Oliver Rackham

Synthetic biologist engineering mitochondria, RNA and new gene editing systems. NHMRC Investigator and Professor @CurtinUni @CurtinMRI and @TheKids_AU

[Oliver Rackham @TheRackhamLab]

The discovery of CRYAB as the binding partner of TANGO2 highlights the importance of intermediate filaments in metabolism and mitochondrial function and reveals the molecular basis of TANGO2 deficiency disorder (TDD)
https://t.co/7iTL7cYlpS
#metabolism
#mitochondria
@NatureComms

[ARC Centre of Excellence in Synthetic Biology @ARC_CoESB]

Sun, surf and scientific talks 😎

Centre Director Ian Paulsen and COO J-L Heylen joined CoESB researchers from our @uwanews and @CurtinUni nodes for their annual Theme 2 (Synthetic Organelles) Retreat on Rottnest Island in sunny WA 🌊☀️ @TheRackhamLab @FilipovskaLab @arc_gov_au

[Aleksandra Filipovska @FilipovskaLab]

It takes two to TANGO2!

TANGO2 binds the heat shock protein crystallin alpha B to prevent aggregation of the intermediate filament desmin and its loss models TANGO2-deficiency disorder

Many thanks to our team, collaborators and reviewers.

https://t.co/dQErSDSoUu

#metabolism

[Oliver Rackham @TheRackhamLab]

Ready to make breakthroughs in gene editing and mitochondrial biology in sunny Western Australia?
Join our vibrant team as a Research Associate or Research Fellow. Expertise in cryo-EM, cell imaging, or mammalian genetic screens is highly desirable
https://t.co/9PipVUF3Pt

[Aleksandra Filipovska @FilipovskaLab]

New structural and functional analyses show that FASTKD4 supports non-canonical RNA processing and MT-ND3 mRNA stability. Thanks to all authors for their contributions and the reviewers for their kind advice!

https://t.co/0eH1tbkKDA
#RNA
#mitochondria
#mtDNA

[Oliver Rackham @TheRackhamLab]

Destroyer turned protector - FASTKD4 uses it’s Vsr nuclease-like structure to protect the polyadenylated tail of the mitochondrial MT-ND3 mRNA

https://t.co/NMdOzqmu3o

#RNA
#mitochondria
#mtDNA

[Michael Lazarou @LazarouLab]

Update! We outline AIVE, an AI method for volume EM data. We show the benefits of AIVE for reconstructing your FIB-SEM data, reveal some fascinating cell biology, and define intrusions as a new form of mitochondrial contact.
https://t.co/nnxj2EB3s2

Muscle mito nanotunnels 1/3🧵

[Oliver Rackham @TheRackhamLab]

Better CRISPR base editors!
More efficient nicking of the unedited DNA strand improves the ability to edit hard to reach bases.
@ACSSynBio
https://t.co/M8Sw8vW12V

[Oliver Rackham @TheRackhamLab]

Organelles within mammalian cells are surprisingly interconnected, and lipids play a critical role

Check out: Interorganelle phospholipid communication, a house not so divided @Trends_Endo_Met to see more

https://t.co/UcmTw2UPFc

#metabolism
#mitochondria
#lipids
#Golgi
#ER

[Trends in Endocrinology & Metabolism @Trends_Endo_Met]

Online Now: Interorganelle phospholipid communication, a house not so divided https://t.co/FRo60hMaJA #trends #endocrinology #metabolism

[Oliver Rackham @TheRackhamLab]

Illuminating mitochondrial translation through mouse models
@hmg_journal
https://t.co/WiaIfbddvY
#mitochondria
#mtDNA

[Trends Cell Biology @TrendsCellBio]

Unique architectural features of mammalian mitochondrial protein synthesis https://t.co/Uu080Vbeii

[Aleksandra Filipovska @FilipovskaLab]

Unique architectural features of mammalian mitochondrial protein synthesis
30-day free access to our new review at: https://t.co/Mbsbic4SfK
Thanks to all the authors for their insightful contributions and our editor for their kind invitation and guidance.

[Oliver Rackham @TheRackhamLab]

High-fidelity Cas9 variants that prevent off-target cleavage typically reduce on-target activity. Adding TurboCas9 mutations to these enzymes can improve activity while maintaining off-target fidelity. @CellRepMethods
https://t.co/9AUclKZz81

[Cell Reports Methods @CellRepMethods]

Mutational rescue of the activity of high-fidelity Cas9 enzymes https://t.co/00hpp0qMlr

[Pascal Vos @pascal_d_vos]

Are you tired of slow gene editing and scattershot targeting?

Well no more, here we introduce HyperDriveCas9.
An engineered Cas9 variant that is both hyperactive and hyper accurate.

https://t.co/KIGZglw6AN

[Nature Cell Biology @NatureCellBio]

💫NEW: @FilipovskaLab & co use 3D cell reconstruction of focused ion beam scanning electron #microscopy data and #multiomics to show that ether-#lipid #metabolism regulates inter-organelle biogenesis and dynamics.
https://t.co/bJVMjtwrUL
https://t.co/I3PajGRSXm

[Aleksandra Filipovska @FilipovskaLab]

Quantitative volumetric analyses and morphological interactions supported by multi-omic profiling identify global organelle defects and mitochondrial dysfunction that can be rescued with ether lipids. https://t.co/nq0vjhVSvS
#FIBSEM #3D #mitochondria #organelles #lipids

[Oliver Rackham @TheRackhamLab]

Check out this elegant News and Views @NatureCellBio by Antentor Hinton Jr @phdgprotein86 and Andrea Marshall @DrDreNeuroWay, highlighting the importance of organelle interconnectivity:
https://t.co/zFCi76OmNG

[Oliver Rackham @TheRackhamLab]

“I am a part of all that I have met” – Ulysses; Alfred Tennyson

CRISPR screening, volumetric EM and multi-omics reveal the highly connected nature of organelles.

Defects in peroxisomes, Golgi and the ER converge on mitochondria via the lipidome.

https://t.co/QtkMmEx9CY