supporting SIM projects, then this is the course for you! More info here https://t.co/tF8i29TMJL 🔬

[Andrea Bassi @AndreaBassi78]

After a long programming week we released a new @napari_imaging plugin for #StructuredIlluminationMicroscopy, #gpu accelerated with #PyTorch. Drop your raw images in #napari and let us know if you manage to reconstruct your #SuperResolution images.
https://t.co/PMG6b51y4w

[Marcel Müller @mueller_physics]

Together with the setup's blue-prints by @NanoSand_Alice
https://t.co/rdQBJN17EX
and the simulation framework
https://t.co/Mefes9eeBT
there's now quite a bit of detail on how to use DMDs in SIM applications.

[Marcel Müller @mueller_physics]

Our third (and for now last...) paper on DMD-SIM, where we use temperature control of a diode laser to tweak its wavelength to match the DMD's blazed grating.
https://t.co/YPLfehI0O4

[Marcel Müller @mueller_physics]

It is an alternative to the very nice approach of P. Brown et al. (@DougPShepherd, @ptbrown1729), where they use a voice coil mirror to slightly adjust the angle instead of shifting the laser wavelength:
https://t.co/6qou9Okc3P

[Marcel Müller @mueller_physics]

@DougPShepherd @RetoPaul @christlet @NikonInst Currently not, though I wonder why... I'll talk to my collaborators next week and see if there's any reason to not share the code on github and/or Zenodo, where the data already is available.

[Marcel Müller @mueller_physics]

@RetoPaul @christlet @NikonInst This compares two different network layouts (U-NET and RED-NET), and running the full reconstruction vs. only using the network for denoising and artifact removal after a "classic" reconstruction.
Still hope to find someone to bundle this into a ready-to-use Fiji plugin.

[Marcel Müller @mueller_physics]

@RetoPaul @christlet @NikonInst We use the coarse(r) patterns to fill the missing cone in 2D SIM, but in a collaboration we've also tried enhancing SIM data with neural networks lately, and see quite promising results on low-SNR data.
https://t.co/MAbJqkbBJQ

[Hana Valenta (hanavalenta.bsky.social) @HanaValenta]

So happy to see this paper out! 😊Together with @SHugelier @mueller_physics, @Sam_Duwe and @DedeckerLab we present a simple method to efficiently separate spectrally overlapping FPs! 🔬 https://t.co/pQhQOyvZIj

[Marcel Müller @mueller_physics]

@heiseonline @OpenUc2 @beniroquai

[heise online @heiseonline]

heise+ | UC2-Modulbaukasten: Optik‑ und Mikroskopie-System aus dem 3D-Drucker https://t.co/l4EdM1voRC #heiseplus #Baukasten

[Marcel Müller @mueller_physics]

@HenriquesLab I'd be happy with my current position (senior postdoc/staff scientist) being permanent. But since that's not possible, I pursue both an academic path (if it works out) or joining the industry. I don't have a strong preference which one it will be, currently doing both.

[Marcel Müller @mueller_physics]

@beniroquai @mindnumbed @LaineBioImaging @HenriquesLab @DrBrianPatton @RetoPaul @Hannah_SuperRes @AlliedVisionTec @flir Also, IIRC, some of those Sony sensors can be found in cameras aimed at astronomy, with a cooling solution already designed and built in. Never got one of those to test though.

[Marcel Müller @mueller_physics]

@beniroquai @mindnumbed @LaineBioImaging @HenriquesLab @DrBrianPatton @RetoPaul @Hannah_SuperRes @AlliedVisionTec @flir Temperature is a good question. Qualitatively yes, cooling will help a lot with noise, quantitatively I think we might have even tested this (Peltier, cooling the housing), I'll try to find whoever might have that data.

[Marcel Müller @mueller_physics]

@mindnumbed @beniroquai @LaineBioImaging @HenriquesLab @DrBrianPatton @RetoPaul @Hannah_SuperRes We're currently looking into how much calibrating these cameras ourselves (per pixel gain curves, etc.) might improve performance. Though the project is a bit on the side burner currently.

[Marcel Müller @mueller_physics]

@mindnumbed @beniroquai @LaineBioImaging @HenriquesLab @DrBrianPatton @RetoPaul @Hannah_SuperRes For sCMOS, manufacturer calibration is a really big effect, but for the more cost-effective machine vision type cameras we never looked at this, and I'd also not expect there to be a significant difference.

[Dedecker Lab (dedeckerlab.bsky.social) @DedeckerLab]

Pleasure to see paper of @Robin_VdE et co-authors as the top download in Biomedical Optics Express! 🥳
Find full paper: https://t.co/pMHVGMDwjv
and SI here:
https://t.co/K6LBDHllko

[Marcel Müller @mueller_physics]

Robin is also on Twitter here: @Robin_VdE

[Marcel Müller @mueller_physics]

Blue-print for an SLM-based SIM system, made (almost) completely from readily available components:
https://t.co/4uS3PSYPE5
By Robin Van den Eynde at @DedeckerLab

[Marcel Müller @mueller_physics]

@paravatar @DedeckerLab No, unfortunately, the links in the PDF do not seem to point to the latest SI. That should be fixed soon, until then, here's a direct link:
https://t.co/7uGpElXpCx
Among the documents there is a parts list as an Excel file.