Savage Lab

Account managed by grad students and postdocs 🤙 @UCBerkeley @berkeleyMCB @igisci @HHMINEWS

[Savage Lab @SavageCatsOnly]

Check out our new preprint on base editors and the thread below!

[Maria Lukarska @MariaLukarska]

I’m happy to share our pre-print exploring the activity and specificity trade-offs in adenine base editors. Thanks to my amazing co-authors @loltrogge, Hunter Nisonoff and all the other contributors @CynthiaTerrace @jlistgarten @dfsavage @SavageCatsOnly https://t.co/pbIGKGqHWf 🧵

[Scribe Therapeutics @scribetx]

Introducing $SCTX. As a newly #NasdaqListed company, we’re advancing our pipeline of purpose-built #CRISPR-based genetic medicines for cardiovascular disease and other common disorders.

[Maria Lukarska @MariaLukarska]

I’m happy to share our pre-print exploring the activity and specificity trade-offs in adenine base editors. Thanks to my amazing co-authors @loltrogge, Hunter Nisonoff and all the other contributors @CynthiaTerrace @jlistgarten @dfsavage @SavageCatsOnly https://t.co/pbIGKGqHWf 🧵

[Evan Groover @evan_groover]

Plants are our most scalable technology for managing atmospheric CO₂. Can we CRISPR them to capture it faster? 🌱

Our review: "Genome engineering of plant photosynthesis for carbon sequestration" is out now. With co-first author @florazhiqiwang + the Niyogi, Savage (@SavageCatsOnly), and Lemaux labs at @igisci @PMB_Berkeley.

Paper link below:

[Savage Lab @SavageCatsOnly]

Congrats to co-first authors @evan_groover and @ding_david and our talented team of collaborators, and many thanks to Christine Queitsch, @RNAnerd, and Jen Sheen for protoplast wisdom. We look forward to translating what we learned to stable plants!
https://t.co/ixTvTYilcL

[Savage Lab @SavageCatsOnly]

Can CRISPR edits enable precise tuning of plant gene expression? We think: yes.

In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.

[Savage Lab @SavageCatsOnly]

We show that genomic language models trained on plant genomes capture motif syntax and modestly predict the effects of hypomorphic mutations, but not hypermorphic ones. This suggests to us that problem-specific datasets like this will be essential to realize their promise.

[Nature Biotechnology @NatureBiotech]

Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression https://t.co/cVlQX7LEHS

[Nature Biotechnology @NatureBiotech]

Engineered TnpB genome editors for plants and human cells identified by ribonucleoprotein mutational scanning https://t.co/DH4Jpt165J

[Savage Lab @SavageCatsOnly]

Together, these findings showcase the potential of engineered TnpB editors delivered via viral vectors for efficient, heritable genome editing in plants 🧬.
(7/7)

[Savage Lab @SavageCatsOnly]

New from Ugrappa Nagalakshmi and @rodriguezje1991 🌱✂️– Gene editing in plants just got a lot easier! Using engineered TnpB, they demonstrate high-efficiency, heritable, transgene-free editing in plants. (1/7)

[Savage Lab @SavageCatsOnly]

They demonstrated enhanced activity of two engineered variants, eTnpBc (TnpB-KYLI) and eTnpBe (TnpB-VGIRL), across multiple plant species (N. benthamiana, rice🌾, and pepper🌶️).(read more: https://t.co/K2qgwKITwh)
(6/7)