Simone Mortal

Postdoc @SLN_ICFO lab @icfo. @SISSA alumnus. Neuronal cell microscopy 🧠 Super-resolution #microscopy 🔬 #neuroscience #retina

[NG @nicogalvanetto]

Postdoc position open in my lab at @IBS_Grenoble @CNRS. Looking forward to doing great things together.
You can write me an email but eventually you'll have to formally apply here https://t.co/r03Q5ASsqc

[Oded Rechavi @OdedRechavi]

When you must come to the lab to run an experiment during the weekend and you're dying to go back home asap

[ICFO @ICFOnians]

📢 Save the date! Join us for MINDLAB, an interdisciplinary training on neuroscience and #photonics, exploring the brain from molecules to whole organisms.

🗓️ 4–7 Nov 2025
📍 ICFO

More details soon!

#MINDLAB #Photonics #Neuroscience #ICFO #SaveTheDate

[Simone Mortal @simone_mortal]

Fantastic time at #BER2025 in Granada @CanalUGR! Excellent international school in visual optics with an outstanding organizing committee. Always inspiring to discuss super-resolution and expansion microscopy in retina research. 🔬✨

#ExM @sedoptica

[Simone Mortal @simone_mortal]

Thanks @Thorlabs for this fabulous Christmas tree 🎄 🎁 🎁

[Marine Laporte @MarineLaporte8]

Nice first day at #spaom2024. Cool talks, great workshop #Leica and amazing city tour #toledo

[Marine Laporte @MarineLaporte8]

Thanks for this fantastic #spaom2024 meeting.
Very happy to connect with Spanish and Portuguese microscopy community and to get the chance to highlight how ‘cool’ #ExM can be

[Simone Mortal @simone_mortal]

Day 1. SPAOM 2024.

Excited to discuss expansion microscopy and its application to retinal research!

Great environment and community at #spaom2024 !!!

[SLN - ICFO @SLN_ICFO]

👨‍🔬 Dr. Simone Mortal, postdoctoral researcher

"Spatial protein localization in photoreceptor discs revealed by expansion microsopy and nanoscopy"

📆 DAY 1 – 20th nov 10.30h (Toledo room)

[Ali H. Shaib @AliHShaib]

Proud to announce that #ONE_microscopy is now published in @NatureBiotech. We now reveal that ONE enables the 3D reconstruction of single protein molecules from light microscopy. More here: https://t.co/Y5Fy7C8bM3

Silvio and I extend our immense gratitude to our collaborators @eboyden3, @LabSauer, @cppape, @RaduAricescu, @FelipeOpaz_o, @touteiro, @L_AJ_Alvarez, Tobias Moser, Ute Becherer, Brit Mollenhauer, Markus Müller, Chi Zhang and all the contributing scientists, especially @AbedShaib & @GSvilen.

Special thanks to our Editor, Dr. Bishop, and our reviewers, including Prof. Diaspro @AD1959, for their invaluable insights!

A heartfelt thank you to the scientific community for their interest, support and endorsement of our work.

[Vincent LOUVEL @louvel_vincent]

EM or fluorescence microscopy with 10X air objective & few fold of expansion ?🙃
(confocal, pixel size: 500 nm)
U2OS cell with Pan-Staining

[Simone Mortal @simone_mortal]

@JoshuaTran_guv That's true, methanol is usually present to stabilize formaldehyde. However, during the anchoring process, cross-linking is necessary to anchor the sample to the gel, so I would exclude the possibility that methanol could play a decisive role in this process.

[Simone Mortal @simone_mortal]

Last week, I was wondering why in all the expansion microscopy protocols I found, formaldehyde is used instead of paraformaldehyde for anchoring. PFA should be much more common and, in principle, it's the same. So, I tested it and, as expected, they look the same.
#ExM

[Simone Mortal @simone_mortal]

@samjlord Yes, I mean formaldehyde solution prepared from PFA. My point here is that in expansion microscopy protocols, PFA is always reported for fixing and FA for anchoring. However, there are no actual differences, and since PFA is more common, why not report only the PFA concentration?

[Simone Mortal @simone_mortal]

@manorlaboratory I think it's an impression due to the image compression by X/Twitter. I checked the signal intensity and sharpness of different images, and they look really similar.

[Simone Mortal @simone_mortal]

@FrancescoUlloa I tested both today; the yellow one (Sigma) expanded more, around 18% more than the other. However, it is definitely more fragile and less stable at high temperatures 👨‍🍳

[Simone Mortal @simone_mortal]

Different shades of sodium acrylate

It's always a surprise to discover what the new lot will look like 🤔🧐

#UExM #ExM

[Simone Mortal @simone_mortal]

@FrancescoUlloa The one the right it's Sigma, the one one left it's autoproduced.

[Simone Mortal @simone_mortal]

Amazing iU-ExM gel at @SLN_ICFO still fluorescent after one week in my backpack, definetly not bad 😁
Great staining of endoplasmic reticulum and tubulin @MarineLaporte8 and @louvel_vincent
#EMBOExM #UExM

[SLN - ICFO @SLN_ICFO]

Last week, Simone Mortal (@simone_mortal) attended the @EMBO Practical Course 'Ultrastructure Expansion Microscopy' at @embl.

#EMBOExM