Sarah Mangiameli

Postdoc @broadinstitute working on new spatial genomics technologies in the @insitubiology lab

[Sarah Mangiameli @s_mangiameli]

We also thank @broadinstitute, @HSCRB , and @GRO_Broad for making this possible!

[Sarah Mangiameli @s_mangiameli]

Photoselective Sequencing is out in @naturemethods! This was a collaborative effort between the @insitubiology and @JD_Buenrostro labs, and I couldn't have done it without my co-authors @haiqi_chen, @H3K4me1 ,@juliedobkin_ , and @DLesman!

https://t.co/Sj5D93zW6m

[Sarah Mangiameli @s_mangiameli]

We are also grateful to our reviewers who helped us improve the manuscript - we have added several new experiments to better quantify the sensitivity and signal-to-noise ratio of our method!

[Jackson Weir @jacksonweir4]

Introducing Slide-tags: our new technology for truly single-cell, multi-modal spatial genomics!

Had a blast working alongside @AndyRusss, @Naeemnadaff, @macosko, @insitubiology, and the rest of our team on this project!

https://t.co/WXav5awW9u

1/9

[Andy Russell @AndyRusss]

Single-cell or spatial?

Our new technology - Slide-tags - allows both in the same experiment, enabling true single-cell multi-modal spatial genomics

➡️ https://t.co/i1m5T4bEme

[Sophia Liu @immunoliugy]

Presenting Slide-TCR-seq: our method for measuring the transcriptomes of cells & T cell receptor (TCR) sequences in situ!

This collaboration w/ Bryan Iorgulescu, Shuqiang Li, Catherine Wu, and @insitubiology led to some cool findings...

https://t.co/bamg5yCToX
1/8

[Sarah Mangiameli @s_mangiameli]

We are also grateful for support from @broadinstitute and @HSCRB. Check out the preprint for more details!

[Sarah Mangiameli @s_mangiameli]

Sharing Photoselective Sequencing (PSS), a new method for genomic and epigenomic measurements within spatial regions of interest.

Thanks to the whole team @haiqi_chen, @H3K4me1, @juliedobkin_, @DLesman, @JD_Buenrostro, and Fei Chen.

https://t.co/N2UrSGoPtQ

(1/9).

[Sarah Mangiameli @s_mangiameli]

We are especially excited to see PSS applied in subcellular regions. We selectively sequenced DNA at the nuclear periphery (key organizing region). We compare the results to Lamin B1 ChIP-seq data to identify general features of DNA sequences that localize to this region (9/9).

[Sarah Mangiameli @s_mangiameli]

Congrats @infectiousdose, so excited for you!!

[Houra Merrikh @merrikh_houra]

🍾 Proud of my postdoc Kevin Lang! He is starting his independent group June 2021 at the world-renowned Institute Curie @institut_curie, studying DNA replication during host-pathogen interactions. Can’t wait for exciting discoveries from the Lang lab! Congrats @infectiousdose! 🍾