Brad Price

UC Santa Barbara Physics PhD candidate studying high-field EPR on ITST's home-built 240 GHz spectrometer

[Shiny Maity @shiny_maity]

Hydration water directly interacts with the protein interior or exterior and adopts different structures. The question we ask is whether the light-activated unfolding of AsLOV2 is driven by a thermodynamic action of water!
@songihanlab @SpinLovett @braddprice
#GlobalnmrOC2024

[Brad Price @braddprice]

Happy to share a new, open-access paper published this week in Science Advances! https://t.co/HZmZ9frBm4

[Angewandte Chemie @angew_chem]

Triggered Functional Dynamics of AsLOV2 by Time-Resolved Electron Paramagnetic Resonance at High Magnetic Fields (Sherwin) @shiny_maity @braddprice @arnab_mukh3rjee @maxzwilson @SpinLovett @songihanlab https://t.co/oVR0nGdLk8

[bioRxiv Biophysics @biorxiv_biophys]

Triggered functional dynamics of AsLOV2 by time-resolved electron paramagnetic resonance at high magnetic fields https://t.co/7zSFFQsHcx #biorxiv_biophys

[Global NMR Discussion Meetings @global_nmr]

Cool poster by @braddprice on high magnetic field #EPR for studying protein dynamics!

@shiny_maity @songihanlab @arnab_mukh3rjee @SpinLovett @ucsantabarbara

https://t.co/orW64OFbsq

[Brad Price @braddprice]

Grateful for the award! This was a really nice conference.

[Global NMR Discussion Meetings @global_nmr]

Please RT. We are delighted to announce the prize winners of the @global_nmr Twitter Conference 2022 #GlobalnmrTC2022!

We thank ISMAR for funding these awards and we look forward to bringing this special event back to the #NMR community in 2023.

See🧵👇🏽

#NMRchat #chemtwitter

[Brad Price @braddprice]

@moxie9028 @shiny_maity @songihanlab @arnab_mukh3rjee @SpinLovett field-swept scan as a function of time for proper distance extraction.

My guess is that the simplest step to SNR improvement would be reducing protein tumbling through some sort of immobilization, maybe by attaching part of the protein to something more rigid. (2.5/2.5)

[Brad Price @braddprice]

My EPR poster submission for #GlobalnmrTC2022. We (@shiny_maity, @songihanlab, @arnab_mukh3rjee, @SpinLovett, and others) are working on filming protein motion in real time using EPR. Initial results on AsLOV2 -- a photoactive protein found in plants -- are quite promising!

[Brad Price @braddprice]

@moxie9028 @shiny_maity @songihanlab @arnab_mukh3rjee @SpinLovett though I think our main sensitivity concerns now come down to three things: (1) ensemble measurements and measuring dead or improperly labeled proteins (2) dipolar averaging due to fast tumbling in solution (3) only have a transient scan instead of a full (2/2.5)

[Brad Price @braddprice]

@adriandraney @shiny_maity @songihanlab @arnab_mukh3rjee @SpinLovett but @shiny_maity has been able to simplify and reduce preparation time so that it now only takes approximately one day's work. (2/2)

[Brad Price @braddprice]

@adriandraney @shiny_maity @songihanlab @arnab_mukh3rjee @SpinLovett Sorry for the slow response! There is a pretty well understood mechanism of undocking (here, for example: https://t.co/Fal2HZodxS). Doubly-labeled protein wasn't trivial to produce, but the enrichment process certainly helped. It is still relatively labor-intensive (1/2)

[Brad Price @braddprice]

@nmronline @shiny_maity @songihanlab @arnab_mukh3rjee @SpinLovett Thanks, I think it's interesting too! And may allow us to specifically address/modify protein sites to improve AsLOV2's (or other proteins) application as a micromachine!

[Global NMR Discussion Meetings @global_nmr]

Learn how @braddprice uses time-resolved EPR to film protein motions!

Abstract: https://t.co/GAss9b5sjm

#NMRchat #EPR @RSC_ESR #GlobalnmrTC2022