It is more important than ever to spend time talking to people about science.
Communicate its value, and its objectivity.
Talk to your neighbors, your bartenders, your barbers, your congressmen. Talk kindly to people whose political views differ from yours.
Protect science.
Climate progress is now truly up to us. With Trump in office, you can forget about action at the federal level. Our job is to figure out how to organize millions of people to keep driving the transition away from fossil fuels from the ground up.
Biden should go all out on climate for the next 75 days.
Declare a climate emergency. Get all the IRA money out the door. Permit new transmission lines. Stop new LNG exports. Ban drilling on public lands.
Make a mountain of progress for Trump to try and undo.
What does one's lab do if the @invitrogen precast gels we've been depending and waiting on since early April are now backordered until October? (if we don't have the traditional molds and gel electrophoresis equipment to fall back on)
@jrossibarra@klsywd @TLowePower I think Kelsey's right. It was something written into grad group contracts that allowed them to work an additional 25% on top of the ~50% fellowship employment. I think the rationale being that students would get teaching experience without being restricted to their fellowship.
@skryazhi @theboocock @UCSanDiego@UofCalifornia I'm not sure about other UC's but UC Davis Graduate Studies covers the pay difference for GRFP fellows for PIs. This also makes it so that students have access to "employee resources" that they weren't otherwise able to as external fellows. (I.e shipping, dry ice handling, etc)
Don't try to run a last minute PCR before your flight while leaving your brother unsupervised at home. Lesson learned.
Unrelated comment: Dish soap and detergent are two different things
*Learns how to troubleshoot PCR by making every mistake possible and then some with new/high quality reagents*
2 years of avoiding PCR later...
*Performs PCR with expired master mix, ancient primers, and questionably old PCR-grade H20 on low quality DNA*
Clear bands appear...
So a bunch of really big media outlets very, very catastrophically botched their coverage of the #IPCC report today hey
(it is fossil fuel propaganda and an outright lie to state that climate change "cannot be stopped". Burning fossil fuels causes it, not doing that stops it)
@caroline__daws @claire_willing @mykophile You could try prolonging the KOH step. I didn't figure out how to completely prevent the cell wall from staining, but the arbuscules and hyphae were very evident and had a darker stain so it wasn't problematic. I worked with wheat and soy mainly.
@caroline__daws @claire_willing @mykophile Maybe try different sections of the roots if you are expecting to see arbuscules. They might still be there! Sometimes the finer roots stain better
@caroline__daws @claire_willing @mykophile Yes, that does look like just the cell wall. (I've definitely had that happen) On the bright side, the roots are cleared really well and if there are AMF present you will definitely see darker stained structures in those regions.
Midway through my third year, big things happening:
1) Got the lab printer to finally connect to my laptop
2) Obtained my student ID card
#pandemiccohort