みたひろあ

みんな、それぞれ、歩いていく。

[みたひろあ @A_Kishu]

Friend or foe: The role of stress granule in neurodegenerative disease: Neuron https://t.co/MWSVzvOy1M

[岩波書店 @Iwanamishoten]

今日は詩人・評論家、吉本隆明の命日（2012年）。
その独創的な仕事の数々で現代思想界に多大な影響を及ぼした言わずと知れた戦後を代表する思想家ですが、詩と批評、文学と思想の統一を、詩の言葉で自立させた詩人でもありました。

蜂飼耳 編『吉本隆明詩集』☞ https://t.co/jGuR9um3Iy

[青木 航 / Wataru Aoki @mighty_tora]

クレイグ・ベンター研究所から、非生命から生命を合成する方法がプレプリントとして発表されました。人類がゼロから生命を創れるようなるための大きなマイルストーンです。
https://t.co/JpmPSVp1QV

[NEJM @NEJM]

𝐓𝐏𝟓𝟑 is the gene that encodes the tumor-suppressor protein p53. It is commonly mutated in human cancers, and as with other tumor-suppressor genes, loss of function in both alleles (i.e., two "hits") is required for tumorigenesis. Wild-type p53 protein is short-lived and expressed at low levels. Mutant p53 often has an extended life span and accumulates in cancer cells. The p53 protein is a transcription factor that controls the expression of hundreds of target genes by binding and activating specific target genes that, once transcribed and translated, suppress tumorigenesis. Mutant p53 has limited or no tumor-suppressive function because it cannot bind and activate target genes.

To learn more about this NEJM Illustrated Glossary term, read the editorial “Restoring Function to a Variant of p53 in Solid Tumors” by Xin Lu, PhD, from @Ludwig_Cancer: https://t.co/5Ox6Kuax6Q

Explore more terms: https://t.co/kobNxmaQrY

[nature @Nature]

Nature research paper: OR7A10 GPCR engineering boosts CAR-NK therapy against solid tumours

https://t.co/Ei3AdAGOpA

[Ｊｕｎ Ｙａｓｕｄａ @jyasuda1]

これまでもタンパク質の構造から分子間相互作用を推定するシステムはあったが、二桁くらい性能を上げたFoldMasonを開発したとする論文。Science誌。

https://t.co/C89e3jEu0M

[Niko McCarty. @NikoMcCarty]

The model of gene expression taught in school is highly misleading!

Transcription factors are proteins that bind to DNA and then help repress, or activate, the expression of genes. Cells have hundreds of different types of transcription factors, each tuned to regulate different genes based on short snippets of DNA located near those genes.

The basic model, taught in school, says that these transcription factor proteins float around the cell and, when they bump into a DNA sequence, either latch onto it strongly (CORRECT SITE!) or fall off quickly (WRONG SITE) and keep searching. All the other DNA in a cell is basically abstracted away as unimportant or irrelevant; mere background noise.

But again, this model is naive! And a new paper, published in Cell, beautifully shows how the sequences SURROUNDING a transcription factor's binding site also matter a great deal.

This won't be surprising to many biologists, as "cracks" in the standard two-state model began emerging decades(?) ago. Biologists have tagged transcription factors with fluorescent tags and then watched them move around living cells. And they have noticed that when transcription factors land in a "wrong" location in the genome, they skip or hop to a nearby location and repeat this until finally connecting with the "correct" sequence. So in other words, there are actually three states that a transcription factor can exist in: free-floating, "searching", or "bound."

(More technically, transcription factors first do a 3D search, then latch onto DNA and do a 1D search to find the correct location.)

For this new paper, though, scientists exhaustively quantified *how* the sequences flanking a transcription factor binding site influence the search of the protein.

They did a huge in vitro experiment, wherein they placed a specific transcription factor with a known binding site, called KLF1, in a huge library of 11,812 different DNA sequences. These sequences had mutated "core" binding sites and variations in the flanking sequences. They also prepared negative controls. Then, these researchers measured the binding kinetics of KLF1 with each sequence to understand which bases in the flanking sites impact the 1D search.

