Christoph Thiele

Juggling with nanometres, nanoseconds and nanomoles.

[AlexNevs @OleksiiNevskyi]

🚨🎉Paper alert! Finally, our paper about the combination of ISM and SMLM is out! #SMLM #ISM #FLIM Many thanks to all contributing authors ⁦@NielsRadmacher⁩, Ignacio Gallea, Ingo Gregor, ⁦@nanofein⁩, Silvio Rizzoli and Jörg Enderlein. https://t.co/j2Pop4JpTB

[Eli Slenders @EliSlenders]

Excited to introduce ISM-FLUX, our new #MINFLUX-based technology! Achieve sub-10 nm localization uncertainty on single molecules using a straightforward #confocal-based setup with an array detector. 1/6
https://t.co/cScfkxIBSz

[Vicidomini Lab @VicidominiLab]

PhD Opportunity in Advanced Microscopy. If you're passionate about building innovative instruments and thriving in a hands-on lab environment, join our lab @IITalk. DM for info. Please RT.
#PhDPosition #fluorescence #photonresolvedmicroscopy #STEM
https://t.co/vC5SoxSAt8

[Nature Photonics @NaturePhotonics]

New #OpenAccess article online: Single-protein optical holography.

https://t.co/S93v5hwXWQ

[Chia-Lung Hsieh @ChiaLungHsieh]

I contributed a commentary in Nature Photonics on Philipp Kukura's groundbreaking study on single-protein optical holography. I am looking forward to a wave of innovative research that will enhance the sensitivity and precision of holographic microscopy. https://t.co/qkWl7viPHw

[Christoph Thiele @nanofein]

@ChiaLungHsieh Thank you for your perspective on our work! I really enjoyed reading your take. The outlook on long-term characterisation of a single proteins is very exciting.

[AlexNevs @OleksiiNevskyi]

I've always wanted to delve into virus imaging.🦠🔬 Please check out our newest preprint, where we introduce the use of the non-human-infecting virus, bacteriophage T4, as an effective and straightforward bio-ruler for 3D super-resolution imaging.📏 https://t.co/So1jo3F8xU

[Roman Tsukanov 🇺🇦 @RomanTsukanov]

📢 Excited to share our new preprint "Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT"! https://t.co/CPVTMYjYdv
#DNAPAINT #MIETPAINT #FLPAINT #SRM #MIET #FLIM @uniGoettingen
A short 🧵👇

[Christoph Thiele @nanofein]

@ghosharindam_92 Thank you Aridam! Looking forward to finally meet again in person at FOM2024 next week.

[Christoph Thiele @nanofein]

I had to share these videos. To see how we get the polarizability and scattering phase of single proteins, head over to @NaturePhotonics: https://t.co/cAEjRIX2aV 5/5

[Christoph Thiele @nanofein]

We can use the phase information to shift the focus in post-processing. No defocussed measurements anymore! For this measurement, the best focus is around +150 nm. 4/5

[Christoph Thiele @nanofein]

We create four phase-shifted channels (0, π/2, π, 3π/2) which are combined to get phase and amplitude of the scattering field. Here, we are moving a mirror to demonstate that with gold nano particles. 3/5

[Christoph Thiele @nanofein]

By using a Mach-Zehnder type interferometer, we can illuminate the sample in total internal reflection and get full control over the reference and scattering field. Advantages of this design are high speed and light throughput. 2/5

[Christoph Thiele @nanofein]

My first paper at @OxfordChemistry is out in @NaturePhotonics: Single-protein optical holography! Big thanks to Emanuel Pfitzner and Philipp Kukura. We improved the sensitivity of optical holography by orders of magnitude to detect single proteins. 1/5 https://t.co/cAEjRIX2aV

[AlexNevs @OleksiiNevskyi]

MiFoBio is approaching! I'm looking forward to nice talks and interesting discussions. If you are interested in Metal-Induced Energy Transfer and/or FluoLifetime Single Molecule Localization Microscopy, please come to our workshop or simply let's meet there. #MiFoBio2023

[AlexNevs @OleksiiNevskyi]

Our new preprint is out. FL-SMLM + ISM =💪Check it here👉Doubling the resolution of single-molecule localization microscopy with image scanning microscopy https://t.co/MsIOpSpxiz

[Arindam Ghosh @ghosharindam_92]

Preprint peer-reviewed and published now. Check out the work where we use graphene-induced energy transfer (GIET) to quantify nanoscale cholesterol-induced thickness variations in lipid membranes with sub-nm sensitivity. @TaoChen77217349 @JoergEnderlein
https://t.co/Wr5kAqD7qg

[Nano Letters @NanoLetters]

Most Read in the past 30 days: "Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis" by Oleksiievets, Mathew,Thiele, Gallea, Nevskyi, Gregor, Weber, Tsukanov, & @JoergEnderlein 
https://t.co/OJfCijSzkW

[Roman Tsukanov 🇺🇦 @RomanTsukanov]

Our recent story on wide-field and confocal Fluorescence Lifetime SMLM (FL-SMLM) is on the cover of @NanoLetters! Congratulations to all authors and big thanks to Alexey Chizhik @ChizhikAlexey for the great science art!
@ACS4Authors , #MyACSCover

[Fernando Stefani Lab @FerStefaniLab]

Do you have a confocal microscope in your lab? Then you can perform 1-nm single-molecule localizations in it with only a few modifications. We present #RASTMIN, an alternative to #MINFLUX that can be easily implemented in existing confocal setups. 1/6
https://t.co/s3z1lAFFte