Human phased T2T (20 T2T chromosomes + 9 further scaffolds) assembly from standard ligation library + 10X PoreC on a single PromethION flowcell (the unreleased high output chemistry)
#Nanoporeconf
Incredible few days at the Nanopore Community Meeting. Thank you to @nanopore for inviting me to talk about our work looking into base-pair level chromatin accessibility @nickdlowens#nanoporeconf
Dorado v0.8.0 #ncm24 release!
- v5.0 DNA transformer model performance improved for A100/H100 via kernel fusing, quantisation and leveraging i8 tensor cores.
- v5.1 RNA models with updated mod calling for m6A, pseU, and brand new calling of Inosine and m5C.
- Improved dorado correct performance, stability and flexibility.
- Introduces per-barcode configuration for poly(A) estimation with interrupted tails.
#nanoporeconf
https://t.co/13RCK9UI4d
Incredible presentation from Noah Bryan (a high school student!!!) on the detection of contaminated drinking water. And a clever rhyming title too! #nanoporeconf
Published today in @Nature, we describe an approach for single-molecule protein reading on @nanopore arrays. By utilizing ClpX unfoldase to ratchet proteins through a CsgG nanopore, we achieved single-amino-acid sensitivity. https://t.co/ZTFNZkwpcj
Edlib sequence aligner, https://t.co/MjKazNVExU, has more than 500 starts now. Kudus to @MartinSosic, who did this for his master's thesis and has continued to improve it over the years!
@colindaven @AW_NGS @krannich479 @keygene @nanopore@msikic So far we also tried haploid honeybee and tetraploid coffee each using a single flowcell worth of data. Standard read length not ultra-long and no pore-c / parental data.
@colindaven @AW_NGS @krannich479 @keygene @nanopore@msikic Hi Colin, if you are interested we have HG002 data (2 ULK flowcells) available here: https://t.co/SsRioOr2w2 including instructions on how we ran error correction and subsequence T2T assembly.
@lh3lh3@AlbertVilella@msikic @Dorylophile 's data after dark talk (which should be available on demand soon) has some more result for non-human and LSK assemblies.
Welcome to London ! At #nanoporeconf this year we will be live sequencing 10 Endangered and Critically Endangered Species to coincide with 10 years of London Calling. All supported through our https://t.co/YAahK2Wz2a programme. Please come speak to us about it @nanopore
Check out the data from this experimental extremely high-accuracy, ultra-long sequencing kit here https://t.co/egZVnPmD5S
The numbers from this tech update are incredible.