Welker Lab

Official Twitter account of Ervin Welker's lab

[Welker Lab @welkerlab]

Proud to share that our latest work, „flexiAsCas12a and other Cas12a variants enhance the applicability of Cas12a nucleases in prime editing” is now available at Genome Biology.
https://t.co/jsAdoirInx
https://t.co/qpsWKde3qG
https://t.co/MnU96jvJAt

[Welker Lab @welkerlab]

We hope proPE contributes to the ever-improving prime editing toolkit, bringing prime editing closer to routine therapeutic use.

[Welker Lab @welkerlab]

Delighted to share our new method proPE (prime editing with prolonged editing window) is out in Nature Catalysis: https://t.co/JierTaoIvk
Kudos to first author Sarah Laura Krausz and all co-authors at HUN-REN TTK & HUN-REN SZBK! #CRISPR #primeediting

[Welker Lab @welkerlab]

proPE can greatly improve allele/gene specificity—even where classical PE is not effective.

[Peter Kulcsar @p_kulcsar]

In our recent work published in @NatureComms we demonstrated a strategy to efficiently edit practically any target without off-targets using SpCas9 variants. The ‘CRISPRecise set' of variants will be available as a kit from @Addgene. @welkerlab @BiochemBrc https://t.co/a7KZ7GXWxE

[Welker Lab @welkerlab]

@AltNHEJeh @biorxivpreprint Returning to this question, we checked and SuperFi-PE does not function with or without these mutations https://t.co/pK5liljSKW (Sup. Fig. 10). A possible explanation could be that the SuperFi H840A nickase (present in PE) shows very low activity (Fig. 4).

[Welker Lab @welkerlab]

In our recent @biorxivpreprint we report that SuperFi-Cas9 exhibits extremely high fidelity but reduced activity in mammalian cells https://t.co/8aUbuL8Reo

[Welker Lab @welkerlab]

In our new paper published in @NatureComms we characterize the on-target activity and off-target propensity of SuperFi-Cas9 in mammalian cells and also see strongly reduced activity but with high fidelity features. @BiochemBrc https://t.co/pK5liljSKW

[Peter Kulcsar @p_kulcsar]

In our recent @biorxivpreprint, using target-matched SpCas9 variants, we demonstrated efficient editing without off-targets on any SpCas9 target sequence, even on challenging targets that were attempted but failed in all previous studies. @BiochemBrc https://t.co/gGQ09UAGd7

[Welker Lab @welkerlab]

@davidwtaylorjr Thank you David! Congratulations on your work! Your excellent structures added a few pieces to the incomplete puzzle of the SpCas9 mechanism.

[Welker Lab @welkerlab]

@jeh_delivery @biorxivpreprint Thank you for your interest in our study! We are also puzzled by the lack of PE activity on targets where SuperFi nuclease is active and we can see your point, in fact we are already conducting this experiment alongside other approaches.

[Welker Lab @welkerlab]

@JohnDoench @Fatwa_Adikusuma @jpkbravo @JohnDoench We checked and SuperFi is compatible with an additional 5' G at the end of the spacer (Fig. 1g-h,https://t.co/yLGnPOmAjc. We assume that it tolerates a 5' G because of the Y1013D mutation (same aa. mutated as in Blackjack variants- Kulcsar et al., Nat. Commun, 2020)