Micha Müller

PhD student in Tanenbaum lab @_Hubrecht, former member of @pelkmanslab at UZH

[Micha Müller @muellermicha_]

Very happy to share our preprint on visualizing the life cycle of Influenza viruses using single-molecule imaging!🥳 We developed two techniques to visualize infections of unmodified influenza viruses in live cells from endosomal release to budding of new viruses. Long thread ⬇️

[Lucas Kissling @KisslingLucas]

Excited to share our paper in @ScienceTM!
Together with @Francesca_Pietr as co-first author, we used in vivo prime editing to correct a pathogenic SCN1A mutation in a GEFS+ mouse model.
Huge thanks to all co-authors for their work!
https://t.co/hmklFBYuaJ

[Ina Sonnen @SonnenKatharina]

How do embryos ensure precise tissue patterning? It’s all about timing cell divisions! Our new preprint reveals how cell proliferation syncs with signalling oscillations to regulate precision of somite formation and growth. Check the full story: https://t.co/ZBxdOXbXGB

[Marvin Tanenbaum @MarvinTanenbaum]

Our paper on Stopless-ORF Circular RNAs (socRNAs) is now out in Cell. By high-res tracking and comparing translation by either single or multiple ribosomes, we find that ribosomes cooperate to overcome pausing to ensure fast and efficient translation

https://t.co/Oyw32LlbxM

[Jeroen van den Berg @jervdberg]

Happy to share this concise review and outlook for single-cell sequencing methods to unravel cell biology of the nucleus! Big shoutout to my friend and former colleague Peter Zeller for sharing the work!
https://t.co/EQNitlrMeX

[Marvin Tanenbaum @MarvinTanenbaum]

Our new paper is out: "Mapping the complete influenza A virus infection cycle through single vRNP imaging". Combining single-molecule imaging approaches with in situ transcriptomics, we identify many non-canonical infection pathways.

https://t.co/3xV3Kaw7vL

Movie: viral entry

[Micha Müller @muellermicha_]

This was a big team effort by the x-less Huib, @jschoko98, @BaarsMatthijs and Jakob in @MarvinTanenbaum lab. Also, a great collaboration with RonFouchier and @nella_dost & @HansClevers on the patient samples and airway organoids!

[Micha Müller @muellermicha_]

Influenza viruses are segmented, (-)RNA viruses that, like other (-)RNA viruses, have their genomes encapsidated by nucleoproteins (NP). We exploited this characteristic to visualize single, unmodified IAV genomes by expressing a fluorescently labelled nanobody that binds to NP.

[Micha Müller @muellermicha_]

We envision that the tools described in this manuscript open up new avenues to study IAV biology in unprecedented detail. Conceptually, with virus-specific modifications our imaging systems are even broadly applicable to many different (-)RNA viruses.

[Micha Müller @muellermicha_]

Link to preprint:
https://t.co/1j1GhSvxZE

[Micha Müller @muellermicha_]

Very happy to share our preprint on visualizing the life cycle of Influenza viruses using single-molecule imaging!🥳 We developed two techniques to visualize infections of unmodified influenza viruses in live cells from endosomal release to budding of new viruses. Long thread ⬇️