Javier

ThinkDifferent!

[Javier @1avier]

@JudgeTheZipper Lo hiciste gracias a tu esfuerzo y a una educación pública y de calidad

[Javier @1avier]

@JudgeTheZipper Lo hiciste gracias a tu esfuerzo y a una educación pública de calidad.

[Javier @1avier]

@AgEInves @CienciaGob @CrueUniversidad @CSIC @SaludISCIII @FECYT_Ciencia @RaquelYotti @DomenecE Thank you @AgEInves for continuing to fund our research program

[Jakobi Lab 🇳🇱🇵🇹🇿🇦🇫🇷🇩🇪🇬🇷🇮🇳🇮🇹🇻🇳 @CryoTUD]

Do you like method development and want to work on a new generation of cryo-EM sample supports?

Join us for a 3-yr postdoctoral fellowship to find fun and supportive colleagues, free (and good) coffee, a great department and state-of-the-art facilities.

https://t.co/LkzY25BlFQ

[Mónica García @Monica_Garcia_G]

¿Ya sabéis con quién vais a ir? ☺️

[Javier @1avier]

@theNASciences @TexasScience WoW! Congrats @McLellan_Lab !!

[José Carrillo @pepcarrillo]

1) En pocos días la UCM nombrará a Isabel Díaz Ayuso “alumna ilustre”. He evitado hacer pública mi postura sobre dicho nombramiento con la esperanza de que no se llevaría a cabo. Hoy, pérdida toda expectativa, hago públicas las razones por las que discrepo de ese nombramiento.

[cryoEM Papers @cryoEM_Papers]

Cryo-EM map anisotropy can be attenuated by map post-processing and a new method for its estimation https://t.co/hV8cZKBGwV

[Javier @1avier]

@builab @dynein_awk @TheRealCSBlack @ahmad__khalifa Contrats Huy!

[Javier @1avier]

@delarosatrevin Enhorabuena Josemi! Todo lo mejor para tu nueva aventura americana ☺️

[Javier @1avier]

@IsraelF96135088 DeepEMHancer trains on a large set of different maps and LocScaled maps. I don't see why two specific DE and LS maps should be identical. Also, DeepEMhancer performs masking based on PDB maps. So DeepEMhancer = local scaling + tight masking

[Javier @1avier]

@CryoTUD @SjorsScheres @biochem_fan I would have to take a look to the code to refresh but I think the normalization is based on percentil values obtained from the maps ( (x-p5)/(p95-p5)) followed by a clipping process. This will compress the histogram

[Javier @1avier]

@SjorsScheres @biochem_fan @IUCrJ I have not read the paper in detail but I think authors claim to use the previous knowledge accumulated in deepEMhancer to improve the alignment and seems to work. I understand that the resolution is obtained from Relion maps

[Javier @1avier]

@CrollTristan Those low countour "features" (why not calling it noise?) are a consequence of chunking the map into cubes, and their intensities should be negligible compared to the rest of the volume. What is the contour level?

[Javier @1avier]

@CryoTUD @SjorsScheres @biochem_fan LocScale maps are normalized before training in DeepEMhancer. This is a standard approach in Deep Learning

[Javier @1avier]

@SjorsScheres @biochem_fan DeepEMhancer maps are not supposed to be used to evaluate resolution values. I think this is explicitly said in the DeepEMhancer paper. Agree that validation is key in cryoEM

[Javier @1avier]

@Yoshi__Ichikawa Contrats & good luck

[Javier @1avier]

@Elena_Salamanca Políticos tenemos muchos, Periodistas parece que no tantos. Usted debería ser (o al menos parecer) imparcial.