Thomas Leland

[Thomas Leland @tom1eland]

Invalid prompt: we've limited access to this content for safety reasons. This type of information may be used to benefit or to harm people. We are continuously refining our work in this area, and you can read more about our approach in our blog post (https://t.co/VaWYALLwCp) and Model Spec (https://t.co/vDVuStxv8b).

[Thomas Leland @tom1eland]

Imo the most straightforward way is to transfect a lot less plasmid. I ran into this trying to get both luminescence and transcript data with a luciferase plasmid. I tried many DNase conditions and none were good enough. The sensitivity of the two methods are too far apart to do both with the same sample & I gave up on qPCR. DpnI is an interesting idea, but I’d worry you run into the same issue.

[Thomas Leland @tom1eland]

There’s an easy argument that there should just be 5x fewer trainees, all of whom are paid 5x as much. Would fix a lot of problems & attract many more good minds. I’d guess that downstream we’d see more ideas good enough to get the attention of VCs, fund novel biology in the clinic, create jobs, etc

[Thomas Leland @tom1eland]

@parmita This is the best evidence that all of the detected life-molecules were not produced by life, imo

[Thomas Leland @tom1eland]

Someone should really just do this & tell us all if something shows up on an agarose gel or not

[Thomas Leland @tom1eland]

I’m still waiting @NASA

[Thomas Leland @tom1eland]

NASA, send me some and I’ll do a random hexamer PCR tomorrow. Or is the companion alien dna paper stuck in review at nature main?

[Thomas Leland @tom1eland]

@ATinyGreenCell It’s 100% not a waste of time & we’d all be better off if more scientists engaged with the public more often. I love how quickly it went to experimental design and choice of controls too

[Thomas Leland @tom1eland]

@ennucore For real though, I kinda love how wild this is. Glad you’re thinking of animals studies

[Thomas Leland @tom1eland]

@ennucore The animal in question

[Thomas Leland @tom1eland]

@jmschreiber91 @UMassGCB @IMPvienna I, for one, like the name. Any benefit over the R tfbstools library for finding motifs in a given sequence? I’ve been using that library to do your implementation #2, and it is very fast as well

[Thomas Leland @tom1eland]

@SynBio1 That assumes rare diseases don’t preclude becoming a centimillionaire in many ways.

But… if that rare centimillionaire is out there and reading this, I’m graduating soon from an AAV lab and do need something to do afterwards

[Thomas Leland @tom1eland]

@_inc0_ @ATinyGreenCell @ThatMrE Bioterrorism has happened, see anthrax etc. Per grok the soviets and US (pre ban) were making bioweapons. The Japanese used them on the Chinese in WW2. Barriers are lowering with new tools, AI being just one. Makes sense to be concerned & carefully consider how to manage

[Thomas Leland @tom1eland]

@realNathanCheng Hallmark gravity well

[Thomas Leland @tom1eland]

@CharlesMBrenner Healthy 90-95 year old trial population is probably the sweet spot for risk/reward, ease of recruitment, limited trial length

[Thomas Leland @tom1eland]

@Biotech2k1 @aditharun_ Viral gts can be dangerous at very high doses in very sick patients. They’re also the only effective option in many cases. Plenty of research is going into better dosing or aspects that could allow lower doses, and hopefully they will not always be exclusively for the very sick

[Thomas Leland @tom1eland]

PMCID: PMC386402

[Thomas Leland @tom1eland]

I know ChIP-seq is useful and all, but DNase footprinting must have been so much more satisfying