Shiny Maity

Graduate Student #songihanlab Department of Chemistry #ucsantabarbara #biophysics #northwestern

[Han Lab 🧲 @ Northwestern University @songihanlab]

New Han Lab Paper Alert! Hydraulic Activation of the AsLOV2 photoreceptor https://t.co/zeUrefUafR

[Shiny Maity @shiny_maity]

RT @songihanlab: Congrats to @shiny_maity , Martyna, and from our group for receiving various awards for their research presentations at th…

[Global NMR Discussion Meetings @global_nmr]

Please RT! We are thrilled to announce the winners of @global_nmr Online Conference 2024 #GlobalnmrOC2024
These awards recognise outstanding science by students and postdoc researchers 🎉 Congratulations all!

We want to thank all the participants, our sponsors and the judges.

[Shiny Maity @shiny_maity]

@fscottdnp @songihanlab @SpinLovett @braddprice @fscottdnp Thank you!!

I have only tried PEG as a molecular crowder for my experiments. Trying ionic solutes would definitely be something interesting to look at!

The Overhauser DNP data was taken at a magnetic field of 0.35 Tesla and electron Larmor frequency of 9.8 GHz.

[Shiny Maity @shiny_maity]

Hydration water directly interacts with the protein interior or exterior and adopts different structures. The question we ask is whether the light-activated unfolding of AsLOV2 is driven by a thermodynamic action of water!
@songihanlab @SpinLovett @braddprice
#GlobalnmrOC2024

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr Thank you!!😊

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr PEG alone (no light) at high external pressure causes more population to shift at higher distance, compared to w/o PEG (no light) at high external pressure.

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr PEG alone (no light) at https://t.co/Q83iNRZh73. causes the protein to decrease the DEER distance a tiny bit when compared to w/o PEG (no light) at https://t.co/Q83iNRZh73.

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr When the pressure is from all sides though, like when we add just PEG (dark) at atmospheric pressure, it surrounds the protein from all sides and decreases the DEER distance a little compared to without PEG (dark) at atm.pressure.

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr Also, the pressure is not high enough to completely denature the protein. We have tested reversibility when we de-pressurize it.

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr The pressure is not coming from all sides. The setup is such that the pressure is exerted only from the top of the tube..thats why the deformation/compression of the helices leads to unfolding instead getting compressed into a ball.

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr I have not measured 1H NMR relaxation.

The 17O NMR peaks were assigned as wrap and bound based on the DFT calculations of water clusters of model systems. @lokeswarnmr is working on the DFT part currently!

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr The timescale for its light activation (from dark to light) is about 30 secs. And the dark state recovery takes around 1.5 min for the wild-type and 20 mins for the N414Q mutant.

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr DEER data shows that light activation decreases the P(r) population around 2-3nm and increases it around 4-5nm. In the dark state, the average distance is 2.5nm. However, due to C-terminal disorder upon light activation, we can only estimate the lit state distance range as 4-5nm.

[Shiny Maity @shiny_maity]

@asifequbal313 @global_nmr additionally, we also see, light+pressure the effect is additive: more population in the longer distance. And also by adding PEG (molecular crowder) we are locally increasing more pressure, which also causes more population to go to the longer distance conformation.

[Raj C: Free Our Union, Wear a Respirator @RajChaklashiya]

Happy to be participating in the online Global NMR Twitter Conference 2024! Here's a short video on our work at Han Lab to determine design principles for Electron Spin Cluster (ESC) DNP for signal enhancement at high field! https://t.co/zj2oTZCkHb #GlobalnmrOC2024 @songihanlab

[Chung-ta Han @chungtahan]

NMR can definitely play a big role in unveiling the secret of Alzheimer's disease! We utilize solution NMR to understand which part of the tau protein initiates its pinning to a tau amyloid fibril.
#GlobalnmrOC2024 @songihanlab #AlzheimersAwarenessMonth

[International EPR Society @EPR_ESR]

Congratulations to the IES poster prize winners from #Euromar2024: @shinymaity and @andreaeggeling. Pictured (L-R) are Oscar Millet, Euromar24 organiser (thank you!), Marina Bennati (IES President), Shiny, Andrea and Janet Lovett (IES Secretary).