Our latest bioRxiv preprint characterizes our Arg-C Zero protease (available now at https://t.co/le1gAzp8bf 😏) What you may learn might surprise you!!1!! (ie. equivalent protein group ID's to Trypsin with half the peptide IDs)
#proteomics#teammasspsec
🔬 New bioRxiv preprint: Arg-C Zero, recombinant arginyl endopeptidase with >99% cleavage efficiency, proline-adjacent cleavage, Histone and labile PTMs analysis like ADP-ribosylation and comparable proteome depth to Trypsin.
https://t.co/cpWFzASQLG
#Proteomics#TeamMassSpec
1/5
Cell identity is written in the proteome, not in the DNA, and not always in the RNA. Out on bioRxiv today: The first cell type-resolved, MS-based proteomic atlas of the human body.
https://t.co/5RJ0nVoQ81
#TeamMassSpec: any independent data on dual-column LC creating subtle batch effects in LFQ proteomics?
Thinking column A/B intensity shifts, missingness, RT drift, or condition-column confounding. Vendor claims are nice; real-world datasets would be nicer.
Surprised to discover that Thermo Fisher appears to show a fake western blot for the validation of one of their p53 antibodies. I've added a diagram to show the very similar bands. This does not appear to be one of the "published figures", but their own internal data.
Sci-Hub is an evil website that pirated 85M+ research papers and made them freely available
And now they've added AI to their database to make Sci-Bot.
It answers your questions using latest, full-text articles.
But DO NOT use it. We should all try to make billion-dollar academic publishers richer.
I'm putting the link below so you know how to avoid it.
@VincentRK I think at this point you probably can't detect it. I would recommend asking for mass spectrometry proteomic validation of the antibody results. That is more difficult to fabricate at the moment.
Does identifying thousands of plasma proteins mean more biology? Not always. Over half of 210 published datasets contain contamination markers reported as disease biomarkers.
I’m looking for an automated way to read others’s scientific data without giving credit or acknowledgement, and also claim full credit for insights from it. And I want it to have a fitting name
OAI: say no more
https://t.co/3YC9Zxf7mL
Latest Bruker or Thermo #TeamMassSpec instrument for #proteomics? Please share your honest experience (anonymously if you want)! Sometimes it is more than just the specs (ie. price, robustness, maintenance, software)
#massspectrometry
Our latest paper with @atul_deshmukh1 is now out in @JPhysiol. We developed FibeRtypeR to infer muscle fiber type from transcriptome or proteome data. Would be great to receive feedback on it. @TeamDerave@ugent_fge
Paper: https://t.co/WDSU75oqD7
Tool: https://t.co/DmzukCywYw
Our latest #proteomics product to top off our Lys-C and Trypsin offerings.
A high quality optimal premix of Lys-C and Trypsin that enables reproducible proteomic analysis!
Make sure to follow @kpl_aps for more exciting news and updates! #TeamMassSpec
🚀 New #proteomics product: Trypsin/Lys-C Premium Mix
With the right formulation, you can add Lys-C and Trypsin together instead of sequentially.
🧪 One tube
⏱️One incubation
📉 Best-in-class missed cleavage rates
🌱 100% recombinant
https://t.co/XxqPY8CSuk
Our recombinant, animal free, sustainable, artisanal 😁 Trypsin is here! High quality and high performance, drop in replacement for any existing #proteomic workflows
Introducing Trypsin RMS - our third product is here.
Recombinant. Methylated. Sequencing-grade.
The robust Trypsin #proteomics labs need, without the batch variability of animal-derived alternatives.
Learn more: https://t.co/ZoanWdBGxi
#TeamMassSpec
It just seems implausible this is what we are made of, essentially, nanotechnology about a billion years beyond anything we can design or make ourselves.
Does enzyme quality matter in #proteomics?
The study demonstrate that LysC combined with Trypsin reduces missed cleavages, ie. from 30% → 15% . And the source, quality, sequence of LysC enzymes can vary!
For challenging samples, adding LysC can make a big difference!
New publication alert!📢
KPL collaborates w/ @JesperOlsenLab on the first systematic comparison of LysC enzymes for proteomics published in @JProteomeRes
A. lyticus LysC: >90% cleavage efficiency, >97% specificity
Read more: https://t.co/cTTYcHJ5Yn
#TeamMassSpec#proteomics