What they found is that KLF1 has a basically flat disocciation rate from its core sequence, but that the PROBABILITY that it finds this sequence depends a lot on the surrounding context. Even mutations located dozens of bases away from the core site matter a lot, either pushing KLF1 to "hop" faster to find the site, or "trapping" KLF1 and slowing down its search. These flanking sequences can cause up to a 40-fold variation in the affinity of a transcription factor for its target site!

This is just one small part of the paper, though, so I encourage anyone interested to read the whole thing. It is challenging throughout.

[eLife - the journal @eLife]

Toxoplasma gondii carefully balances calcium between its organelles. This study shows the ER, via the SERCA pump, is central to controlling Ca²⁺ in the cytosol and mitochondria.

🔗 https://t.co/g4Alj28Wtj

[羊土社 教科書編集部 @Yodosha_text]

mRNAとタンパク質どちらが大きい？
転写と翻訳、どちらの方が速い？
生物にはどのくらいの細胞が存在する？

そんな疑問にお答えする、"数でとらえる"細胞生物学の書籍です
https://t.co/YdnOUodsiP

[MLB Japan @MLBJapan]

【 #字幕付き動画 】試合中、監督に吠えるマックス・シャーザーが今ポストシーズンでは話題になりましたが、今回シャーザー登板日の暗黙のルールをチームメイトが語る貴重な映像が到着！
登板日は「絶対話しかけるな」というルールに例外は？チームメイトたちが失敗したことは？マッドマックスの異名で知られるシャーザーの��側を聞いてみましょう！🤫

[Cell @CellCellPress]

In the latest issue! Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains https://t.co/j4XBmyav91

[Science Magazine @ScienceMagazine]

From carbon dioxide to starch—no plants required.

In Science, researchers developed a cell-free method of synthesizing starch from CO2 and hydrogen using a combination of chemical catalysts and a carefully selected set of enzymes.

Learn more: https://t.co/jZBkJCwqYJ #ScienceMagArchives

[MLB Japan @MLBJapan]

【 #ブルージェイズ 】これぞ"マッド・マックス"と呼ばれる所以😳
マックス・シャーザーが5回途中に交代を告げられそうになるも、マウンドに向かうシュナイダー監督を怒鳴って追い返し、交代拒否の続投。そしてアロザレーナを三振に斬り、雄叫びを上げました😤
#ポストシーズン2025

[eLife - the journal @eLife]

Exercise can reduce pain, but you don’t have to work out harder to feel the benefit.

New research suggests that fitness level and sex may play a bigger role than exercise intensity in how we experience exercise-induced pain relief.
https://t.co/YM9kieMcN2

[FOX Sports: MLB @MLBONFOX]

Half of the playoff field has been clinched! 🍾

RT if your team will be playing Postseason baseball! 🍂

[東畑 開人 @ktowhata]

勇気を意味する”courage”の語源が、ラテン語で心や心臓を意味するcorであるのは知っていたが、さらには「心にある思いをすべて口にして伝えること」というのが元の意味だったらしい。言葉にすることが勇気の根幹にあったというのは面白いな。

[吉高由里子 @ystk_yrk]

見るのと
出るのとでは
全然違う！！！😂😂😂

お邪魔しました💦

今夜です☺️🙏🏼

[みたひろあ @A_Kishu]

ロッキング・オン・グループ��表・渋谷陽一さん死去　74歳　2023年に脳出血発症　リハビリ続けるも「74年の生涯を終えました」【報告全文】 | ORICON NEWS https://t.co/qrM40rkET3

[MLB Japan @MLBJapan]

【 #ブレーブス 】どういうこと！？
ロナルド・アクーニャJrが軽く返球した球が��の投げ方と全く合ってない伸び方を見せ、それに惑わされたであろうランナーを3塁で刺しました💥

[みたひろあ @A_Kishu]

心から尊敬し、応援しています！

[池江 璃花子 @rikakoikee]

コツコツやってきた足の強化。
陸では強くなってるけど、スタートとドルフィンキックになるとやっぱり力の伝達がうまく行かない。
悔しいけど、今年は泳ぎで挽回します。