Tanveer Batth

Co-founder/CEO @KPL_ApS , Proteomics researcher in Copenhagen, Denmark @UCPH_health @NNFCPR . science, technology, culture, memes and vibes.

[Tanveer Batth @ucdmrt]

Our latest bioRxiv preprint characterizes our Arg-C Zero protease (available now at https://t.co/le1gAzp8bf 😏) What you may learn might surprise you!!1!! (ie. equivalent protein group ID's to Trypsin with half the peptide IDs)
#proteomics #teammasspsec

[KPL ApS @KPL_ApS]

🔬 New bioRxiv preprint: Arg-C Zero, recombinant arginyl endopeptidase with >99% cleavage efficiency, proline-adjacent cleavage, Histone and labile PTMs analysis like ADP-ribosylation and comparable proteome depth to Trypsin.

https://t.co/cpWFzASQLG
#Proteomics #TeamMassSpec

[Matthias Mann Lab @labs_mann]

1/5
Cell identity is written in the proteome, not in the DNA, and not always in the RNA. Out on bioRxiv today: The first cell type-resolved, MS-based proteomic atlas of the human body.
https://t.co/5RJ0nVoQ81

[Michael Steidel @michaelsteidel1]

#TeamMassSpec: any independent data on dual-column LC creating subtle batch effects in LFQ proteomics?

Thinking column A/B intensity shifts, missingness, RT drift, or condition-column confounding. Vendor claims are nice; real-world datasets would be nicer.

[Tanveer Batth @ucdmrt]

@michaelsteidel1 I think this place is pretty dead, I think you might get a more immediate response on Bluesky or https://t.co/vpW9oxqaia

[Tanveer Batth @ucdmrt]

🥴🥴🥴

[Sholto David @addictedtoigno1]

Surprised to discover that Thermo Fisher appears to show a fake western blot for the validation of one of their p53 antibodies. I've added a diagram to show the very similar bands. This does not appear to be one of the "published figures", but their own internal data.

[Mushtaq Bilal, PhD @MushtaqBilalPhD]

Sci-Hub is an evil website that pirated 85M+ research papers and made them freely available

And now they've added AI to their database to make Sci-Bot.

It answers your questions using latest, full-text articles.

But DO NOT use it. We should all try to make billion-dollar academic publishers richer.

I'm putting the link below so you know how to avoid it.

[Earth @earthcurated]

The Conehead Praying Mantis probably the craziest insect you have seen all day! | photo by Marta Albareda

[Tanveer Batth @ucdmrt]

@VincentRK I think at this point you probably can't detect it. I would recommend asking for mass spectrometry proteomic validation of the antibody results. That is more difficult to fabricate at the moment.

[Matthias Mann Lab @labs_mann]

Does identifying thousands of plasma proteins mean more biology? Not always. Over half of 210 published datasets contain contamination markers reported as disease biomarkers.

[Michael Baym @baym]

I’m looking for an automated way to read others’s scientific data without giving credit or acknowledgement, and also claim full credit for insights from it. And I want it to have a fitting name

OAI: say no more

[R/Proteomics @R_Proteomics]

https://t.co/3YC9Zxf7mL

Latest Bruker or Thermo #TeamMassSpec instrument for #proteomics? Please share your honest experience (anonymously if you want)! Sometimes it is more than just the specs (ie. price, robustness, maintenance, software)

#massspectrometry

[Tanveer Batth @ucdmrt]

@GooseData Looks like Trypsin .

[Thibaux Van der Stede @ThibauxV]

Our latest paper with @atul_deshmukh1 is now out in @JPhysiol. We developed FibeRtypeR to infer muscle fiber type from transcriptome or proteome data. Would be great to receive feedback on it. @TeamDerave @ugent_fge

Paper: https://t.co/WDSU75oqD7

Tool: https://t.co/DmzukCywYw

[Evgenii @joe_fenrir]

ThermoFisher in a nutshell

[Tanveer Batth @ucdmrt]

Our latest #proteomics product to top off our Lys-C and Trypsin offerings.
A high quality optimal premix of Lys-C and Trypsin that enables reproducible proteomic analysis!

Make sure to follow @kpl_aps for more exciting news and updates! #TeamMassSpec

[KPL ApS @KPL_ApS]

🚀 New #proteomics product: Trypsin/Lys-C Premium Mix

With the right formulation, you can add Lys-C and Trypsin together instead of sequentially.

🧪 One tube
⏱️One incubation
📉 Best-in-class missed cleavage rates
🌱 100% recombinant

https://t.co/XxqPY8CSuk

[Tanveer Batth @ucdmrt]

Our recombinant, animal free, sustainable, artisanal 😁 Trypsin is here! High quality and high performance, drop in replacement for any existing #proteomic workflows

[KPL ApS @KPL_ApS]

Introducing Trypsin RMS - our third product is here.
Recombinant. Methylated. Sequencing-grade.
The robust Trypsin #proteomics labs need, without the batch variability of animal-derived alternatives.
Learn more: https://t.co/ZoanWdBGxi
#TeamMassSpec

[Andrew Côté @Andercot]

It just seems implausible this is what we are made of, essentially, nanotechnology about a billion years beyond anything we can design or make ourselves.

[KPL ApS @KPL_ApS]

Trypsin is the workhorse of bottom-up proteomics. But when was it first discovered? 🤔🤔🤔

(Answer in the comments)

[Tanveer Batth @ucdmrt]

Does enzyme quality matter in #proteomics?

The study demonstrate that LysC combined with Trypsin reduces missed cleavages, ie. from 30% → 15% . And the source, quality, sequence of LysC enzymes can vary!

For challenging samples, adding LysC can make a big difference!

[KPL ApS @KPL_ApS]

New publication alert!📢
KPL collaborates w/ @JesperOlsenLab on the first systematic comparison of LysC enzymes for proteomics published in @JProteomeRes

A. lyticus LysC: >90% cleavage efficiency, >97% specificity

Read more: https://t.co/cTTYcHJ5Yn
#TeamMassSpec #proteomics

[눈 ︵ 눈 @apathei]

@MrEwanMorrison